A5067785013- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Infant Nutrition and Health
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Clostridium difficile and Clostridium perfringens research
- Digestive system and related health
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Hepatitis Viruses Studies and Epidemiology
- Respiratory viral infections research
- Gut microbiota and health
- Escherichia coli research studies
- Energy Harvesting in Wireless Networks
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Advanced Thermodynamic Systems and Engines
- Congenital Anomalies and Fetal Surgery
- Monoclonal and Polyclonal Antibodies Research
- Microbial Metabolites in Food Biotechnology
- Viral Infectious Diseases and Gene Expression in Insects
- Pediatric Hepatobiliary Diseases and Treatments
Universitat de València
2016-2025
INCLIVA Health Research Institute
2016-2025
Hospital Clínico Universitario de Valencia
2003-2024
Centro de Investigacion Principe Felipe
2007-2020
Indiana Athletic Trainers Association
2012-2015
Instituto de Agroquímica y Tecnología de Alimentos
2010-2015
Consejo Superior de Investigaciones Científicas
2010-2013
University of Turku
2013
Instituto Venezolano de Investigaciones Científicas
2006-2010
Linköping University
2006-2009
The gut microbiota has emerged as a key factor in the pathogenesis of intestinal viruses, including enteroviruses, noroviruses and rotaviruses (RVs), where stimulatory inhibitory effects on infectivity have been reported. With aim determining whether members interact with RVs during infection, combination anti-RV antibody labeling, fluorescence-activated cell sorting 16S rRNA amplicon sequencing was used to characterize interaction between specific bacteria RV stool samples children...
ABSTRACT The molecular epidemiology of human caliciviruses (HuCVs) causing sporadic cases and outbreaks acute gastroenteritis around eastern Spain (Catalonia the Valencian Community) was studied by reverse transcription-PCR (RT-PCR) sequencing part RNA polymerase gene in open reading frame 1. HuCVs were detected 44 310 stool specimens (14.19%) negative for other enteric pathogens obtained from children with gastroenteritis. Norwalk-like viruses (NLVs) most common cause investigated here....
Rotavirus (RV) is the major cause of severe gastroenteritis in young children. A virus-encoded enterotoxin, NSP4 proposed to play a role causing RV diarrhoea but how can induce emesis, hallmark illness, remains unresolved. In this study we have addressed hypothesis that RV-induced secretion serotonin (5-hydroxytryptamine, 5-HT) by enterochromaffin (EC) cells plays key emetic reflex during infection resulting activation vagal afferent nerves connected nucleus solitary tract (NTS) and area...
In November 2004, 116 individuals in an elderly nursing home El Grao de Castellón, Spain were symptomatically infected with genogroup II.4 (GII.4) norovirus. The global attack rate was 54.2%. Genotyping of 34 symptomatic regarding the FUT2 gene revealed that one patient was, surprisingly, a non-secretor, hence indicating secretor-independent infection. Lewis genotyping Lewis-positive and negative susceptible to norovirus infection status did not predict susceptibility. Saliva based ELISA...
Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in variety sample types and infectious syndromes. mHTS still its early stages translating into clinical application. To support development, implementation standardization procedures virus diagnostics, European Society Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim ENNGS to bring together...
Fecal-orally transmitted gastroenteritis viruses, particularly human noroviruses (HuNoVs), are a public health concern. Viral transmission risk through contaminated water results underexplored as they have remained largely unculturable until recently and the robust measuring of viruses infectivity in single cell line is challenging. This study primarily aimed to test feasibility intestinal enteroids (HIE) model demonstrate multiple wastewater. Initially, key factors affecting viral...
Histo-blood group antigens and secretor status have been associated with susceptibility to Norovirus infections, which suggests that antibody prevalence titer might correlate these phenotypes.Plasma samples (n = 105) from Swedish blood donors had genotyped for (FUT2) Lewis (Le; FUT3) genotypes phenotyped ABO Le groups were analyzed immunoglobulin G titers norovirus genogroup (GG) II.4.The results showed nonsecretors (se4128se428) Lea+b- individuals not only significantly lower than did...
The detection and molecular characterization of pathogenic human viruses in urban sewage have been used extensively to derive information on circulating given populations throughout the world. In this study, a similar approach was applied provide an overview epidemiology waterborne gastroenteritis areas Caracas, capital city Venezuela South America. Dry season sampling conducted sewers major river severely polluted with discharges. Nested PCR for adenoviruses (HAds), while reverse...
