A5018450355- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Salmonella and Campylobacter epidemiology
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Amino Acid Enzymes and Metabolism
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Probiotics and Fermented Foods
- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Escherichia coli research studies
- Bacterial Infections and Vaccines
- Galectins and Cancer Biology
- Metabolism and Genetic Disorders
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Otolaryngology and Infectious Diseases
- SARS-CoV-2 and COVID-19 Research
- Bacterial biofilms and quorum sensing
- Veterinary medicine and infectious diseases
- Vibrio bacteria research studies
- Protease and Inhibitor Mechanisms
- Pneumocystis jirovecii pneumonia detection and treatment
- Infant Nutrition and Health
ETH Zurich
2019
University of Fribourg
2018
Inserm
2018
Microbiologie de l’alimentation au service de la santé
2013-2016
AgroParisTech
2013-2016
Université Paris-Saclay
2016
University of Basel
2012-2014
Université de Strasbourg
2014
Centre National de la Recherche Scientifique
2003-2013
Institut National de la Recherche Agronomique du Niger
2010
Pathogen access to host nutrients in infected tissues is fundamental for pathogen growth and virulence, disease progression, infection control. However, our understanding of this crucial process still rather limited because experimental conceptual challenges. Here, we used proteomics, microbial genetics, competitive infections, computational approaches obtain a comprehensive overview Salmonella nutrition mouse typhoid fever model. The data revealed that accessed an unexpectedly diverse set...
The entire genome of Lactobacillus casei BL23, a strain with probiotic properties, has been sequenced. genomes BL23 and the industrially used Shirota YIT 9029 (Yakult) seem to be very similar.
Invasive Salmonella infection is an important health problem that worsening because of rising antimicrobial resistance and changing serovar spectrum. Novel vaccines with broad coverage are needed, but suitable protective antigens remain largely unknown. Here, we tested 37 broadly conserved in a mouse typhoid fever model, identified antigen candidates conferred partial protection against lethal disease. Antigen properties such as high vivo abundance or immunodominance convalescent individuals...
In silico analysis of the Bifidobacterium breve UCC2003 genome allowed identification four genetic loci, each which specifies a putative enzyme II (EII) protein phosphoenolpyruvate:sugar phosphotransferase system. The EII encoded by fruA, clear homologue unique EIIBCA longum NCC2705 genome, was studied in more detail. fruA gene is part an operon contains fruT, predicted to encode Bacillus subtilis antiterminator LicT. Transcriptional showed that fru induced fructose. structure,...
The gene encoding the manganese-containing superoxide dismutase (MnSOD) of Enterococcus faecalis was characterized. It is transcribed monocistronically from an upstream promoter identified by rapid amplification cDNA ends (RACE)-PCR. A sodA mutant constructed and Growth strain not significantly different that its wild-type counterpart in standing aerated cultures. However, more sensitive towards menadione hydroperoxide stresses. response to H(2)O(2) stress analysed detail, mode killing this...
Background In many bacteria, the phosphotransferase system (PTS) is a key player in regulation of assimilation alternative carbon sources notably through catabolic repression. The intracellular pathogens Brucella spp. possess four PTS proteins (EINtr, NPr, EIIANtr and an EIIA mannose family) but no permease suggesting that this might serve only regulatory functions. Methodology/Principal Findings vitro biochemical analyses vivo detection two forms (phosphorylated or not) established...
The ability of Salmonella to cause disease depends on metabolic activities and virulence factors. Here, we show that a key protein, EIIAGlc, is absolutely essential for acute infection, but not survival, in mouse typhoid fever model. Surprisingly, phosphorylation-dependent EIIAGlc functions, including carbohydrate transport activation adenylate cyclase global regulation, do explain this phenotype. Instead, biochemical studies, vitro secretion translocation assays, vivo genetic epistasis...
Chronic infections caused by persistent pathogens represent an important health problem. Here, we establish a simple practical mouse Salmonella infection model for identifying bacterial maintenance functions that are essential persistency. In this model, substantial fraction of survived even several days treatment with potent fluoroquinolone antibiotic indicating stringency the model. Evaluation twelve metabolic defects revealed dramatically different requirements during persistency as...
A proteome analysis of Lactobacillus casei mutants that are affected in carbon catabolite repression revealed a 15-kDa protein was strongly overproduced ptsHI47T mutant. This identified as EIIA mannose class phosphotransferase system (PTS). 7.1-kb DNA fragment containing the EIIA-encoding open reading frame and five other genes sequenced. The first gene encodes resembling RpoN (sigma54)-dependent Bacillus subtilis transcription activator LevR. following pentacistronic operon is oriented...
When carrying out a proteome analysis with <i>ptsH3 </i>mutant of<i> Lactobacillus casei</i>, we found that the cold shock protein CspA was significantly overproduced compared to wild-type strain. We also noticed and CspB of <i>L. casei</i> CSPs from other organisms exhibit significant sequence similarity C-terminal part EIIA<sup>Glc</sup>, glucose-specific component phosphoenolpyruvate:sugar phosphotransferase system. This suggested direct...
The bacterial phosphotransferase system (PTS) transports and phosphorylates sugars, but also carries out numerous regulatory functions. β-proteobacterium Neisseria meningitidis possesses an incomplete PTS unable to transport carbon sources because it lacks a membrane component. Nevertheless, the residual phosphorylation cascade is functional meningococcal was therefore expected carry roles. Interestingly, ΔptsH mutant (lacks protein HPr) exhibited reduced virulence in mice after...
We identified the genes encoding proteins for transport of glucose and maltose in Neisseria meningitidis strain 2C4-3. A mutant deleted NMV_1892(glcP) no longer grew on deletion NMV_0424(malY) prevented utilization maltose. also purified characterized glucokinase α-phosphoglucomutase, which catalyze early catabolic steps two carbohydrates. N. catabolizes carbohydrates either via Entner-Doudoroff (ED) pathway or pentose phosphate pathway, thereby forming glyceraldehyde-3-P pyruvate...
Lactobacillus casei strains 64H and BL23, but not ATCC 334, are able to ferment D-ribitol (also called D-adonitol). However, a BL23-derived ptsI mutant lacking enzyme I of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) was utilize this pentitol, suggesting that strain BL23 transports phosphorylates via PTS. We identified an 11-kb region in genome sequence L. (LCABL_29160 LCABL_29270) which is absent from 334 contains genes for GlpR/IolR-like repressor, four components...