A5042617683- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Congenital heart defects research
- RNA Research and Splicing
- Extracellular vesicles in disease
- Peptidase Inhibition and Analysis
- Tissue Engineering and Regenerative Medicine
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
University of Eastern Finland
2017-2024
Abstract Hypoxia induces changes in the secretion of extracellular vesicles (EVs) several non‐neuronal cells and pathological conditions. EVs are packed with biomolecules, such as microRNA(miR)‐21‐5p, which respond to hypoxia. However, true EV association miR‐21‐5p, its functional or biomarker relevance, inadequately characterised. Neurons extremely sensitive cells, it is not known whether neuronal miR‐21‐5p altered upon Here, we characterised temporal profile cell viability neurons under...
Brain functionality relies on finely tuned regulation of gene expression by networks non-coding RNAs (ncRNAs) such as the one composed circular RNA ciRS-7 (also known CDR1as), microRNA miR-7, and long ncRNA Cyrano. We describe ischemia-induced alterations in network both vitro vivo transgenic mice lacking or miR-7. Our data show that cortical neurons downregulate Cyrano upregulate miR-7 during ischemia. Mice exhibit reduced lesion size motor impairment, while absence alone results increased...
Changes in mature microRNA (miRNA) levels that occur downstream of signaling cascades play an important role during human development and disease. However, the regulation primary (pri-miRNA) genes remains to be dissected detail. To address this, we followed a data-driven approach developed transcript identification, validation quantification pipeline for characterizing regulatory domains pri-miRNAs. Integration 92 nascent transcriptomes multilevel data from cells arising ecto-, endo-...
Abstract Ischemic stroke, the third leading cause of death in Western world, affects mainly elderly and is strongly associated with comorbid conditions such as atherosclerosis or diabetes, which are pathologically characterized by increased inflammation known to influence outcome stroke. Stroke incidence peaks during influenza seasons, patients suffering from infections pneumonia prior stroke exhibit a worse outcome. Earlier studies have shown that comorbidities aggravate yet mediators this...
Pharmacological modulation of cell fate decisions and developmental gene regulatory networks holds promise for the treatment heart failure. Compounds that target tissue-specific transcription factors could overcome non-specific effects small molecules lead to regeneration muscle following myocardial infarction. Due cellular heterogeneity in heart, activation programs representing specific atrial ventricular cardiomyocyte subtypes would be highly desirable. Chemical compounds modulate used...
MicroRNAs are key players in gene regulatory networks controlling cell homeostasis. Their altered expression has been previously linked to disease outcomes and microRNAs thus serve as biomarkers for diagnostics. However, their synthesis its transcriptional regulation have challenging investigate. In this study, we validated the use of H3K36me3 histone modification quantification microRNA transcription levels using data from ENCODE Consortium then applied approach provide new insight into...
ABSTRACT MicroRNAs (miRNAs) are a class of regulatory non-coding RNAs that finetune cellular functions by modulating the stability and abundance their target mRNAs, thereby contributing to regulation tissue homeostasis. MiRNA genes transcribed similarly protein-coding recent studies have enabled annotation quantification genome-wide from bulk nascent transcriptomes. Here, we developed an approach quantify integrate miRNA gene signatures into single-cell studies. To characterize expression...
ABSTRACT Brain functionality relies on finely tuned regulation of gene expression by networks non-coding RNAs (ncRNAs) such as the one composed circular RNA ciRS-7 (also known CDR1as), microRNA miR-7 and long Cyrano. Here we describe ischemia induced alterations in ncRNA network both vitro vivo transgenic mice lacking or miR-7. Our data show that cortical neurons downregulate Cyrano upregulate upon ischemic insults. Mice reduced lesion size motor impairment, whilst absence alone leads to an...