A5007697077- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Extracellular vesicles in disease
- Immune cells in cancer
- Neurogenesis and neuroplasticity mechanisms
- RNA modifications and cancer
- Cerebrospinal fluid and hydrocephalus
- Intracerebral and Subarachnoid Hemorrhage Research
- Dementia and Cognitive Impairment Research
- Nuclear Receptors and Signaling
- RNA Research and Splicing
- Multiple Sclerosis Research Studies
- Alzheimer's disease research and treatments
University of Eastern Finland
2017-2024
Max Delbrück Center
2024
University of Copenhagen
2015-2017
Sapienza University of Rome
2015
Here we elucidate the effect of Alzheimer disease (AD)-predisposing genetic backgrounds, APOE4, PSEN1ΔE9, and APPswe, on functionality human microglia-like cells (iMGLs). We present a physiologically relevant high-yield protocol for producing iMGLs from induced pluripotent stem cells. Differentiation is directed with small molecules through primitive erythromyeloid progenitors to re-create microglial ontogeny yolk sac. The express signature genes respond ADP intracellular Ca2+ release...
Abstract Hypoxia induces changes in the secretion of extracellular vesicles (EVs) several non‐neuronal cells and pathological conditions. EVs are packed with biomolecules, such as microRNA(miR)‐21‐5p, which respond to hypoxia. However, true EV association miR‐21‐5p, its functional or biomarker relevance, inadequately characterised. Neurons extremely sensitive cells, it is not known whether neuronal miR‐21‐5p altered upon Here, we characterised temporal profile cell viability neurons under...
Abstract Discovery of meningeal lymphatic vessels (LVs) in the dura mater, also known as dural LVs (dLVs) that depend on vascular endothelial growth factor C expression, has raised interest their possible involvement Alzheimer’s disease (AD). Here we find APdE9 and 5xFAD mouse models AD, amyloid-β (Aβ) is confined to blood dLV morphology or function not altered. The induction sustained atrophy hyperplasia AD mice by blocking overexpressing C, impaired improved, respectively, macromolecular...
miRandola (http://mirandola.iit.cnr.it/) is a database of extracellular non-coding RNAs (ncRNAs) that was initially published in 2012, foreseeing the relevance ncRNAs as non-invasive biomarkers. An increasing amount experimental evidence shows are frequently dysregulated diseases. Further, have been discovered different forms, such exosomes, which circulate human body fluids. Thus, 2017 an effort to update and collect accumulating information on spread across scientific publications...
Brain functionality relies on finely tuned regulation of gene expression by networks non-coding RNAs (ncRNAs) such as the one composed circular RNA ciRS-7 (also known CDR1as), microRNA miR-7, and long ncRNA Cyrano. We describe ischemia-induced alterations in network both vitro vivo transgenic mice lacking or miR-7. Our data show that cortical neurons downregulate Cyrano upregulate miR-7 during ischemia. Mice exhibit reduced lesion size motor impairment, while absence alone results increased...
Abstract Background Ischemic stroke is a devastating disease without cure. The available treatments for ischemic stroke, thrombolysis by tissue plasminogen activator, and thrombectomy are suitable only to fraction of patients thus novel therapeutic approaches urgently needed. neuroinflammatory responses elicited secondary the attack further aggravate stroke-induced neuronal damage. It has been demonstrated that these regulated at level non-coding RNAs, especially miRNAs. Methods We utilized...
Abstract INTRODUCTION Alzheimer's disease (AD) is a neurodegenerative and the main cause of dementia in elderly. AD pathology characterized by accumulation microglia around beta‐amyloid (Aβ) plaques which assumes disease‐specific transcriptional signatures, as for disease‐associated (DAM). However, regulators microglial phagocytosis are still unknown. METHODS We isolated Aβ‐laden from brain 5xFAD mice RNA sequencing to characterize signature phagocytic identify key non‐coding RNAs capable...
Abstract Ischemic stroke, the third leading cause of death in Western world, affects mainly elderly and is strongly associated with comorbid conditions such as atherosclerosis or diabetes, which are pathologically characterized by increased inflammation known to influence outcome stroke. Stroke incidence peaks during influenza seasons, patients suffering from infections pneumonia prior stroke exhibit a worse outcome. Earlier studies have shown that comorbidities aggravate yet mediators this...
Abstract Aberrant and sustained activation of microglia is implicated in the progression severity multiple sclerosis (MS). However, whether intrinsic alterations microglial function impact pathogenesis this disease remains unclear. We conducted transcriptomic functional analyses microglia-like cells (iMGLs) differentiated from induced pluripotent stem (iPSCs) patients with MS (pwMS) to answer question. generated iPSCs six pwMS showing increased activity via translocator protein (TSPO)-PET...
The central nervous system (CNS) has for long been considered to be devoid of proper lymphatic vascular system. However, recent studies suggest that meningeal vessels may take part into clearance waste products, macromolecules and immune cells systemic blood circulation. We set out investigate the contribution in drainage beta-amyloid (Aß) protein from brain hypothesized lack Alzheimer's disease (AD) transgenic mice would lead faster Aß accumulation. cross-bred amyloid plaque producing...
Over the last decade, development of new technologies for genome-wide analyses eukaryotic transcriptome has allowed comprehensive studies circRNA species. Although biologists identified circular transcripts more than 20 years ago, these molecules were long considered artefacts aberrant splicing reactions or prerogative a few viral pathogens. The evidence from expression data indicates wide range thousands endogenous circRNAs in mammalian cells, some which are abundant, conserved, and stably...
ABSTRACT Brain functionality relies on finely tuned regulation of gene expression by networks non-coding RNAs (ncRNAs) such as the one composed circular RNA ciRS-7 (also known CDR1as), microRNA miR-7 and long Cyrano. Here we describe ischemia induced alterations in ncRNA network both vitro vivo transgenic mice lacking or miR-7. Our data show that cortical neurons downregulate Cyrano upregulate upon ischemic insults. Mice reduced lesion size motor impairment, whilst absence alone leads to an...