A5003163815- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- HIV/AIDS drug development and treatment
- Immune Cell Function and Interaction
- Multiple Myeloma Research and Treatments
- CAR-T cell therapy research
- SARS-CoV-2 detection and testing
- CRISPR and Genetic Engineering
- Cell Adhesion Molecules Research
- Mosquito-borne diseases and control
- Bone health and osteoporosis research
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- Osteoarthritis Treatment and Mechanisms
- HIV/AIDS Research and Interventions
- Cancer Mechanisms and Therapy
- Glioma Diagnosis and Treatment
- Bacillus and Francisella bacterial research
- Extracellular vesicles in disease
- Chemokine receptors and signaling
- Quinazolinone synthesis and applications
- Peatlands and Wetlands Ecology
- Hair Growth and Disorders
- Lipid metabolism and disorders
- Advancements in Transdermal Drug Delivery
Fudan University
2017-2025
Huashan Hospital
2019-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2025
State Key Laboratory of Genetic Engineering
2017-2024
Donghua University
2024
Wuhan University
2020-2023
University at Buffalo, State University of New York
2016-2023
The Third Affiliated Hospital of Zhejiang Chinese Medical University
2023
Guizhou University of Finance and Economics
2022
University of Iowa
2019-2021
The massive and rapid transmission of SARS-CoV-2 has led to the emergence several viral variants concern (VOCs), with most recent one, B.1.1.529 (Omicron), which accumulated a large number spike mutations, raising specter that this newly identified variant may escape from currently available vaccines therapeutic antibodies. Using VSV-based pseudovirus, we found Omicron is markedly resistant neutralization sera convalescents or individuals vaccinated by two doses inactivated whole-virion...
In the current global crisis of antibiotic resistance, delivery systems are emerging to combat resistant bacteria in a more efficient manner. Despite significant advances nanocarriers, many challenges like poor biocompatibility, premature drug release, suboptimal targeting infection sites and short blood circulation time still challenging. To achieve targeted enhance antibacterial activity, here we reported kind pH-responsive nanoparticles by simply self-assembly an amphiphilic poly(ethylene...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes multi-organ damage, which includes hepatic dysfunction, as observed in over 50% of COVID-19 patients. Angiotensin I converting enzyme (peptidyl-dipeptidase A) (ACE2) is the primary receptor for SARS-CoV-2 entry into host cells, and studies have shown presence intracellular virus particles human hepatocytes that express ACE2, but at extremely low levels. Consequently, we asked if might receptors other than ACE2...
Diabetic foot ulcers, a common complication of diabetes mellitus, significantly impact patients' quality life and impose substantial economic burden on healthcare systems. However, the currently used treatments are associated with various challenges traditionally dressings lack functional efficacy. Oxidative stress is believed to play vital role in diabetic wound healing. Therefore, nicotinamide mononucleotide (NMN), which known for its antioxidant properties, offers potential accelerate...
Cells harboring latent HIV-1 pose a major obstacle to eradication of the virus. The 'shock and kill' strategy has been broadly explored purge reservoir; however, none current latency-reversing agents (LRAs) can safely effectively activate virus in patients. In this study, we report an ingenol derivative called EK-16A, isolated from traditional Chinese medicinal herb Euphorbia kansui, which displays great potential reactivating HIV-1. A comparison doses used measure potency indicated EK-16A...
Abstract Background Treatment failures in cancers, including multiple myeloma (MM), are most likely due to the persistence of a minor population tumor-initiating cells (TICs), which noncycling or slowly cycling and very drug resistant. Methods Gene expression profiling real-time quantitative reverse transcription polymerase chain reaction were employed define genes differentially expressed between side-population cells, contain TICs, main MM derived from 11 patient samples. Self-renewal...
To explore the clinical efficacy of acupressure therapy combined with Tuina and active functional exercise in treatment neck-type cervical spondylosis. Sixty patients spondylosis were randomly divided into a control group an experimental group, 30 cases each group. The was treated exercise. lasted 10-15 min per session, twice week for 4 weeks. Functional exercises performed once morning evening, session lasting about 10 min, total received same as ringheaded thumb-tack needle which applied...
Objective— Recent studies suggest that the E-selectin ligands expressed on human leukocytes may differ from those in other species, particularly mice. To elaborate this, we evaluated impact of glycosphingolipids myeloid cells regulating E-selectin-mediated cell adhesion. Approach and Results— A series modified lines primary neutrophils were created by targeting UDP-Glucose Ceramide Glucosyltransferase using either lentivirus-delivered shRNA or CRISPR-Cas9-based genome editing. Enzymology...
