A5080915286- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Hepatitis C virus research
- Liver physiology and pathology
- Eicosanoids and Hypertension Pharmacology
- Liver Disease and Transplantation
- Metabolomics and Mass Spectrometry Studies
- Diet, Metabolism, and Disease
- Pancreatic and Hepatic Oncology Research
- Hepatitis B Virus Studies
- Helicobacter pylori-related gastroenterology studies
- Pancreatitis Pathology and Treatment
- Hepatocellular Carcinoma Treatment and Prognosis
- Organ Transplantation Techniques and Outcomes
- Pharmacogenetics and Drug Metabolism
- Neuroendocrine Tumor Research Advances
- Drug-Induced Hepatotoxicity and Protection
- Advanced Glycation End Products research
- Peroxisome Proliferator-Activated Receptors
- Proteoglycans and glycosaminoglycans research
- Sulfur Compounds in Biology
- Epigenetics and DNA Methylation
- Gastroesophageal reflux and treatments
- Metabolism and Genetic Disorders
- Cholangiocarcinoma and Gallbladder Cancer Studies
Kanazawa Medical University
2014-2025
Kanazawa Medical University Hospital
2020-2025
Nara Medical University
1997-2007
Karolinska Institutet
2001
Medical University of South Carolina
2001
George Washington University
2001
Anton Proksch Institut
2001
University of Vienna
2001
Thomas Jefferson University
2001
Osaka City University
2001
Perivenular hepatocytes are the first cells within liver lobule to display signs of toxicity following long-term alcohol use. In an attempt explain this phenomenon, we have examined hepatic intralobular distribution in rats and man P450IIE1, a P-450 isozyme that not only oxidizes ethanol but is also inducible by agent. Frozen sections microsomes were prepared from male Sprague-Dawley pair-fed liquid diets containing 36% total calories as either or carbohydrate (control) for 10 21 days....
Since nonalcoholic steatohepatitis (NASH) may progress to cirrhosis, it is important differentiate NASH from simple steatosis, especially in its early stages. However, a liver biopsy cannot be performed all patients with fatty disease (NAFLD). We herein investigated whether serum biochemical markers are useful for predicting early-stage NASH.Nineteen steatosis and 66 (stage 1-2 Brunt's criteria) were studied. The area under the receiver operating characteristic curve (AUC) was used...
Hepatic sinusoidal lining cells were isolated from mature male rats (12 months old) and cultured in order to clarify the characteristics of Ito (fat-storing cells). Desmin staining was used as a marker isolation cells, highly purified desmin-positive cell fraction obtained. Less than 10% positive for vitamin A auto-fluorescence. However, number A-positive increased with treatment, they extended over half by first week treatment. The both desmin vimentin stainings. On other hand, Kupffer...
Background & Aims Osteopontin (OPN) is a matricellular protein that upregulates during pathogenesis of hepatic fibrosis. The present study was aimed to evaluate whether serum OPN could be used as biomarker assess the degree fibrosis in patients with hepatitis C virus (HCV) infection. Methods Needle biopsy performed on HCV and scored zero (F0), mild (F1), moderate (F2), severe (F3) liver cirrhosis (F4) based Masson's trichrome α-smooth muscle actin (α-SMA) staining. Serum levels were measured...
Endocan is a vascular endothelium-derived factor regulated by angiogenic factors.The aim of this study was to determine whether serum endocan levels are prognostic for survival in patients with hepatocellular carcinoma (HCC).Serum were measured 64 HCC who naïve treatment, eight apparently healthy subjects, and 68 liver cirrhosis; the latter two groups served as controls.Prognostic factors examined using Cox proportional hazards model.The median 1.145 ng/mL (range, 0.93-1.68ng/mL) 1.93...
Osteopontin (OPN) is a matricellular cytokine and stress-induced profibrogenic molecule that promotes activation of stellate cells during the pathogenesis hepatic fibrosis. We studied protective effects epigallocatechin-3-gallate (EGCG) to suppress oxidative stress, inhibit OPN expression, prevent experimentally induced Liver injury was with intraperitoneal injections N-nitrosodimethylamine (NDMA) in dose 1 mg/100 g body weight on 3 consecutive days week for 28 days. A group rats received...
Connective tissue growth factor (CTGF) is involved in inflammation, pathogenesis and progression of liver fibrosis. Matrix metalloproteinase-13 (MMP-13) cleaves CTGF releases several fragments, which are more potent than the parent molecule to induce The current study was aimed elucidate significance MMP-13 their downstream effects injury Hepatic fibrosis induced using intraperitoneal injections N-nitrosodimethylamine (NDMA) doses 10 μg/g body weight on three consecutive days each week over...
The pathogenesis of nonalcoholic steatohepatitis (NASH) is a two-stage process in which steatosis the "first hit" and an unknown "second hit." We hypothesized that "a binge" could be to develop NASH from obesity-induced simple steatosis. Thirty-week-old male Otsuka Long-Evans Tokushima fatty (OLETF) rats were administered 10 mL 10% ethanol orally for 5, 3, 2, 1 d/wk 3 consecutive weeks. As control, (OLET) same amount alcohol. Various biochemical parameters obesity, monitored serum liver...
It has been reported that basement membranes were found around the sinusoidal walls in cirrhotic livers, indicating development of capillarization sinusoids. also emphasized sinusoids is more prominent alcoholic liver disease (ALD). In present study, factor VIII related antigen (VIII-Ag) and UEA-1 identified immunohistochemically order to analyze chronic diseases. Electron microscopic studies on endothelial cells performed. revealed number fenestra decreased clearly observed space Disse from...
Cytochrome P‐450 (CYP) 2E1 is the major ethanol‐oxidizing enzyme of nonalcohol dehydrogenase metabolic pathway in liver. Recently, presence genetic polymorphisms this was confirmed. In study, to clarify influence CYP2E1 genotype on alcohol metabolism, we analyzed acetaminophen metabolism subjects with different genotypes. normal subjects, a half‐life from blood longest type A ( c1/c1 ) and shortest C c2/c2 ). The elimination rate more than twice that B c1/c2 A, both were not between patients...
Progression of alcoholic liver disease is closely related to drinking habits. However, prognosis not determined just by habits, but also other factors. In this study, the roles alcohol-altered membrane antibody and hepatitis C virus infection were analyzed in patients who followed up for various lengths time. Serial changes histological appearance 39 with long periods (49.7 +/- 34.3 mo) underwent biopsy at least two times. Prognoses 35 cirrhosis more than 1 yr evaluated. Development was...
Background Chronic ingestion of ethanol increases acetaldehyde and leads to the production acetaldehyde-derived advanced glycation end-products (AA-AGE). We evaluated toxicity AA-AGE on hepatocytes studied role in pathogenesis alcoholic liver disease (ALD). Methods Rat hepatocyte cultures were treated with N-ethyllysine (NEL) or cell viability was using MTT assay. Male Wistar rats fed liquid diet containing 5% for 8 weeks following normal another 12 weeks. A group animals sacrificed at 4th,...