A5032553934- Cervical Cancer and HPV Research
- Polyomavirus and related diseases
- Full-Duplex Wireless Communications
- Molecular Biology Techniques and Applications
- Energy Harvesting in Wireless Networks
- Cancer-related molecular mechanisms research
- Genital Health and Disease
- Virus-based gene therapy research
- MicroRNA in disease regulation
- T-cell and Retrovirus Studies
- Head and Neck Cancer Studies
- Plant Virus Research Studies
- NF-κB Signaling Pathways
- Reproductive tract infections research
- Animal Virus Infections Studies
- Hepatitis B Virus Studies
- Immune Response and Inflammation
- Antenna Design and Analysis
- Bacteriophages and microbial interactions
- Parvovirus B19 Infection Studies
- Neurofibromatosis and Schwannoma Cases
- Oral Health Pathology and Treatment
- Poxvirus research and outbreaks
- Cell Adhesion Molecules Research
- Urinary and Genital Oncology Studies
University of Helsinki
2015-2024
Helsinki University Hospital
2015-2024
Karolinska Institutet
2001
Finnish Red Cross
1999
DKFZ-ZMBH Alliance
1997-1998
German Cancer Research Center
1996-1998
Heidelberg University
1998
University of Turku
1989-1994
Fresh-frozen biopsies were obtained from 61 patients at diagnosis of squamous cell carcinoma the head and neck (HNSCC) for study prevalence physical status human papillomavirus (HPV) DNA. The frequency HPV DNA genotypes determined by SPF10 PCR screening with a general probe hybridization INNO-LiPA genotyping assay. In addition, single-phase primers FAP 59/64 nested CP 65/70 66/69 served to detect particularly cutaneous types. By sensitive assay, 37 (61%) samples positive HPV. HPV-16 was most...
Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in variety sample types and infectious syndromes. mHTS still its early stages translating into clinical application. To support development, implementation standardization procedures virus diagnostics, European Society Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim ENNGS to bring together...
Human papillomavirus (HPV) infection is a prerequisite of developing cervical cancer, approximately half which are associated with HPV type 16. 16 encodes three oncogenes, E5, E6, and E7, E5 the least studied so far. Its roles in regulating replication pathogenesis not fully understood. Here we utilize high-throughput screening to coordinately investigate effect on expression host protein-coding microRNA genes. MicroRNAs form class 22nt long noncoding RNAs regulatory activity. Among altered...
We report here identification and validation of the first papillomavirus encoded microRNAs expressed in human cervical lesions cell lines. established small RNA libraries from ten associated including cancer two harboring These were sequenced using SOLiD 4 technology. used sequencing data to predict putative viral discovered nine microRNAs. Validation was performed for five candidates, four which successfully validated by qPCR tissue samples lines: HPV 16, one 38 68. The expression 16...
JC polyomavirus (JCPyV) persists asymptomatic in more than half of the human population. Immunocompromising conditions may cause reactivation and acquisition neurotropic rearrangements viral genome, especially non-coding control region (NCCR). Such rearranged JCPyV strains are strongly associated with development progressive multifocal leukoencephalopathy (PML). Using next-generation sequencing (NGS) bioinformatics tools, NCCR was characterized cerebrospinal fluid (CSF; N=21) brain tissue...
Abstract Progressive multifocal leukoencephalopathy (PML) is a rare neurological condition associated with reactivation of dormant JC polyomavirus (JCPyV). In this study, we characterized gene expression and JCPyV rearrangements in PML brain tissue. Infection white matter astrocytes oligodendrocytes as well occasional cortex neurons was shown. harbored exclusively rearranged variants. Viral transcripts covered the whole genome on both strands. Strong differential human genes...
The incidence of oropharyngeal squamous cell carcinoma (OPSCC) has increased over the past decades in many western countries. This trend is mainly attributed to human papillomavirus (HPV). Cancer-related actions immunological defense systems are being intensively researched. Human toll-like receptors (TLRs) a family pattern recognition that participate against pathogens, but their also linked cancer. expression TLRs cervical epithelium alters both during clearance HPV infection and...
Using small RNA sequencing of libraries established from cervical samples and cancer cell lines, we have previously reported identification nine validation five putative microRNA species encoded by human papillomaviruses (HPV) including microRNAs HPV 16. Here studied the expression 16 in containing lines. Different sample matrices were collected for study: 20 paraffin embedded tissue samples, liquid cytology women attending colposcopy due to abnormalities, as well four Total was extracted,...
We studied the prevalence of HPV DNA among university students within Helsinki metropolitan area in Finland. First-y attending a general health examination as well visiting practitioner for contraception were enrolled. Vaginal self-samples or cervicovaginal swabs collected from total 1469 students. was determined using liquid hybridization test. Of all 33.0% positive DNA. samples 84.3% high-risk HPV. The study demonstrated strikingly high young women. Self-collected proved to be acceptable...
High-grade cervical squamous intraepithelial lesions (CIN) as well cell carcinoma and adenocarcinoma of the cervix are associated with persistent high-risk human papillomavirus (HPV) infection.A number cellular events play a role in HPV pathogenesis development lesions, including alterations adhesion motility.The crucial plasma membrane -cytoskeleton linker protein ezrin Ezrin-Radixin-Moesin (ERM) family is involved regulation morphology, invasion.Based on our previous work ERM proteins we...
BK polyomavirus (BKPyV) infection is a common asymptomatic viral in the general population. Severe complications are seen immunocompromised individuals, such as polyomavirus-associated nephropathy (PyVAN) renal transplant recipients. Information on BKPyV microRNA expressions scarce, although polyomavirus-encoded microRNAs have been shown to control replication and assist immune evasion. Whereas pathogenic role of rearrangements JC has well established, little known about PyVAN. To assess...
Sensitive and specific detection of human papillomaviruses (HPV) in cervical samples is a useful tool for the early diagnosis epithelial neoplasia anogenital lesions. Recent studies support feasibility HPV DNA testing instead cytology (Pap smear) as primary test population screening cancer. This likely to be an option near future many countries, it would increase efficiency abnormalities. We present here microarray typing 15 most important high-risk types two low risk types. The method based...
Objective The malignant transformation rate of recurrent respiratory papillomatosis (RRP), a disease caused by human papillomavirus (HPV), has varied significantly. Cells the immune system express toll‐like receptors (TLRs) that recognize particles from viruses and bacteria; TLRs are also present on tumor cells, down‐regulation been shown during progression HPV‐associated neoplasia. aim this study was to determine laryngeal papillomas (LPs) analyze potential TLR2, TLR4, TLR9 immunoexpression...