A5088751960- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Metabolism and Genetic Disorders
- DNA and Nucleic Acid Chemistry
- Innovations in Medical Education
- Neonatal Health and Biochemistry
- Genetics and Neurodevelopmental Disorders
- Endometrial and Cervical Cancer Treatments
- Diet and metabolism studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sarcoma Diagnosis and Treatment
- Reproductive Physiology in Livestock
- Anesthesia and Pain Management
- Global Maternal and Child Health
- Reproductive Health and Contraception
- Dendrimers and Hyperbranched Polymers
- Empathy and Medical Education
- Endometriosis Research and Treatment
- Liver Disease Diagnosis and Treatment
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Oral health in cancer treatment
- Folate and B Vitamins Research
- Glycogen Storage Diseases and Myoclonus
- Mosquito-borne diseases and control
University of Michigan
2016-2025
Michigan United
2022-2025
Khyber Medical University
2025
CMH Lahore Medical College and Institute of Dentistry
2020-2024
Khyber Medical College
2024
Vanderbilt University Medical Center
2022-2024
Khyber Teaching Hospital
2024
Jinnah Postgraduate Medical Center
2024
Combined Military Hospital
2020-2024
Memorial Hospital
2024
Gene therapy holds great promise as a future approach to fighting disease and is explored in worldwide clinical trials. Cationic liposome (CL)−DNA complexes are prevalent nonviral delivery vector, but their efficiency requires improvement the understanding of mechanism action incomplete. As part our effort investigate structure-transfection relationships self-assembled CL−DNA vectors, we have synthesized new, highly charged (16+) multivalent cationic lipid, MVLBG2, with dendritic headgroup....
Abstract Background Gene carriers based on lipids or polymers—rather than engineered viruses—constitute the latest technique for delivering genes into cells gene therapy. Cationic liposome–DNA (CL‐DNA) complexes have emerged as leading nonviral vectors in worldwide therapy clinical trials. To arrive at therapeutic dosages, however, their efficiency requires substantial further improvement. Methods Newly synthesized multivalent (MVLs) enable control of headgroup charge and size. Complexes...
Clonal hematopoiesis (CH) is an age-associated phenomenon that increases the risk of hematologic malignancy and cardiovascular disease. CH thought to enhance disease through inflammation in peripheral blood.1 Here, we profile blood gene expression 66 968 single cells from a cohort 17 patients with 7 controls. Using novel mitochondrial DNA barcoding approach, were able identify separately compare mutant Tet methylcytosine dioxygenase 2 (TET2) methyltransferase 3A (DNMT3A) nonmutant...
Lipid-mediated delivery of DNA into cells holds great promise both for gene therapy and basic research applications. This paper describes the efficient facile synthesis characterization a new multivalent cationic lipid with double-branched headgroup structure The synthetic scheme can be extended to give lipids different charge, spacer, or chain length. chemical physical properties self-assembled complexes liposomes (CLs) indications why possess superior transfection properties. bears five...
Background Managing postoperative pain after mandibular excision and reconstruction remains challenging, using conventional opioid medications has a risk of side effects. Objective This study aimed to evaluate the effectiveness safety transdermal buprenorphine patch in managing acute following resection real-world clinical setting. Methodology A prospective, observational was conducted, involving 108 patients who underwent or between January December 2023. Following surgery, participants...
Background: Vaginal infections are prevalent among women, often caused by disruptions in the vaginal microbiota, leading to symptoms like abnormal discharge, itching, and discomfort. Bacterial vaginosis (BV) is a common condition characterized shift from Lactobacillus dominance anaerobic bacteria overgrowth, affecting reproductive-age women significantly. Understanding microbial ecology antibiotic susceptibility of pathogens crucial for effective management. The study aims identify bacterial...
Background: Text neck syndrome (TNS), a chronic progressive condition caused by excessive use of handheld devices, is growing health concern affecting millions worldwide. Objective: The aim this study was to compare and analyze the effective treatment protocol between Proprioceptive Neuromuscular Facilitation (PNF) technique mulligan mobilization on TNS. Methods: with ethical approval number GCUF/ERC/23/2419 quasi-experimental design used purposive sampling. Data collected from patients ages...
ABSTRACT Glycogen storage disease type Ia (GSDIa) is a rare, life‐threatening, inherited carbohydrate metabolism disorder caused by glucose‐6‐phosphatase (G6Pase) deficiency, which essential for glycogenolysis and gluconeogenesis. GSDIa management includes strict medically prescribed diet that typically daily uncooked cornstarch doses, including overnight, to maintain euglycemia. DTX401 an investigational adeno‐associated virus serotype 8 vector expressing the human G6PC1 gene encodes...
DNA condensation in vivo relies on electrostatic complexation with small cations or large histones. We report a synchrotron x-ray study of the phase behavior complexed synthetic cationic dendrimers intermediate size and charge. encounter unexpected structural transitions between columnar mesophases in-plane square hexagonal symmetries, as well liquidlike disorder. The isoelectric point is locus instability. A simple model proposed based competing long-range interactions short-range entropic...
At present, there is an unprecedented level of interest in the properties and structures complexes consisting DNA mixed with oppositely charged cationic liposomes (CLs). The arises because mimic natural viruses as chemical carriers into cells worldwide human gene therapy clinical trials. However, since our understanding mechanisms action CL-DNA interacting remains poor, significant additional insights discoveries will be required before development efficient suitable for long-term...
Motivated by its important role in gene delivery, we have studied the effect of cholesterol and analogs on transfection efficiency (TE) lamellar cationic liposome−DNA (CL−DNA) complexes vitro. Addition to low-transfecting DOTAP/DOPC−DNA increases TE, with 15 mol % already yielding 10-fold improvement. Steroids lacking alkyl tail only modestly enhance while molecules retaining it strongly TE. All steroid-containing CL−DNA exhibit structure. The increase experimentally determined membrane...
Intellectual disability (ID) affects about 3% of the population and has a male gender bias. Of at least 700 genes currently linked to ID, more than 100 have been identified on X chromosome, including KIAA2022. KIAA2022 is located Xq13.3 expressed in developing brain. The protein product KIAA2022, X‐linked Disability Protein Related Neurite Extension (XPN), developmentally regulated involved neuronal migration cell adhesion. clinical manifestations loss‐of‐function mutations described...