Article14 September 2020Open Access Source DataTransparent process PEBP1 suppresses HIV transcription and induces latency by inactivating MAPK/NF-κB signaling Xinyi Yang State Key Laboratory of Genetic Engineering Research Center Gene Technology, Ministry Education, Institute Genetics, School Life Sciences, Fudan University, Shanghai, China Search for more papers this author Yanan Wang Panpan Lu Yinzhong Shen Department Infectious Disease, Medical Molecular Virology Education/Health, Basic...
HIV-specific chimeric antigen receptor (CAR) T-cells have been developed to target HIV-1 infected CD4+ that express HIV Env proteins. However, T cell exhaustion and the patient-specific autologous paradigm of CAR-T hurdled clinical applications. Here, we created cells using human peripheral blood mononuclear a 3BNC117-E27 (3BE) CAR construct enabled expression programmed death protein (PD-1) -blocking scFv E27 single-chain variable fragment HIV-1-specific broadly neutralizing antibody...
Background Falcine sinuses can remain persistent after birth, but they also become recanalized in cases where venous are invaded by meningiomas. Purpose To explore the incidence and imaging features of falcine healthy individuals parasagittal meningioma (PSM) patients on magnetic resonance venography (MRV). Material Methods Radiologists evaluated data 168 PSM patients. The invasion extent superior sagittal sinus (SSS) PSMs was confirmed a neurosurgeon based Sindou's criteria. We compared...
Acquired immunodeficiency syndrome (AIDS) cannot be completely cured, and the main obstacle is existence of viral reservoirs. However, we currently do not fully understand molecular mechanisms by which HIV-1 latency established maintained. Here, based on engineered chromatin immunoprecipitation (enChIP) technology that using FLAG-tagged zinc finger nucleic acid proteins (FLAG-ZFP) bind to L region immunoprecipitation, identified RYBP as a new latency-promoting gene. The effect was explored...
To investigate the mechanism of minoxidil in treating androgenetic alopecia (AGA).The action on AGA was first systematically investigated from viewpoint network pharmacology, including minoxidil-AGA target prediction, protein-protein interaction (PPI) analysis, molecular docking and enrichment analysis targets related to AGA, dermal papilla cell assays confirm viability prediction.The combined revealed that treatment not only acts androgenic receptors (AR) but also 2 new targets, steroid...
The increasing concern over climate change has spurred significant interest in exploring the potential of microalgae for wastewater treatment. Among various types industrial wastewaters, high-salinity NH4+-N stands out as a common challenge. Investigating microalgae’s resilience to under conditions and their efficacy utilization is crucial advancing treatment technologies. This study evaluated effectiveness employing nitrogen-efficient microalgae, specifically Oocystis lacustris, removal...
MAPKAPK2 (MK2), the direct substrate of p38 MAPK, has been well-acknowledged as an attractive drug target for cancer therapy. However, few studies have assessed functions it in multiple myeloma (MM). In present study, MK2 expression MM patients was analyzed by gene profiling (GEP) and array-based comparative genomic hybridization (aCGH). Several experiments vitro including MTT assay, Western blot flow cytometry analysis were performed to identify function MM. addition, we conducted mouse...
Chimeric antigen receptor (CAR)-T cells, a therapeutic agent for solid tumors, are not completely effective due to lack of infiltration T cells into the tumor site and immunity caused by Programmed Death Receptor 1(PD1). Here, an epidermal growth factor (EGFR) CAR-T cell was engineered express chemokine CCR6 secrete PD1 blocking Single-chain antibody fragment (scFv) E27 enhance their anti-tumor effects. The findings showed that enhanced migration EGFR CAR-E27-CCR6 in vitro Transwell assay....
The discovery of artemisinin (ART) for malaria treatment won the 2015 Nobel Prize in Medicine, which inspired rediscovery and development ART other diseases including cancer. In this study, we investigated potential therapeutic effect dihydroartemisinin (DHA) on multiple myeloma (MM) cells primary MM 5TMM3VT mouse model. Both
Lidocaine, a potent local anesthetic, is clinically used in nerve block and pain management. However, due to its short half-life, repeated administration required. For this reason, here we designed prepared lidocaine-encapsulated polylactic acid-glycolic acid (Lidocaine@PLGA) microcapsule with ultrasound responsiveness relieve the sciatica pain. With premixed membrane emulsification strategy, fabricated lidocaine-embedded microcapsules possessed uniform particle size, good stability,...