A5091464568- Milk Quality and Mastitis in Dairy Cows
- Genetic and phenotypic traits in livestock
- Effects of Environmental Stressors on Livestock
- Mycobacterium research and diagnosis
- Reproductive Physiology in Livestock
- Cancer-related molecular mechanisms research
- Parasitic Infections and Diagnostics
- Viral Infections and Vectors
- Genetic Mapping and Diversity in Plants and Animals
- Animal Genetics and Reproduction
- Animal Diversity and Health Studies
- COVID-19 Clinical Research Studies
- Vector-Borne Animal Diseases
- Molecular Biology Techniques and Applications
- Probiotics and Fermented Foods
- Microbial infections and disease research
- Brucella: diagnosis, epidemiology, treatment
- Vector-borne infectious diseases
- Cytokine Signaling Pathways and Interactions
- Adipose Tissue and Metabolism
- SARS-CoV-2 and COVID-19 Research
- COVID-19 epidemiological studies
- Toxoplasma gondii Research Studies
- Antimicrobial Resistance in Staphylococcus
- COVID-19 Impact on Reproduction
University College London Hospitals NHS Foundation Trust
2025
University College London
2025
Abdul Wali Khan University Mardan
2015-2024
China Agricultural University
2013-2024
Baylor College of Medicine
2023
American College of Medical Genetics
2023
Shaikh Zayed Hospital
2022
Allama Iqbal Medical College
2021
CMH Lahore Medical College and Institute of Dentistry
2021
Government of Punjab
2020
Heat stress is known to affect follicular dynamics, oocyte maturation, and fertilization by impairing steroidogenic ability viability of bovine granulosa cell (bGCs). The present study explored the physiological molecular response bGCs different heat intensities in-vitro. We exposed primary (HS) at 39 °C, 40 °C 41 along with control samples (38 °C) for 2 h. To evaluate impact on bGCs, several in vitro cellular parameters including apoptosis, intracellular reactive oxygen species (ROS)...
We detected Crimean-Congo hemorrhagic fever virus infections in 4 provinces of Pakistan during 2017-2018. Overall, seroprevalence was 2.7% humans and 36.2% domestic livestock. Antibody prevalence highest rural areas, where increased contact with animals is likely.
Bovine mastitis is a widespread and costly disease that affects dairy farming globally, characterized by mammary gland inflammation. intramammary infection has been associated with more than 135 different pathogens of which Staphylococcus aureus the main etiology sub-clinical (SCM). The current study was designed to investigate prevalence, antibiotic resistance pattern, presence genes (mecA, tetK, aacA-aphD blaZ) in S. isolated from raw milk cows subclinical mastitis. A total 543 samples...
This research investigated farmers’ perception of the impact Presidential Fertilizer Initiative (PFI) programme (2016 - 2022) on crop production in Wukari, Nigeria. A multi-stage sampling procedure was employed to contact 160 farmers for study. well-structured questionnaire used data collection and using both descriptive inferential statistics such as mean score rating binary logit regression. The result indicates that about (56.9%) were males aged between 38 47 years (43.1%) with 0 10...
Abstract Introduction Clinical frailty scale (CFS) is used to generate a score ranging from 1 (very fit) 9 (terminally ill) for people aged ≥65 years. A CFS of ≥7 correlates with one-year mortality rate ~50%, making it useful identifying individuals potentially approaching last year life. NICE recommend this patient group are offered Advance care planning (ACP). ACP paramount ensuring receive high-quality, personalised end life care. We aimed investigate documentation and frequency...
Fasciola hepatica is a common parasite of grazing livestock. Fasciolosis burning problem for the livestock and dairy industry in Pakistan. The coproscopic examination was done fasciolosis associated risk factors were explored Nili Ravi Kundi buffaloes from various farms locality district Mardan. For this purpose, total 450 buffalo communities chosen random research faecal blood sampling. A subset (N=450) these divided into 2 groups based on species. Group (n = 225) Ravi, B Kundi. 5grams...
Theileriosis is an emerging issue of wild ungulates, especially in tropical and subtropical areas globally. The current study was designed to investigate Thelerial infection goats using microscopic identification its effect on haematological parameters. A total 300 blood samples were randomly collected from asymptomatic a convenient sampling technique. All the examined laboratory Clinical Medicine Parasitology simple microscopy. result showed that three hundred 34 % positive. highest...
Terrorism in Pakistan remains one of the most significant challenges, deeply impacting nation’s social fabric, political stability, and economic development. In 2024, regions Khyber Pakhtunkhwa (KPK) Islamabad have emerged as critical focal points for terrorist activities, posing severe threats to public safety governance. The country has witnessed a surge targeted attacks, including bomb blasts, armed assaults, suicide missions, primarily orchestrated by resurgent extremist groups. This...
Background: The SARS-COV-2's spread from continent to the has resulted in an increased number of mutations viral gene encoding proteins. As a result, target proteins provide significant challenge creating antiviral drugs and vaccines. present review discussed COVID-19 epidemiology effects being tested against COVID-19/SARS-COV-2. Dosage these along with associated challenges was also discussed. Methodology: Systematic conducted after thorough search "PubMed, NIH, Elsevier, Scopus, Web...
Isolation of Staphylococcus aureus (Staph. aureus) from Holstein milk samples with mastitis and nonmastitis was conducted to estimate its prevalence, antimicrobial resistance toxin genes. A total 353 were collected three Chinese herds. Fifty-three Staph. isolates 29 aureus-positive characterized via susceptibility, genes Pulsed-field Gel Electrophoresis (PFGE) profiles. The prevalence 4·0-9·5% in mastitic 7·3-11·5% nonmastitic the analysed Approximately 61·0% strains isolated cows resistant...
