- Fungal Biology and Applications
- Phytochemicals and Antioxidant Activities
- Polysaccharides and Plant Cell Walls
- melanin and skin pigmentation
- Pharmacological Effects of Natural Compounds
- Fermentation and Sensory Analysis
- Antioxidant Activity and Oxidative Stress
- Steroid Chemistry and Biochemistry
- Natural Antidiabetic Agents Studies
- Phytochemistry and Bioactivity Studies
- Mycorrhizal Fungi and Plant Interactions
- Cholesterol and Lipid Metabolism
- Meat and Animal Product Quality
- Plant biochemistry and biosynthesis
- Free Radicals and Antioxidants
- Essential Oils and Antimicrobial Activity
- Microbial Metabolites in Food Biotechnology
- Enzyme-mediated dye degradation
- Protein Hydrolysis and Bioactive Peptides
- Fatty Acid Research and Health
- Biochemical Analysis and Sensing Techniques
- Edible Oils Quality and Analysis
- Phytochemical compounds biological activities
- Vitamin D Research Studies
- Hormonal and reproductive studies
Instituto de Investigación en Ciencias de la Alimentación
2015-2024
Universidad Autónoma de Madrid
2015-2024
Centro de Investigación y Tecnología Agroalimentaria de Aragón
2019
Consejo Superior de Investigaciones Científicas
2014
IMDEA Food
2011
Centro de Edafología y Biología Aplicada del Segura
2000-2001
Instituto de Agroquímica y Tecnología de Alimentos
2000
Wageningen University & Research
2000
The total free radical scavenger capacity (RSC) of 57 edible oils from different sources was studied: olive (24 brands oils), sunflower (6), safflower (2), rapeseed (3), soybean linseed corn hazelnut walnut sesame almond mixture for salad "dietetic" oil and peanut (2). Olive were also studied according to their geographical origins (France, Greece, Italy, Morocco, Spain, Turkey). RSC determined spectrophotometrically by measuring the disappearance 2,2-diphenyl-1-picrylhydrazyl (DPPH•) at 515...
In this paper, oleuropein and some other related phenolic compounds are reviewed. Their occurrence, distribution, biosynthesis transformation during maturation industrial processing (preparation of table olives oil production) described. role in human health is proposed based on current human, animal vitro studies as molecules with antioxidant antimicrobial properties. © 2000 Society Chemical Industry
The antiradical capacity (radical scavenger capacity, RSC) of anthocyanin-based fruit extracts prepared in the laboratory (black chokeberry, black-thorn, and strawberry) was studied by using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•). To determine their RSC, second-order rate constant (k2) for oxidation these DPPH• calculated. value k2 compared to that used food industry as natural (α-tocopherol) or synthetic (butylated hydroxytoluene (BHT) butylated hydroxyanisole (BHA)) antioxidants,...
An easy and fast test has been designed to compare the total free radical scavenging capacity (RSC) of various food samples. Black green teas from different countries, wines brands were studied compared as examples coloured liquids (water- methanol-soluble); oils sources used lipidic foodstuffs; apples varieties spinach analysed solid foods. Dilutions extracts described foodstuffs prepared aliquots each dilution spotted onto TLC silica gel layers in form a dot-blot test: stained with...
Microwave-assisted extraction (MAE) and pressurized liquid (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus Ganoderma lucidum fruiting bodies. Extraction effectiveness was by a full-factorial experimental design (response surface methodology, RSM), using water solvent. Total carbohydrate content of the obtained extracts polysaccharide yields variable responses investigated, while temperature time...
A polysaccharide-enriched extract obtained from Lentinula edodes was submitted to several purification steps separate three different D-glucans with β-(1→6), β-(1→3),(1→6) and α-(1→3) linkages, being characterized through GC-MS, FT-IR, NMR, SEC colorimetric/fluorimetric determinations. Moreover, in vitro hypocholesterolemic, antitumoral, anti-inflammatory antioxidant activities were also tested. Isolated glucans exerted HMGCR inhibitory activity, but only β-(1→6) fractions showed DPPH...
Hydroxytyrosol (HTyr), a natural ortho-diphenolic antioxidant with health-beneficial properties that mainly occurs in virgin olive oil and mill waste waters (also known as vegetative waters), has been enzymatically synthesized using mushroom tyrosinase. This o-diphenol (not commercially available) was obtained from its monophenolic precursor tyrosol (commercially the presence of both tyrosinase ascorbic acid. The reaction synthesis is continuous, easy to perform, adaptable bioreactor for...
Abstract A new procedure has been developed to separate and characterize antioxidant compounds from Spirulina platensis microalga based on the combination of pressurized liquid extraction (PLE) different chromatographic procedures, such as TLC, at preparative scale, HPLC with a diode array detector (DAD). Different solvents were tested for PLE antioxidants S. microalga. An optimized process using ethanol (generally recognized safe, GRAS) solvent obtained that provides natural extracts high...
Abstract Edible mushrooms are a good source of antioxidants. Methanol extracts such as Pleurotus sp., Agaricus bisporus, Morchella esculenta, Boletus edulis (approx. 2 mg mL −1 ) showed high 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) scavenging activity close to 90%. Water even higher antioxidant activity. In this case, B. edulis, Lentinus edodes and Amanita cesarea the highest 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) at approx. 0.14 . Other Lactarius deliciosus Cantharellus...
A pressurized water extraction (PWE) method was developed in order to extract β-glucans with bile acids-binding capacities from cultivated mushrooms (Agaricus bisporus, Lentinula edodes, and Pleurotus ostreatus) be used as supplements design novel foods hypocholesterolemic properties. Extraction yields were higher individual than sequential extractions being the optimal parameters: 200°C, 5 cycles of min each at 10.3 MPa. The crude polysaccharide (PSC) fractions, isolated PWE extracts...
Three extraction methods were sequentially combined to obtain fractions from Lentinula edodes (shiitake mushrooms) containing bioactive compounds against cardiovascular diseases (CVDs). Fruiting bodies first extracted with plain water, obtained residue was then submitted supercritical fluid (SFE) and remaining hot water extraction. Sequential design allowed reutilization of the nonextracted material as raw for successive extractions increasing yields separating interesting compounds....
Mushrooms are a source of dietary fiber (DF) with cholesterol-lowering effect. However, their underlying mechanisms poorly understood. The effect DF-enriched fractions from three mushrooms species on cholesterol-related expression was studied in vitro. Pleurotus ostreatus DF fraction (PDF) used mice models to assess its potential palliative or preventive against hypercholesterolemia. PDF induced transcriptional response Caco-2 cells, suggesting possible In the setting, reduced hepatic...