A5070974511- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virology and Viral Diseases
- Vector-Borne Animal Diseases
- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Microbial infections and disease research
- SARS-CoV-2 detection and testing
- Probiotics and Fermented Foods
- Milk Quality and Mastitis in Dairy Cows
- Bacillus and Francisella bacterial research
- COVID-19 Clinical Research Studies
- vaccines and immunoinformatics approaches
- Mosquito-borne diseases and control
- Livestock and Poultry Management
- Mycobacterium research and diagnosis
- Gut microbiota and health
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- Tuberculosis Research and Epidemiology
- Genetic and phenotypic traits in livestock
- Hepatitis C virus research
- Fire effects on ecosystems
University of Veterinary and Animal Sciences
2016-2025
Czech Academy of Sciences, Institute of Microbiology
2023
The Pirbright Institute
2018
The Francis Crick Institute
2018
Fondation Mérieux
2018
Primary Health Care
2018
The impact of avian influenza caused by H9N2 viruses in Pakistan is now significantly more severe than previous years. Since all gene segments contribute towards the virulence virus, it was imperative to investigate molecular features and genetic relationships prevalent this region. Analysis sequences eight RNA from 12 isolated between 2005 2008 undertaken. hemagglutinin (HA) isolates were closely related Iran 2004 2007 contained leucine instead glutamine at position 226 receptor binding...
Genetic changes in avian influenza viruses influence their infectivity, virulence and transmission. Recently we identified a novel genotype of H9N2 widespread circulation poultry Pakistan that contained polymerases (PB2, PB1 PA) non-structural (NS) gene segments identical to highly pathogenic H7N3 viruses. Here, investigated the potential these cause disease assessed transmission capability virus within between wild terrestrial species. Groups broilers, layers, jungle fowl, quail, sparrows...
One year after a virulent Newcastle disease virus (vNDV) outbreak in Pakistan, the causative strain was present vaccinated chickens of multiple farms despite existence high-average NDV-specific antibody titers (>4.75 log2). The data suggest possible role birds as reservoirs vNDV.
Abstract Background Diarrhea poses a major threat to bovine calves leading mortality and economic losses. Among the causes of calf diarrhea, rotavirus is etiological agent may result in dysbiosis gut microbiota. The current study was designed investigate effect probiotic Limosilactobacillus fermentum (Accession No.OR504458) on microbial composition rotavirus-infected using 16S metagenomic analysis technique. Screening infection below one month age done through clinical signs Reverse...
Virulent viruses of the panzootic Avian avulavirus 1 (AAvV-1) sub-genotype VIIi were repeatedly isolated (2011-2016) from commercial chickens and multiple non-poultry avian species in Pakistan. These findings provide evidence for existence epidemiological links between Newcastle disease outbreaks poultry infections with virulent AAvV-1 strains other kept proximity to poultry. Our results suggest that endemicity Pakistan involves hosts environments.
Abstract We characterized 55 influenza A(H9N2) viruses isolated in Pakistan during 2014–2016 and found that the hemagglutinin gene is of G1 lineage internal genes have differentiated into a variety novel genotypes. Some isolates had up to 4-fold reduction hemagglutination inhibition titers compared with older viruses. Viruses A180T/V substitutions conveyed this antigenic diversity also caused 3,500-fold greater binding avian-like >20-fold human-like sialic acid receptor analogs. This...
We detected Crimean-Congo hemorrhagic fever virus infections in 4 provinces of Pakistan during 2017-2018. Overall, seroprevalence was 2.7% humans and 36.2% domestic livestock. Antibody prevalence highest rural areas, where increased contact with animals is likely.
Bacillus anthracis, the causative agent of anthrax, is a highly virulent zoonotic pathogen primarily affecting domesticated and wild herbivores. Human exposure to B. anthracis through contact with infected animals or contaminated animal products. In Pakistan, where livestock vaccines are largely unavailable carcasses often disposed improperly, risk humans, wildlife significant. Currently, diagnosis anthrax infections outbreak tracing necessitates isolation culturing process that requires...
Rabies is an infectious but preventable disease with proper vaccination. Pre-exposure prophylaxis means using medications or vaccines against a given pathogen in people who have not yet been exposed to the pathogen. Post-exposure use of are certain This study aimed evaluate seroconversion anti-rabies IgG antibodies following administration Indigenous rabies vaccine. For this purpose, n=150 individuals were enrolled 20% pre-exposure group (PrEPG) and 80% post-exposure (PEPG). The PEPG further...
Bovine mastitis is a common udder disease in cattle, mainly caused by bacteria and other infectious agents. Traditionally antibiotics are used for their treatment, but the development of antibiotic resistance has increased importance using non alternative such as probiotic. In current study previously vitro characterized isolate Lactobacillus plantarum CM49 infused into two groups cattle suffering from clinical (n = 5) sub-clinical 5). The bacterial composition diversity analysis milk...
