A5002590489- Inflammatory Myopathies and Dermatomyositis
- Muscle Physiology and Disorders
- Peroxisome Proliferator-Activated Receptors
- Cancer-related Molecular Pathways
- Estrogen and related hormone effects
- Vitamin D Research Studies
- Fatty Acid Research and Health
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer Cells and Metastasis
- HER2/EGFR in Cancer Research
- Eicosanoids and Hypertension Pharmacology
- Adipose Tissue and Metabolism
- Vitamin C and Antioxidants Research
- Bone health and treatments
- Genomics and Chromatin Dynamics
- Muscle and Compartmental Disorders
- RNA Interference and Gene Delivery
- Advanced Breast Cancer Therapies
- Thyroid Disorders and Treatments
- Plant Gene Expression Analysis
- Hereditary Neurological Disorders
- Histone Deacetylase Inhibitors Research
- Retinoids in leukemia and cellular processes
- Natural product bioactivities and synthesis
- FOXO transcription factor regulation
Université de Strasbourg
2020-2025
Centre National de la Recherche Scientifique
2020-2025
Institut de génétique et de biologie moléculaire et cellulaire
2020-2025
Inserm
2020-2025
University of Calabria
2012-2019
Baylor College of Medicine
2013
Wageningen University & Research
2005
Abstract The omega‐3 long chain polyunsaturated fatty acids, docosahexaenoic acid (DHA), and eicosapentaenoic (EPA), elicit anti‐proliferative effects in cancer cell lines animal models. Dietary DHA EPA can be converted to their ethanolamide derivatives, docosahexaenoyl ethanolamine (DHEA), eicosapentaenoyl (EPEA), respectively; however, few studies are reported on anti‐cancer activities. Here, we demonstrated that DHEA EPEA were able reduce viability MCF‐7 breast cells whereas they did not...
Abstract Background Androgens are anabolic steroid hormones that exert their function by binding to the androgen receptor (AR). We have previously established AR deficiency in limb muscles impairs sarcomere myofibrillar organization and decreases muscle strength male mice. However, despite numerous studies performed men rodents, signalling pathways controlled androgens via skeletal remain poorly understood. Methods Male skm−/y ( n = 7–12) female skm−/− mice 9), which is selectively ablated...
Exosomes—small membrane vesicles secreted by both normal and malignant cells upon fusion of endosomal multivesicular bodies (MVBs) with the plasma membrane—play an important role in cell-to-cell communication. During last decade, several reports have highlighted involvement these nanovesicles many aspects breast cancer development progression, but extracellular signals governing their generation not been completely unraveled. Here, we investigated obesity hormone leptin, a well-known...
Prostate cancer (PCa) is a leading cause of cancer-related deaths. The slow evolution precancerous lesions to malignant tumors provides broad time frame for preventing PCa. To characterize prostatic intraepithelial neoplasia (PIN) progression, we conducted longitudinal studies on Pten(i)pe-/- mice that recapitulate prostate carcinogenesis in humans. We found early PINs are hypoxic and hypoxia-inducible factor 1 alpha (HIF1A) signaling activated luminal cells, thus enhancing progression....
The SNPWave marker system, based on SNPs between the reference accessions Colombia-0 and Landsberg erecta (Ler), was used to distinguish a set of 92 Arabidopsis from various parts world. In addition, we these markers genotype three new recombinant inbred line populations for Arabidopsis, having Ler as common parent that crossed with Antwerp-1, Kashmir-2, Kondara. benefit using multiple contain many similar fact all are linked physical map facilitates quantitative comparison maps....
Stromal Derived Factor-1α (SDF-1α) and its cognate receptor CXCR4 play a key role in mediating breast cancer cell invasion metastasis. Therefore, drugs able to inhibit activation may add critical tools reduce tumor progression, especially the most aggressive form of disease. Peroxisome Proliferator-Activated Receptor (PPAR) γ, member nuclear superfamily, has been found downregulate gene expression different cells, however molecular mechanism underlying this effect is not fully understood....
Inflammatory myopathies (IM) are a group of severe autoimmune diseases, sharing some similarities, whose cause is unknown and treatment empirical.While C-protein-induced myositis (CIM), the most currently used mouse model IM, has removed roadblocks to understand improve it only been partially characterised its generation limited by poor reproducibility. This study aimed at optimising characterisation CIM. In silico analysis was run identify top three specific immunogenic regions C-protein....
Abstract Skeletal muscle is a dynamic tissue the size of which can be remodeled through concerted actions various cues. Here, we investigated skeletal transcriptional program and identified key tissue-specific regulatory genetic elements. Our results show that Myod1 bound to numerous enhancers in collaboration with glucocorticoid receptor (GR) control gene expression. Remarkably, activation controlled by these factors occurs direct contacts promoter region target genes, via CpG-bound...
