A5102752763- Protein Structure and Dynamics
- Advanced biosensing and bioanalysis techniques
- Heat shock proteins research
- Microfluidic and Bio-sensing Technologies
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Characterization and Applications of Magnetic Nanoparticles
- Microbial Inactivation Methods
- Electrowetting and Microfluidic Technologies
- Biosensors and Analytical Detection
- Nerve injury and regeneration
- Advanced Biosensing Techniques and Applications
- Neurogenesis and neuroplasticity mechanisms
- Advanced Fluorescence Microscopy Techniques
- Fungal and yeast genetics research
- Spinal Cord Injury Research
University of Chicago
2024
University of Illinois Chicago
2024
Center for Neurosciences
2015
Abstract Multiplexed, real-time fluorescence detection at the single-molecule level can reveal stoichiometry, dynamics and interactions of multiple molecular species in mixtures other complex samples. However, fluorescence-based sensing is typically limited to just 3–4 colours a time due low signal-to-noise ratio, high spectral overlap need maintain chemical compatibility dyes. Here we engineered palette several dozen composite fluorescent labels, called FRETfluors, for multiplexed...
Axons in the adult CNS have poor ability to grow after injury, impeding functional recovery patients of spinal cord injury. This has been attributed both a developmental decline neuron-intrinsic growth and presence extrinsic inhibitors. We previously showed that genetic deletion Nogo, an inhibitor, promoted axonal sprouting from uninjured corticospinal tract (CST) neurons but not regeneration injured CST neurons, whereas PTEN, intrinsic regeneration. Here we test hypothesis combining...
Abstract Cells must sense and respond to sudden maladaptive environmental changes—stresses—to survive thrive. Across eukaryotes, stresses such as heat shock trigger conserved responses: growth arrest, a specific transcriptional response, biomolecular condensation of protein mRNA into structures known stress granules under severe stress. The composition, formation mechanism, adaptive significance, even evolutionary conservation these condensed remain enigmatic. Here we provide remarkable view...