A5071752676- Polyomavirus and related diseases
- Cervical Cancer and HPV Research
- Full-Duplex Wireless Communications
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Head and Neck Cancer Studies
- Multiple Sclerosis Research Studies
- Oral Health Pathology and Treatment
- Plant Virus Research Studies
- Energy Harvesting in Wireless Networks
- Viral gastroenteritis research and epidemiology
- Cytomegalovirus and herpesvirus research
- Hepatitis C virus research
- HIV/AIDS drug development and treatment
- Viral Infections and Immunology Research
- MicroRNA in disease regulation
- Biochemical and Molecular Research
Helsinki University Hospital
2016-2018
University of Helsinki
2016-2018
Using small RNA sequencing of libraries established from cervical samples and cancer cell lines, we have previously reported identification nine validation five putative microRNA species encoded by human papillomaviruses (HPV) including microRNAs HPV 16. Here studied the expression 16 in containing lines. Different sample matrices were collected for study: 20 paraffin embedded tissue samples, liquid cytology women attending colposcopy due to abnormalities, as well four Total was extracted,...
BK polyomavirus (BKPyV) infection is a common asymptomatic viral in the general population. Severe complications are seen immunocompromised individuals, such as polyomavirus-associated nephropathy (PyVAN) renal transplant recipients. Information on BKPyV microRNA expressions scarce, although polyomavirus-encoded microRNAs have been shown to control replication and assist immune evasion. Whereas pathogenic role of rearrangements JC has well established, little known about PyVAN. To assess...
Progressive multifocal leukoencephalopathy (PML) is a fatal disease caused by reactivation of JC polyomavirus (JCPyV) in immunosuppressed individuals and lytic infection neurotropic JCPyV glial cells. The exact content mutations within individual strains has not been studied to our knowledge.We exploited the capacity single-molecule real-time sequencing technology determine sequence complete genomes single reads. method was used precisely characterize 3 patients with PML without bias...
The aim was to assess the performance of two commercial assays for detection high-risk human papillomavirus (hrHPV): Aptima HPV Assay (Hologic, Inc., Marlborough, MA, USA) which detects mRNA 14 different hrHPV types, and Hybrid Capture 2 DNA test (HC2; Qiagen, Gaithersburg, MD, USA), 13 types. Test compared in settings a standard colposcopy clinic, among regular patient flow.Two separate cervical cell samples HC2 testing were collected from women referred or follow-up visit. Altogether, 481...
This study evaluates the suitability of Xpert HPV (Cepheid, Sunnyvale, CA , USA ) test for cervical and tonsillar formalin‐fixed paraffin‐embedded ( FFPE tissue samples as compared to tests currently used in diagnostics. Cervical biopsies liquid cytology LC were collected from 48 women attending colposcopy. Biopsies processed histology tested hr using . Hybrid Capture 2 HC 2; Qiagen, Hilden, Germany) tests. Also 29 archived carcinoma Xpert, results with immunohistochemical p16 INK 4a (p16)...
Graphical abstractAbstractHepatitis C virus (HCV) is a globally significant blood-borne agent causing liver diseases, and it has infected over 170 million people worldwide. HCV diverse group of RNA viruses currently divided into genotypes 1–7 as well subtypes. infection can be treated with antiviral drugs, but the genotype to determined for optimal selection treatment strategy. The aim this study was set up sequencing-based genotyping method suitable workflow diagnostic laboratory....