A5003262635- Viral Infections and Vectors
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Virology and Viral Diseases
- Vector-Borne Animal Diseases
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Tuberculosis Research and Epidemiology
- Cancer therapeutics and mechanisms
- Mycobacterium research and diagnosis
- Bacillus and Francisella bacterial research
- COVID-19 diagnosis using AI
- COVID-19 Clinical Research Studies
- HIV Research and Treatment
- Microbial infections and disease research
- Malaria Research and Control
- Hepatitis C virus research
- Surgical Sutures and Adhesives
- HIV/AIDS drug development and treatment
- vaccines and immunoinformatics approaches
- Nail Diseases and Treatments
- Dermatologic Treatments and Research
University of Veterinary and Animal Sciences
2015-2024
Czech Academy of Sciences, Institute of Microbiology
2023
Fatima Jinnah Women University
2022
University of Health Sciences Lahore
2017-2021
Institute of Public Health Bengaluru
2020
Government of Pakistan
2018-2020
Government of Punjab
2018-2020
Primary Health Care
2018
University of Lahore
2017
Genetic changes in avian influenza viruses influence their infectivity, virulence and transmission. Recently we identified a novel genotype of H9N2 widespread circulation poultry Pakistan that contained polymerases (PB2, PB1 PA) non-structural (NS) gene segments identical to highly pathogenic H7N3 viruses. Here, investigated the potential these cause disease assessed transmission capability virus within between wild terrestrial species. Groups broilers, layers, jungle fowl, quail, sparrows...
One year after a virulent Newcastle disease virus (vNDV) outbreak in Pakistan, the causative strain was present vaccinated chickens of multiple farms despite existence high-average NDV-specific antibody titers (>4.75 log2). The data suggest possible role birds as reservoirs vNDV.
Bacillus anthracis, the causative agent of anthrax, is a highly virulent zoonotic pathogen primarily affecting domesticated and wild herbivores. Human exposure to B. anthracis through contact with infected animals or contaminated animal products. In Pakistan, where livestock vaccines are largely unavailable carcasses often disposed improperly, risk humans, wildlife significant. Currently, diagnosis anthrax infections outbreak tracing necessitates isolation culturing process that requires...
Rabies is an infectious but preventable disease with proper vaccination. Pre-exposure prophylaxis means using medications or vaccines against a given pathogen in people who have not yet been exposed to the pathogen. Post-exposure use of are certain This study aimed evaluate seroconversion anti-rabies IgG antibodies following administration Indigenous rabies vaccine. For this purpose, n=150 individuals were enrolled 20% pre-exposure group (PrEPG) and 80% post-exposure (PEPG). The PEPG further...
There is a substantial relationship between gut microbiota and Autism Spectrum Disorder (ASD). Studies have found that there are significant changes in microbial composition people with ASD, their unaffected siblings, healthy controls. Though results still unstable, bacterial species like Clostridium, Sutterella, Desulfovibrio, Lactobacillus, Bacterodies, Faecalibacterium more common ASD patients. Because up to 90% of (ASD) experience gastrointestinal (GI) problems, it thought the gut-brain...
Newcastle disease (ND) is one of the most deadly diseases poultry around globe. The endemic in Pakistan and recurrent outbreaks are being reported regularly wild captive, rural commercial flocks. Though, efforts have been made to characterize causative agent some parts country, genetic nature strains circulating throughout currently lacking. To ascertain genetics NDV, 452 blood samples were collected from 113 flocks, originating all provinces Pakistan, showing high mortality (30–80%)....
Abstract Background Few serological tests are available for detecting antibodies against Mycoplasma capricolum subsp. capripneumoniae , the causal agent of contagious caprine pleuropneumonia (CCPP). The complement fixation test, test prescribed international trade purposes, uses a crude antigen that cross-reacts with all other mycoplasma species “mycoides cluster” frequently infecting goat herds. lack more specific has been real obstacle to evaluation prevalence and economic impact CCPP...
Approximately 170 million people are infected with Hepatitis C virus (HCV) worldwide. The prevalence of chronic HCV infections in Pakistan is about 5%, most individuals being genotype 3a. Data on genotypes distribution across various districts the country scarce. One example district Mardan from where such data available only 17 individuals. Accordingly, present study aimed at determining among 177 RNA positive Mardan.Serum samples (n = 215) patients suspected hepatitis were collected and...
Abstract Avian influenza A(H9N2) virus isolated from a poultry worker in Pakistan 2015 was closely related to viruses detected farms. Observed mutations the hemagglutinin receptor-binding affinity and antigenicity could affect cross-reactivity with prepandemic H9N2 vaccine strains.