Perinatal period is the critical time in dairy cattle due to negative energy balance and high milk production stress. Being a key role biosynthesis methylation cycle, folic acid considered essential for lactational metabolic performance cattle. Thus, current study was designed evaluate effect of supplementation on phenotypic traits periparturient cows. Transcriptomic screening performed metabolism-associated differentially expressed genes. The 123 cows having similar parity, weight expected...
Staphylococcus aureus is a prominent cause of food-borne diseases worldwide. Enterotoxigenic strains this bacteria are frequently found in raw milk, and some these resistant to antimicrobials, posing risk consumers. The main objectives study were determine the antimicrobial resistance pattern S. milk detect presence mecA tetK genes it. A total 150 samples obtained aseptically from lactating cattle, including Holstein Friesian, Achai, Jersey breeds, maintained at different dairy farms....
Improvement for carcass traits related to beef quality is the key concern in production. Recent reports found that epigenetics mediates interaction of individuals with environment and nutrition. The present study was designed analyze genetic effect single nucleotide polymorphisms (SNPs) seven epigenetic-related genes (DNMT1, DNMT3a, DNMT3b, DNMT3L, Ago1, Ago2, HDAC5) two meat candidate (CAPN1 PRKAG3) on fourteen a Snow Dragon population, also identify SNPs total cattle populations. Sixteen...
Gastric cancer (GC) is one of the most common types gastrointestinal tumors worldwide, and side effects chemotherapeutic drugs resistance to chemotherapy remain problematic in its clinical treatment. Therefore, safe effective novel agents are urgently required. The purpose present study was investigate crocetin‑sensitive treatment GC possible mechanisms. BGC‑823 human cells were treated with crocetin. crocetin on viability determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium...
Isolation of sufficient quantities high quality DNA is a prerequisite for molecular studies.Milk somatic cells can be used; however, inhibitors such as fats and proteins make milk difficult medium extracting large amounts DNA.We optimized, evaluated compared three methods, Modified Nucleospin Blood Kit method, TianGen method Phenol-Chloroform genomic extraction from bovine milk.Individual cows' bulk samples were collected China agricultural university dairy farm.Genomic extracted each sample...
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans. Although dromedary camels are considered as major reservoir host, the MERS-CoV infection dynamics not fully understood. Through surveillance Pakistan, nasal (n = 776) and serum 1050) samples were collected from between November 2015 February 2018. Samples animal markets, free-roaming herds abattoirs. An in-house ELISA was developed to detect IgG against...
Bovine brucellosis remains a persistent infection in ruminants Pakistan. A total of 828 (409 buffaloes and 419 cattle) sera were collected from 11 institutional-owned livestock farms Punjab, The samples tested by rose bengal plate agglutination test (RBPT) indirect enzyme-linked immunosorbent assay (iELISA). seroprevalence along with 95% confidence interval (CI) was determined. Univariable multivariable analysis the epidemiological background data conducted odds ratio (OR) calculated to...
Aim: To determine the prevalence, attitude, and knowledge of self-medication general population for prevention coronavirus during COVID 19 pandemic. Place duration study: Major studies Punjab 2020. Methodology: A self-generated questionnaire was distributed online amongst excluding people using medication any other chronic medical disorder. The study included public including age group from 16-60 years. Results: Among 290 participants, 33.7% were males 66.3% female. Based on data obtained...
Background: Coronavirus disease 2019 (COVID-19) is a global health threat and caused universal psychosocial impact on the general population. Therefore, knowledge, attitude, perceptions (KAPs) of population are critical for development effective implementation standard operating procedures (SOP) to contain contagion minimize losses. current study was conducted understand evaluate KAPs Pakistani populations toward COVID-19. Methods: An online cross-sectional carried out among participants...
Guo, G., X., Wang, Y., Zhang, S., Li, Liu, L., Shi, W., Usman, T., Du, L. and Q. 2014. Estimation of genetic parameters fertility traits in Chinese Holstein cattle. Can. J. Anim. Sci. 94: 281–285. The objective this study was to estimate for heifers cows. Data 20169 animals with 42106 records over a period 10 yr (2001–2010) were collected from Sanyuan Lvhe Dairy Cattle Center Beijing, China. Traits included age at first service (AFS), number services (NS), days calving (CTFS), open (DO),...
Abstract The study described in this research communication used phylogenetic genotyping to identify virulence genes and antimicrobial susceptibility Escherichia coli recovered from cases of bovine mastitis. From 385 mastitic milk samples, 30 (7.8%) isolates were confirmed as E. coli. Most (80%) belonged phylo-group A. These also screened for 11 different genes. majority (63%) harbored no gene. Only (37%) tested positive two genes, either the iron uptake gene iucD 3 (10%) or serum resistance...
Abstract Background Staphylococcus aureus ( S. ) mastitis is one of the most difficult diseases to treat in lactating dairy cows worldwide. with different lineages leads host immune responses. Long non-coding RNAs (lncRNAs) are reported be widely involved progress inflammation. However, no research has identified stable lncRNAs among strain infections. In addition, folic acid (FA) can effectively reduce inflammation, and whether inflammatory response caused by reduced FA remains explored....
Abstract Improving the production traits and resistance against mastitis in dairy cattle is a challenge for animal scientists across globe. The present study was designed to investigate genetic effects of single nucleotide polymorphisms (SNPs) Janus kinase 2 ( JAK2 ) diacylglycerol acyltransferase DGAT1 genes with mastitis-related traits. Four SNPs one were analyzed through Chinese Cow's Chip-I (CCSC-I) genotyped population 312 Holsteins. Our findings demonstrated that milk fat percentage,...