A5078994269- Probiotics and Fermented Foods
- Infant Nutrition and Health
- Digestive system and related health
- Microbial Metabolites in Food Biotechnology
- Viral gastroenteritis research and epidemiology
- Gut microbiota and health
- Bacterial Genetics and Biotechnology
- Clostridium difficile and Clostridium perfringens research
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Microbial Metabolic Engineering and Bioproduction
- Amino Acid Enzymes and Metabolism
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Cancer-related gene regulation
- Escherichia coli research studies
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Phytase and its Applications
- Pediatric Hepatobiliary Diseases and Treatments
- RNA and protein synthesis mechanisms
- Diet, Metabolism, and Disease
- Protein Hydrolysis and Bioactive Peptides
- Epigenetics and DNA Methylation
- Microbial Natural Products and Biosynthesis
Instituto de Agroquímica y Tecnología de Alimentos
2016-2025
Consejo Superior de Investigaciones Científicas
2004-2021
Indiana Athletic Trainers Association
2010-2014
Wayne State University
1993-1997
Universidad de Oviedo
1988-1995
The intestinal microbiota are pivotal in determining the developmental, metabolic and immunological status of mammalian host. However, tract may also accommodate pathogenic organisms, including helminth parasites which highly prevalent most tropical countries. Both microbes helminths must evade or manipulate host immune system to reside environment, yet whether they influence each other’s persistence remains unknown. We now show that abundance Lactobacillus bacteria correlates positively...
The gut microbiota has emerged as a key factor in the pathogenesis of intestinal viruses, including enteroviruses, noroviruses and rotaviruses (RVs), where stimulatory inhibitory effects on infectivity have been reported. With aim determining whether members interact with RVs during infection, combination anti-RV antibody labeling, fluorescence-activated cell sorting 16S rRNA amplicon sequencing was used to characterize interaction between specific bacteria RV stool samples children...
The entire genome of Lactobacillus casei BL23, a strain with probiotic properties, has been sequenced. genomes BL23 and the industrially used Shirota YIT 9029 (Yakult) seem to be very similar.
Three putative α-L-fucosidases encoded in the Lactobacillus casei BL23 genome were cloned and purified. The proteins displayed different abilities to hydrolyze natural fucosyloligosaccharides like 2'-fucosyllactose, H antigen disaccharide, type II trisaccharide, 3'-, 4'-, 6'-fucosyl-GlcNAc. This indicated a possible role utilization of oligosaccharides present human milk intestinal mucosa.
Summary The probiotic L actobacillus casei catabolizes galacto‐ N ‐biose ( GNB ) and lacto‐ LNB by using a transport system metabolic routes different from those of B ifidobacterium . contains gene cluster, gnbREFGBCDA , involved in the metabolism also ‐acetylgalactosamine. Inactivation gnbC EIIC or ptsI (Enzyme I phosphoenolpyruvate : sugar phosphotransferase PTS prevented growth on three carbohydrates, indicating that they are transported phosphorylated same Gnb Enzyme activities analysis...
L-Fucose is a sugar present in human secretions as part of milk oligosaccharides, mucins, and other glycoconjugates the intestinal epithelium. The genome probiotic Lactobacillus rhamnosus GG (LGG) carries gene cluster encoding putative L-fucose permease (fucP), catabolic pathway (fucI, fucK, fucU, fucA), transcriptional regulator (fucR). metabolism LGG results 1,2-propanediol production, their fucI fucP mutants displayed severe mild growth defect on L-fucose, respectively. Transcriptional...
Rotavirus is the leading agent causing acute gastroenteritis in young children, with P[8] genotype accounting for more than 80% of infections humans. The molecular bases binding VP8* domain from VP4 spike protein to its cellular receptor, secretory H type-1 antigen (Fuc-α1,2-Gal-β1,3-GlcNAc; H1), and precursor lacto-N-biose (Gal-β1,3-GlcNAc; LNB) have been determined. resolution crystal structures complex H1 LNB site-directed mutagenesis experiments revealed that both glycans bind through a...
Sites of cytosine methylation are known to be hot spots for C.G T.A mutations in a number systems, including human cells. Traditionally, spontaneous hydrolytic deamination 5-methylcytosine thymine has been invoked as the cause this phenomenon. We show here that bacterial methyltransferase can convert DNA and reaction creates mutational spot at site methylation. The is fairly insensitive methyl donor reaction, S-adenosylmethionine. In many cancers, most frequent class C T changes within CG...