The combined treatment with nanomolar doses of the PPARγ ligand Rosiglitazone (BRL) and RXR 9-cis‑retinoic acid (9RA) induces a p53-dependent apoptosis in MCF7, SKBR3 T47D human breast cancer cells. Since MCF7 cells express wild-type p53 protein, while harbor endogenous mutant p53, we elucidated mechanism through which ligands triggered apoptotic processes independently transcriptional activity. We showed an upregulation Bid expression enhancing association between Bid/p53 both cytosol...
The active vitamin D metabolites, 25-hydroxyvitamin D3 (25D3) and 1,25-dihydroxyvitamin (1,25D3), are produced by successive hydroxylation steps play key roles in several cellular processes. However, alternative metabolic pathways exist, among them, the 4-hydroxylation of 25D3 is a major one. This study aims to investigate structure–activity relationships 4-hydroxy derivatives 1,25D3. Structural analysis indicates that 1,4α,25(OH)3D3 1,4β,25(OH)3D3 maintain anchoring hydrogen bonds 1,25D3...
The bioactive vitamin D3, 1α,25(OH)2D3, plays a central role in calcium homeostasis by controlling the activity of D receptor (VDR) various tissues. Hypercalcemia secondary to high circulating levels D3 leads hypercalciuria, nephrocalcinosis and renal dysfunctions. Current therapeutic strategies aim at limiting intake, absorption resorption, or 1α,25(OH)2D3 synthesis, but are poorly efficient. In this study, we identify WBP4 as new VDR interactant, demonstrate that it controls subcellular...
A large body of evidence suggests that dietary n-3 polyunsaturated fatty acids (PUFAs), including eicosapentaenoic acid (EPA) and docosahexaenoic (DHA), contribute to a reduced inflammatory tone thereby lowering the risk for several chronic degenerative diseases. Different mechanisms have been proposed explain these anti-inflammatory effects, those involving endocannabinoids endocannabinoid-like molecules. In this context, amides (FAAs), conjugates with amines or amino acids, are an emerging...
Vitamin D receptor (VDR) antagonists prevent the VDR activation function helix 12 from folding into its active conformation, thus affecting coactivator recruitment and antagonizing transcriptional regulation induced by 1α,25-dihydroxyvitamin D3. Here, we report crystal structure of zebrafish ligand-binding domain in complex with ZK168281 antagonist, revealing that ligand prevents optimal C-terminal region VDR. This interference was confirmed hydrogen-deuterium exchange mass spectrometry...
The Vitamin D receptor (VDR) plays a key role in calcium homeostasis, as well cell proliferation and differentiation. Among the large number of VDR ligands that have been developed, we previously shown BXL-62 Gemini-72, two C-20-modified vitamin analogs are highly potent agonists. In this study, show both restore transcriptional activities variants unresponsive to natural ligand identified patients with rickets. elucidated mechanisms action underlying these emphasize mutual adaptation...
Introduction Inflammatory myopathies (IM) are a group of severe autoimmune diseases, sharing some similarities, whose cause is unknown and treatment empirical. While C-protein-induced myositis (CIM), the most currently used model IM, has removed roadblock to understand improve it only been partially characterised its generation limited by reproducibility issues. This study aimed at optimising characterisation CIM. Methods In silico analysis was run identify top-3 specific immunogenic regions...
<h3>Background:</h3> Glucocorticoids (GC) treatment in myositis is empirical and side effects are frequent. Both therapeutic iatrogenic of GC mediated by glucocorticoid receptor (GR) which ubiquitously expressed. We have recently shown experimental (EM) induced GR(i)skm-/- mice that prednisone (PDN) induces a myofibre-mediated anti-inflammatory phenotype immune cells. Muscle transcriptomic analysis indicated PDN myofibres release epinephrine could mediate response. Epinephrine effect depends...
<h3>Background</h3> Glucocorticoids (GC) are the first line treatment in myositis. GC is empirical. Both therapeutic and iatrogenic effects mediated by glucocorticoid receptor (GR), which ubiquitously expressed. Our team has recently shown that muscle fibres immuno-metabolic modifications participate to weakness perpetuation of disease<sup>[1]</sup>. Thus, myofibers could be a target GC. <h3>Objectives</h3> To unravel mechanism effect order optimize myositis care. <h3>Methods</h3>...
<h3>Background</h3> Dermatomyositis (DM) is an autoimmune myopathy responsible for muscle weakness associated with decreased quality of life and increased mortality. Current treatments are empirical, partially effective, expose to a risk side effects high rate relapse upon discontinuation. DM muscular histology characterized by lesions perifascicular fibers consisting atrophy, overexpression type I interferon-dependent genes mitochondrial dysfunctions unknown origin [1]. <h3>Objectives</h3>...