Human respiratory infections caused by coronaviruses can range from mild to deadly. Although there are numerous studies on coronavirus disease 2019 (COVID-19), few have been published its Omicron variant. In order remedy this deficiency, study undertook a bibliometric analysis of the publishing patterns variant and identified hotspots. Automated transportation, environmental protection, improved healthcare, innovation in banking, smart homes just areas where machine learning has found use...

Colonization of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae as animal gut microbiota is a substantial global threat. This study aimed to determine the molecular characterization bla SHV , TEM and CTX-M variants in animals, well evaluate antimicrobial resistance conferred by these genes. We prospectively analyzed 1273 fecal specimens farm domestic animals for isolation enterobacteria that had ESBL phenotype using biochemical methods. The extracted genes were amplified...

Clinical image processing plays a significant role in healthcare systems and is widely used methodology of the current era. The Intracranial tumor affects children adults as it 10th most common form tumor. Accurate segmentation brain complicated even though images are acquired properly. can be curable if its stage identified on time, for this purpose, researchers have been developing sophisticated techniques methods. An automatic detection, segmentation, colorization, classification region...

Antibiotic-resistant bacteria causing foodborne serious illnesses can be found in contaminated food. Therefore, this study aimed to identify the pathogens, genes, and antimicrobial residues present raw milk meat. We collected 40 beef samples using aseptic method from various parts of Faisalabad metropolis, Pakistan. The were cultured on blood, MacConkey, UTI chrome agar. VITEK 2 compact system was used for microbial identification determination minimum inhibitory concentrations....

The pH-sensitive polymeric matrix of basil seed gum (BSG), with two different monomers, such as acrylic acid (AA) and N, N-Methylene-bis-acrylamide (MBA), was selected to use in hydrogels preparation through a free radical copolymerization technique using potassium per sulfate (KPS) cross linker. BSG, AA MBA were used multiple ratios investigate the polymer, monomer initiator effects on swelling properties release pattern captopril. Characterization formulated done by FTIR, DSC/TGA, XRD SEM...

COVID-19 is a pandemic that has affected nearly every country in the world. At present, sustainable development area of public health considered vital to securing promising and prosperous future for humans. However, widespread diseases, such as COVID-19, create numerous challenges this goal, some those are not yet defined. In study, Shallow Single-Layer Perceptron Neural Network (SSLPNN) Gaussian Process Regression (GPR) model were used classification prediction confirmed cases five...

Vitamin D status is a key determinant of maternal and neonatal health. Deficiency has been reported to be common in Pakistani women, but information regarding environmental genetic determinants vitamin lacking this population.We conducted cross-sectional study among three groups healthy women living Lahore, Pakistan: university students, students or employees Medrasas Islamic Institutes, working office, hospital domestic settings. Multivariate analysis was performed identify status:...

Plasmid-mediated colistin resistance (Col-R) conferred by mcr genes endangers the last therapeutic option for multifarious β-lactamase-producing bacteria. The current study aimed to explore gene molecular epidemiology in extensively drug-resistant (XDR) Col-R gram-negative bacterial strains were screened using a minimum inhibitory concentration (MIC) breakpoint ≥4 µg/mL. Resistant isolates examined variants, extended-spectrum β-lactamase, AmpC, and carbapenemase polymerase chain reaction...

Single nucleotide polymorphisms (SNPs) in the genes encoding vitamin D receptor (VDR) and binding protein (DBP) have been reported to modify influence of deficiency on susceptibility active tuberculosis (TB) UK, but this phenomenon has not investigated settings with a high TB burden. SNPs CYP2R1, which encodes 25-hydroxylase enzyme, are known status, their potential role determining previously any setting. We conducted case–control study 260 pulmonary patients 112 controls recruited Lahore,...

Abstract Background The global emergence of plasmid-mediated colistin resistance (Col-R) conferred by mcr genes in gram-negative rods (GNRs) has jeopardized the last treatment option for multidrug-resistant bacterial infections humans. This study aimed to assess gene-mediated Col-R GNRs isolated from humans and animals Pakistan. Methods Animal clinical specimens collected various sources were prospectively analysed using standard microbiological procedures. Pathogens identified API 20E 20NE...