There is a substantial relationship between gut microbiota and Autism Spectrum Disorder (ASD). Studies have found that there are significant changes in microbial composition people with ASD, their unaffected siblings, healthy controls. Though results still unstable, bacterial species like Clostridium, Sutterella, Desulfovibrio, Lactobacillus, Bacterodies, Faecalibacterium more common ASD patients. Because up to 90% of (ASD) experience gastrointestinal (GI) problems, it thought the gut-brain...
Newcastle disease (ND) is one of the most deadly diseases poultry around globe. The endemic in Pakistan and recurrent outbreaks are being reported regularly wild captive, rural commercial flocks. Though, efforts have been made to characterize causative agent some parts country, genetic nature strains circulating throughout currently lacking. To ascertain genetics NDV, 452 blood samples were collected from 113 flocks, originating all provinces Pakistan, showing high mortality (30–80%)....
Abstract Background Few serological tests are available for detecting antibodies against Mycoplasma capricolum subsp. capripneumoniae , the causal agent of contagious caprine pleuropneumonia (CCPP). The complement fixation test, test prescribed international trade purposes, uses a crude antigen that cross-reacts with all other mycoplasma species “mycoides cluster” frequently infecting goat herds. lack more specific has been real obstacle to evaluation prevalence and economic impact CCPP...
Approximately 170 million people are infected with Hepatitis C virus (HCV) worldwide. The prevalence of chronic HCV infections in Pakistan is about 5%, most individuals being genotype 3a. Data on genotypes distribution across various districts the country scarce. One example district Mardan from where such data available only 17 individuals. Accordingly, present study aimed at determining among 177 RNA positive Mardan.Serum samples (n = 215) patients suspected hepatitis were collected and...
Coxiella burnetii causes query (Q) fever, an important zoonotic disease with worldwide significance. The role of environment in the ecology C. burnetti, and its influence on seroconversion animals has not been elucidated Pakistan. We carried out a cross-sectional study Punjab province to (1) determine prevalence distribution soil using ISIIII gene-based real time-polymerase chain reaction (RT-PCR) assay, (2) analyze association between occurrence predictors i.e. characteristics (macro-...
Abstract Avian influenza A(H9N2) virus isolated from a poultry worker in Pakistan 2015 was closely related to viruses detected farms. Observed mutations the hemagglutinin receptor-binding affinity and antigenicity could affect cross-reactivity with prepandemic H9N2 vaccine strains.
Commercial poultry is an important agricultural industry worldwide. Although dense living conditions and large flocks increase meat egg production, they also the risk of disease outbreaks zoonoses. Current pathogen identification methods mostly rely on culture-dependent techniques and, therefore, are limited to a very small number bacteria present in environment. Next Generation Sequencing allows for culture-independent characterization lower respiratory microbiome birds including novel...
The co-occurrence of diabetes mellitus (DM) and tuberculosis (TB) is largely associated with high frequency morbidity.To determine the prevalence DM among TB patients describe socio-demographic behavioral factors TB-DM co-occurrence.We enrolled 500 from September, 2014 to August 2015 at four major public sector hospitals Lahore, Pakistan. A questionnaire was used collect information regarding patients. We monitored fasting blood sugar each patient by using a semi automated clinical chemistry...
The dairy industry is reshaping itself and becoming commercialized in Pakistan due to the increased demand for milk overcome shortage. Exotic breeds such as Holstein Friesian, a high producing breed have started being reared more on farms Pakistan. Along with other issues, mastitis does affects production of this breed. objective study was evaluate composition terms bacterial communities Friesian Punjab, alteration microbial healthy mastitic udder. Milk samples (n = 36) from rearing were...
The purpose of the present study was to obtain seroepidemiological information on Neospora caninum infection status sheep and goats in different areas Punjab Province Azad Kashmir (Pakistan). A cross-sectional study, with use a competitive ELISA, showed an overall 27.7% (35 128) (95% confidence interval [CI] ± 7.7%) 8.6% (13 142) CI 4.6%) seroprevalence N. antibodies goats, respectively. difference between goat populations statistically significant (P < 0.05). highest prevalence (37.4%...
The chicken industry of Pakistan is a major livestock sub-sector, playing pivotal role in economic growth and rural development. This study aimed to characterize map the structure broiler layer production systems, associated value chains, disease management Pakistan. Qualitative data were collected 23 key informant interviews one focus group discussion on types inputs, outputs, addition, market dynamics, management. Quantitative proportions commodity flows also obtained. Value chain maps...