A5040026799- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Hepatitis C virus research
- Liver Disease and Transplantation
- Cell death mechanisms and regulation
- Hepatitis B Virus Studies
- Alcohol Consumption and Health Effects
- Organ Transplantation Techniques and Outcomes
- RNA Interference and Gene Delivery
- Diet, Metabolism, and Disease
- Eicosanoids and Hypertension Pharmacology
- Leprosy Research and Treatment
- Drug-Induced Hepatotoxicity and Protection
- Cancer Research and Treatments
- Diabetes and associated disorders
- Chronic Lymphocytic Leukemia Research
- Pharmacological Effects and Toxicity Studies
- Cancer-related Molecular Pathways
- Pancreatitis Pathology and Treatment
- Connective Tissue Growth Factor Research
- interferon and immune responses
- Congenital Heart Disease Studies
- Liver Diseases and Immunity
- Tuberculosis Research and Epidemiology
- Immunodeficiency and Autoimmune Disorders
Kanazawa Medical University
2014-2024
Birmingham Children's Hospital
2024
Kanazawa Medical University Hospital
2020-2024
Mayo Clinic in Florida
2015-2024
Hospital for Sick Children
2024
Great Ormond Street Hospital
2022-2023
University College London
2022-2023
Seva Mandir
2022-2023
Emirates Aviation University
2023
University of Nebraska Medical Center
2021
Abstract A key feature in the pathogenesis of liver fibrosis is fibrillar Collagen-I deposition; yet, mediators that could be therapeutic targets remain elusive. We hypothesized osteopontin (OPN), an extracellular matrix (ECM) cytokine expressed hepatic stellate cells (HSCs), drive fibrogenesis by modulating HSC pro-fibrogenic phenotype and expression. Recombinant OPN (rOPN) up-regulated protein primary HSCs a transforming growth factor beta (TGFβ)–independent fashion, whereas it...
Tissue engineering using living cells is emerging as an alternative to tissue or organ transplantation. The adult mesenchymal stem can be differentiated into multilineage cells, such adipocytes, chondrocytes, osteoblasts when cultured with specific growth factors. In the present investigation, we have studied effect of honeycomb collagen scaffolds for adhesion, differentiation and proliferation bone marrow-derived osteoblasts. Mesenchymal were isolated from 6-week old albino rat femur...
Background & Aims Osteopontin (OPN) is a matricellular protein that upregulates during pathogenesis of hepatic fibrosis. The present study was aimed to evaluate whether serum OPN could be used as biomarker assess the degree fibrosis in patients with hepatitis C virus (HCV) infection. Methods Needle biopsy performed on HCV and scored zero (F0), mild (F1), moderate (F2), severe (F3) liver cirrhosis (F4) based Masson's trichrome α-smooth muscle actin (α-SMA) staining. Serum levels were measured...
Endocan is a vascular endothelium-derived factor regulated by angiogenic factors.The aim of this study was to determine whether serum endocan levels are prognostic for survival in patients with hepatocellular carcinoma (HCC).Serum were measured 64 HCC who naïve treatment, eight apparently healthy subjects, and 68 liver cirrhosis; the latter two groups served as controls.Prognostic factors examined using Cox proportional hazards model.The median 1.145 ng/mL (range, 0.93-1.68ng/mL) 1.93...
Osteopontin (OPN) is a matricellular cytokine and stress-induced profibrogenic molecule that promotes activation of stellate cells during the pathogenesis hepatic fibrosis. We studied protective effects epigallocatechin-3-gallate (EGCG) to suppress oxidative stress, inhibit OPN expression, prevent experimentally induced Liver injury was with intraperitoneal injections N-nitrosodimethylamine (NDMA) in dose 1 mg/100 g body weight on 3 consecutive days week for 28 days. A group rats received...
Abstract Tissue engineering requires a mechanically stable, biocompatible, and biodegradable scaffold that permits cell adherence proliferation, allows preservation of cell‐specific properties, suitable for surgical implantations. In this study, honeycomb collagen sheet was used three‐dimensional (3D) cultures human skin fibroblasts characterized as an effective dermal tissue engineering. About 1‐mm‐thick sheets, prepared from bovine atelocollagen, cross‐linked by UV‐irradiation, sterilized...
Connective tissue growth factor (CTGF) is involved in inflammation, pathogenesis and progression of liver fibrosis. Matrix metalloproteinase-13 (MMP-13) cleaves CTGF releases several fragments, which are more potent than the parent molecule to induce The current study was aimed elucidate significance MMP-13 their downstream effects injury Hepatic fibrosis induced using intraperitoneal injections N-nitrosodimethylamine (NDMA) doses 10 μg/g body weight on three consecutive days each week over...
The pathogenesis of nonalcoholic steatohepatitis (NASH) is a two-stage process in which steatosis the "first hit" and an unknown "second hit." We hypothesized that "a binge" could be to develop NASH from obesity-induced simple steatosis. Thirty-week-old male Otsuka Long-Evans Tokushima fatty (OLETF) rats were administered 10 mL 10% ethanol orally for 5, 3, 2, 1 d/wk 3 consecutive weeks. As control, (OLET) same amount alcohol. Various biochemical parameters obesity, monitored serum liver...
Abstract Purpose: The induction of apoptotic pathways in cancer cells offers a novel and potentially useful approach to improve patient responses conventional chemotherapy. Tissue factor pathway inhibitor-2 (TFPI-2) is protease inhibitor that abundant the extracellular matrix highly expressed noninvasive but absent or undetectable invasive human glioblastoma cells. Experimental Design: Using recombinant adeno-associated viral vector carrying TFPI-2 cDNA, we stably U-251 cells, cell line. Our...
Background Chronic ingestion of ethanol increases acetaldehyde and leads to the production acetaldehyde-derived advanced glycation end-products (AA-AGE). We evaluated toxicity AA-AGE on hepatocytes studied role in pathogenesis alcoholic liver disease (ALD). Methods Rat hepatocyte cultures were treated with N-ethyllysine (NEL) or cell viability was using MTT assay. Male Wistar rats fed liquid diet containing 5% for 8 weeks following normal another 12 weeks. A group animals sacrificed at 4th,...
Objectives Pulmonary arterial hypertension (PAH) is characterized by intimal lesions, right ventricular hypertrophy, and adventitial thickening of pulmonary arteries with progressive hypertension. This investigation was aimed to examine the effects transgenic expression human matrix metalloproteinase-9 (MMP-9) in pathogenesis PAH. Methods PAH induced using serial subcutaneous administration monocrotaline (MCT). Right pressure measured through jugular vein a 1.4F Millar Mikro-tip...