The carbohydrate binding characteristics of a norovirus GII.3 (Chron1) and GII.4 (Dijon) strain were investigated using virus-like particles (VLPs) saliva samples from 81 individuals genotyped for FUT2 (secretor) FUT3 (Lewis) phenotyped ABO Lewis blood groups. two VLPs showed typical secretor-gene-dependent bound significantly stronger to A, B, AB than O (P < 0.0001 P 0.001) but did not bind any secretor-negative individuals. larger interindividual variation B A2 secretors 0.01). When...
Three putative α-L-fucosidases encoded in the Lactobacillus casei BL23 genome were cloned and purified. The proteins displayed different abilities to hydrolyze natural fucosyloligosaccharides like 2'-fucosyllactose, H antigen disaccharide, type II trisaccharide, 3'-, 4'-, 6'-fucosyl-GlcNAc. This indicated a possible role utilization of oligosaccharides present human milk intestinal mucosa.
Host genetic factors, such as histo-blood group antigens (HBGAs), are associated with susceptibility to norovirus (NoV) and rotavirus (RV) infections. Recent advances point the gut microbiome a key player necessary for viral pathogen cause infection. In vitro NoV attachment host cells resulting infections have been linked interactions certain bacterial types in microbiota. We investigated relationship between genotype, microbiota, Saliva fecal samples from 35 adult volunteers were analysed...
Summary The probiotic L actobacillus casei catabolizes galacto‐ N ‐biose ( GNB ) and lacto‐ LNB by using a transport system metabolic routes different from those of B ifidobacterium . contains gene cluster, gnbREFGBCDA , involved in the metabolism also ‐acetylgalactosamine. Inactivation gnbC EIIC or ptsI (Enzyme I phosphoenolpyruvate : sugar phosphotransferase PTS prevented growth on three carbohydrates, indicating that they are transported phosphorylated same Gnb Enzyme activities analysis...
The aim of the present study was to perform molecular epidemiology rotaviruses and noroviruses detected in sewage samples from a large wastewater facility city Valencia, Spain. A total 46 were collected over one-year period (September 2016 September 2017). Norovirus rotavirus quantified by RT-qPCR, genotyped semi-nested RT-PCR further characterized sequencing phylogenetic analyses. Noroviruses widely distributed (69.6% for norovirus GI, 76.0% GII, 71.7% rotaviruses) viral loads varied 4.33...
Whether antibody levels measured by commercially available enzyme or chemiluminescent immunoassays targeting the SARS-CoV-2 spike (S) protein can act as a proxy for serum neutralizing activity remains to be established many of these assays. We evaluated degree correlation between antibodies (NtAb) binding and SARS-CoV-2-S-IgG four commercial in sera drawn from hospitalized COVID-19 patients. Ninety 51 patients were tested pseudotyped virus neutralization assay, LIAISON S1/S2 IgG, Euroimmun...
To improve the efficacy of intercalating dyes to distinguishing between infectious and inactivated hepatitis A virus (HAV) in food.
Rotavirus is the leading agent causing acute gastroenteritis in young children, with P[8] genotype accounting for more than 80% of infections humans. The molecular bases binding VP8* domain from VP4 spike protein to its cellular receptor, secretory H type-1 antigen (Fuc-α1,2-Gal-β1,3-GlcNAc; H1), and precursor lacto-N-biose (Gal-β1,3-GlcNAc; LNB) have been determined. resolution crystal structures complex H1 LNB site-directed mutagenesis experiments revealed that both glycans bind through a...
Group A rotaviruses are a major cause of acute gastroenteritis in children. The diversity and unequal geographical prevalence rotavirus genotypes have been linked to histo-blood group antigens (HBGAs) different human populations. In order evaluate the role HBGAs infections our population, secretor status (FUT2+), ABO blood group, Lewis were determined children attended for Valencia, Spain. During three consecutive years (2013-2015), stool saliva samples collected from 133 with infection....
Viral infections, particularly human norovirus (NoV), are a leading cause of diarrheal diseases globally. To better understand NoV susceptibility, it is crucial to investigate both host glycobiology and the influence microbiota. Histo-blood group antigens (HBGA) displayed on surfaces cells act as receptors, while certain bacteria express HBGA-like substances, facilitating virus-bacteria interactions. identify bacterial species interacting with during infection, stool samples from children...