Abstract Background Fluoroquinolones (FQs) are potential drugs that inhibit DNA synthesis and used in the treatment of multidrug-resistant tuberculosis (TB) short-term anti-TB regimens. In recent years, a high proportion FQ resistance has been observed Mycobacterium isolates. The development TB negatively impacts patient outcome is serious threat to control TB. Methods study included total 562 samples from patients with pulmonary had on anti-tuberculosis therapy. MTBDR sl assays were...
Avian paramyxovirus serotype 1 (APMV-1) was isolated from an acute and highly contagious outbreak in peacocks (Pavo cristatus) a wildlife park Pakistan. A velogenic neurotropic form of APMV-1 caused 100% case fatality rate killed 190 within week. Biological serological characterizations showed features strain APMV-1, these results were further confirmed by sequence analysis the cleavage site fusion protein. The complete genome one isolates sequenced, phylogenetic conducted. that this isolate...
Surveillance of H9N2 is currently focused on areas central to the commercial poultry industry. This study determined prevalence virus in and backyard flocks Punjab Province, Pakistan. Oral tracheal swabs were collected from January 2015 through June 2016. Antisera against H5, H7, H9, Newcastle disease viruses used for identification. Molecular confirmation was made by reverse transcription PCR. Avian influenza subtypes H5 H7 not detected. The isolated 5.7% 905 tested (5-10 birds/flock)....
ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections has affected more than 15 million people and, as of 22 July 2019, caused deaths 0.6 individuals globally. With the excretion SARS-CoV-2 in stool symptomatic and asymptomatic COVID-19 patients, its genome detection sewage water can be used a powerful epidemiological tool to predict number positive cases population. This study was conducted detect during lockdown. Sewage samples, from 28 pre-selected sites, were...
Nota de investigación- Mapeo marcadores genéticos asociados con la antigenicidad y el rango huéspedes en los virus influenza tipo A subtipo H9N2 que infectan a avicultura Pakistán. El objetivo del presente estudio fue mapear diversidad genética glicoproteína hemaglutinina (HA) (IAV) H9N2. Se aislaron veinticinco pollos engorde marzo julio 2019. amplificó gene HA se realizó un análisis filogenético para determinar relación evolutiva. compararon importantes residuos aminoácidos antigénicos...
Emerging drug resistance and hindrance of treatment is provoking scientists to search new, less expensive medicinally active compounds. Dental diseases caused by oral pathogens are very frequent chronic infections around the world. The medical potentials a lot Pakistani local herbs herbal combinations relatively unknown, hence attempted explore. A study was designed investigate potential role medicinal for example Miswak, Kalonji & Aloe vera as antimicrobial, antioxidant anti-proliferative...
The human pathogen Acinetobacter baumannii has emerged as a frequent cause of hospital-acquired infections, but infection animals rarely been observed. Here we analyzed an outbreak epidemic pneumonia killing hundreds sheep on farm in Pakistan and identified A. the infecting agent. A pure culture strain AbPK1 isolated from lungs sick was inoculated into healthy sheep, which subsequently developed similar disease symptoms. Bacteria re-isolated infected were shown to be identical inoculum,...
Bluetongue (BT), caused by bluetongue virus (BTV), is a vector-borne disease of small ruminants that has the potential to spread across international borders. Despite large populations susceptible animals and borders with BTV endemic countries, little known burden prevalent serotypes in province Balochistan Pakistan. We conducted cross-sectional study determine seroconversion selected districts using competitive enzyme-linked immunosorbent assay (cELISA) real-time polymerase chain reaction...
The highly pathogenic (HPAI) avian influenza A(H5N1) viruses have undergone reassortment with multiple non-N1-subtype neuraminidase genes since 2008, leading to the emergence of H5Nx viruses. established themselves quickly in birds and disseminated from China Africa, Middle East, Europe North America. Multiple genetic clades successively evolved through frequent mutations reassortment, posing a continuous threat domestic poultry causing substantial economic losses. Live bird markets are...
Background: The study was conducted in the various districts of Punjab, Pakistan, to ascertain incidence and epidemiology SARS-CoV-2 variants circulating population during fifth wave COVID-19.Method: A total 9603 nasopharyngeal swab samples suspected patients were collected from different Punjab December 2021 April 2022. In BSL-3 facility, an auto-extractor (Uni-medica) used extract RNA genome. Viral detection quantification performed using real-time reverse transcriptase PCR. Multiplex PCR...
Abstract The recent outbreaks of highly pathogenic avian influenza A(H5N1) virus in North and South America, including widespread infection cattle the United States, calls for an urgent assessment host range A viruses, particularly subtypes pandemic concern. We conducted a serological survey binding antibodies to B viruses goats (n=452) sheep (n=329) Pakistan found high seropositive rates hemagglutinin (HA) (AIV) H5 (23.9–34.0%), H7 (13.9– 37.1%), H9 (17.0–34.7%). In contrast, there were low...