The human microbiome is a complex ecosystem of bacteria residing in the body. It plays crucial role safeguarding overall well-being individuals while also making them more vulnerable to various diseases. microbiota, genetics, and health have connection, which significant for maintaining preventing infections. has several gastrointestinal, skin, dental, systemic diseases, such as obesity, diabetes, autoimmune disorders. Genetic variation lifestyle dietary choices modify composition, thereby...

Vitamin D, a fat soluble vitamin, is well known for calcium homeostasis. In recent years, many researchers have suggested that vitamin D performs multiple functions extending far beyond mineral The synthesis machinery and related receptor been reported in tissues, where they play key role immune system modulation. Deficiency of not linked only with rickets or osteomalacia but other infectious metabolic disorders. Emerging evidences suggest the relation deficiency establishing tuberculosis....

Mimosa pudica seed mucilage (MPM) is composed of glucuronoxylan, which a swellable, pH-responsive and non-toxic biomaterial. Herein, we aimed to extract MPM from M. seeds (MP seeds) ascertain optimization extraction conditions get highest yield by response surface methodology, via Box-Behnken design (RSM-BBD). was extracted MP hot water method. The effects four different parameters on the were evaluated: pH medium (1-10), seed/water contact time (1-12 h), temperature (30-90 °C), ratio...

The therapeutic management of carbapenem-resistant Acinetobacter baumannii (CR-AB) represents a serious challenge to the public health sector because these pathogens are resistant wide range antibiotics, resulting in limited treatment options. present study was planned investigate clonal spread CR-AB clinical setting.A total 174 A. isolates were collected from tertiary care hospitals Lahore, Pakistan. confirmed by VITEK 2 compact system and molecular identification recA blaOXA-51....

Extensively drug-resistant (XDR) Salmonella enterica serovar Typhi (S. Typhi) poses a grave threat to public health due increased mortality and morbidity caused by typhoid fever. Honey is promising antibacterial agent, we aimed determine the activity of honey against XDR S. Typhi.We isolated 20 clinical isolates from pediatric septicemic patients determined minimum inhibitory concentrations (MICs) different antibiotics pathogens using VITEK 2 Compact system. Antimicrobial-resistant genes...

Emerging drug resistance and hindrance of treatment is provoking scientists to search new, less expensive medicinally active compounds. Dental diseases caused by oral pathogens are very frequent chronic infections around the world. The medical potentials a lot Pakistani local herbs herbal combinations relatively unknown, hence attempted explore. A study was designed investigate potential role medicinal for example Miswak, Kalonji & Aloe vera as antimicrobial, antioxidant anti-proliferative...

Background The emergence of the New Dehli metallo-beta-lactamase (NDM) gene in Enterobacteriaceae is responsible for multidrug resistance severe infections and serious morbidity patients. Our study aimed to define molecular characteristics antibiogram NDM-1 producing Enterobacteriaceae. Methods We isolated 370 individual enterobacteria from clinical specimens collected two tertiary hospitals Sakaka, Saudi Arabia. Bacterial isolation was performed using standard microbiological techniques...

This study aimed to find out the prevalence and antimicrobial resistance profile of Klebsiella pneumoniae in raw food items. A total 261 items, including vegetables, fruits, meat, milk samples, were collected processed for isolation K. pneumoniae. Further susceptibility testing molecular analysis was done analyze drug encoding genes. The rate found be high (38%), samples predominantly contaminated (19/51), followed by fruits (12/51), meat (11/51), vegetables (9/51). However, no significant...

Foodborne pathogens have acquired the ability to produce biofilms survive in hostile environments. This study evaluated biofilm formation, antimicrobial resistance (AMR), and heavy metal tolerance of bacteria isolated from dairy non-dairy food products. We aseptically collected processed 200 specimens peptone broth, incubated them overnight at 37 °C, sub-cultured on various culture media. Bacterial growth was identified with biochemical tests API 20E 20NE strips. The AMR isolates observed...