Genome analysis of Lactobacillus casei BL23 revealed that, compared to L. ATCC 334, it carries a 12.8-kb DNA insertion containing genes involved in the catabolism cyclic polyol myo-inositol (MI). Indeed, 334 does not ferment MI, whereas strain is able utilize this carbon source. The inserted consists an iolR gene encoding DeoR family transcriptional repressor and divergently transcribed iolTABCDG1G2EJK operon, complete MI catabolic pathway, which iolK probably codes for malonate semialdehyde...
ABSTRACT We have previously characterized from Lactobacillus casei BL23 three α- l -fucosidases, AlfA, AlfB, and AlfC, which hydrolyze in vitro natural fucosyl-oligosaccharides. In this work, we shown that L. is able to grow the presence of fucosyl-α-1,3- N -acetylglucosamine (Fuc-α-1,3-GlcNAc) as a carbon source. Interestingly, excretes -fucose moiety during growth on Fuc-α-1,3-GlcNAc, indicating only being metabolized. Analysis genomic sequence shows downstream alfB , encodes -fucosidase...
ABSTRACT AlfB and AlfC α-l-fucosidases from Lactobacillus casei were used in transglycosylation reactions, they showed high efficiency synthesizing fucosyldisaccharides. activities exclusively produced fucosyl-α-1,3- N -acetylglucosamine fucosyl-α-1,6- -acetylglucosamine, respectively. The reaction kinetics that can convert 23% p -nitrophenyl-α- l -fucopyranoside into at up to 56% -acetylglucosamine.
Noroviruses (NoVs) are the main etiologic agents of acute epidemic gastroenteritis and probiotic bacteria have been reported to exert a positive effect on viral diarrhea. The protruding (P) domain from NoVs VP1 capsid protein has ability assemble into so-called P-particles, which retain binding host receptors. We purified P-domains genotypes GI.1 GII.4 as 6X(His)-tagged proteins determined that, similar native domains, they were structured P-particles that functional in recognition specific...
The gastrointestinal microbiota members produce α-l-fucosidases that play key roles in mucosal, human milk, and dietary oligosaccharide assimilation. Here, 36 open reading frames (ORFs) coding for putative belonging to glycosyl hydrolase family 29 (GH29) were identified through metagenome analysis of breast-fed infant fecal microbiome. Twenty-two those ORFs showed a complete sequence with deduced amino acid sequences displaying the highest degree identity from Bacteroides thetaiotaomicron,...
Viral infections, particularly human norovirus (NoV), are a leading cause of diarrheal diseases globally. To better understand NoV susceptibility, it is crucial to investigate both host glycobiology and the influence microbiota. Histo-blood group antigens (HBGA) displayed on surfaces cells act as receptors, while certain bacteria express HBGA-like substances, facilitating virus-bacteria interactions. identify bacterial species interacting with during infection, stool samples from children...
The AlfB α-L-fucosidase from Lacticaseibacillus paracasei exhibits high specificity on fucosyl-α1,3-N-acetylglucosamine, achieving yields of 30 % in transfucosylation reactions for its synthesis. By random mutagenesis we selected variants with enhanced activity. Expression a collection alfB mutants E. coli resulted the isolation eighteen clones reduced activity p-nitrophenyl-α-L-fucopyranoside. carried diverse amino substitutions, leading to modifications their enzymatic parameters. In some...
Two novel phytases have been characterized from Bifidobacterium pseudocatenulatum and longum subsp. infantis. The enzymes belong to a new subclass within the histidine acid phytases, are highly specific for hydrolysis of phytate, render myo-inositol triphosphate as final product. They represent first this group probiotic microorganisms, opening possibilities their use in processing high-phytate-content foods.
ABSTRACT Human milk oligosaccharides (HMOs) are considered to play a key role in establishing and maintaining the infant gut microbiota. Lacto- N -triose forms part of both type 1 2 HMOs also glycan moieties glycoproteins. Upstream previously characterized gene cluster involved lacto- -biose galacto- metabolism from Lactobacillus casei BL23, there two genes, bnaG manA , encoding β- -acetylglucosaminidase precursor mannose-6-phosphate isomerase, respectively. In this work, we show that L. is...
The phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) represents hitherto the only example of group translocation transport systems. PTS transporters are exclusively found in bacteria and can be grouped on basis sequence structure into six classes. We have analyzed evolution mannose-class transporters. These a limited distribution among being mostly harbored by species associated to animals. results obtained indicate that these genes undergone complex evolutionary history,...