A5082921919- Microfluidic and Bio-sensing Technologies
- Nanopore and Nanochannel Transport Studies
- Microfluidic and Capillary Electrophoresis Applications
- Advanced biosensing and bioanalysis techniques
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Advanced Fluorescence Microscopy Techniques
- Heat shock proteins research
- Photosynthetic Processes and Mechanisms
- Ion-surface interactions and analysis
- Electrostatics and Colloid Interactions
- Algal biology and biofuel production
- Fuel Cells and Related Materials
- Radiation Detection and Scintillator Technologies
- Electrohydrodynamics and Fluid Dynamics
- Spectroscopy and Quantum Chemical Studies
- Advanced Biosensing Techniques and Applications
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Medical Imaging Techniques and Applications
- Diffusion and Search Dynamics
- Membrane Separation Technologies
- DNA and Nucleic Acid Chemistry
- Mechanical and Optical Resonators
- Membrane-based Ion Separation Techniques
University of Chicago
2020-2024
Chan Zuckerberg Initiative (United States)
2024
Chan Zuckerberg Biohub Chicago
2024
University of Illinois Chicago
2024
Stanford University
2016-2022
Palo Alto University
2018
Stanford Medicine
2018
Boston University
2007-2017
Abstract Multiplexed, real-time fluorescence detection at the single-molecule level can reveal stoichiometry, dynamics and interactions of multiple molecular species in mixtures other complex samples. However, fluorescence-based sensing is typically limited to just 3–4 colours a time due low signal-to-noise ratio, high spectral overlap need maintain chemical compatibility dyes. Here we engineered palette several dozen composite fluorescent labels, called FRETfluors, for multiplexed...
Abstract Cells must sense and respond to sudden maladaptive environmental changes—stresses—to survive thrive. Across eukaryotes, stresses such as heat shock trigger conserved responses: growth arrest, a specific transcriptional response, biomolecular condensation of protein mRNA into structures known stress granules under severe stress. The composition, formation mechanism, adaptive significance, even evolutionary conservation these condensed remain enigmatic. Here we provide remarkable view...
Solid-state nanopores show promise as single-molecule sensors for biomedical applications, but to increase their resolution and efficiency, analyte molecules must remain longer in the nanopore sensing volume. Here we demonstrate a novel, facile, customizable sensor modification that reduces double-stranded DNA translocation velocity by 2 orders of magnitude or more via interactions outside nanopore. This is achieved electrospinning copolymer nanofiber mesh (NFM) directly onto solid-state...
Abstract Transcription factor (TF)-DNA interactions are the primary control point in regulation of gene expression. Characterization these is essential for understanding genetic biological systems and developing novel therapies to treat cellular malfunctions. Solid-state nanopores a highly versatile class single-molecule sensors that can provide rich information about local properties long charged biopolymers using current blockage patterns generated during analyte translocation platform...
Abstract The Orange Carotenoid Protein (OCP) is a cytosolic photosensor that responsible for non-photochemical quenching (NPQ) of the light-harvesting process in most cyanobacteria. Upon photoactivation by blue-green light, OCP binds to phycobilisome antenna complex, providing an excitonic trap thermally dissipate excess energy. At present, both binding site and NPQ mechanism are unknown. Using Anti-Brownian ELectrokinetic (ABEL) trap, we isolate single phycobilisomes free solution, presence...
Abstract The ability to quickly and reliably fabricate nanoscale pore arrays in ultra‐thin membranes such as silicon nitride (Si x N) is extremely important for the growing field of nanopore biosensing. Laser‐based etching thin Si N immersed aqueous solutions has recently been demonstrated a method produce stable functional pores. Herein, principal mechanism governing material formation using light investigated. It found that process sensitive relative content over atoms amorphous membrane,...
Nanopore sensing has enabled label-free single-molecule measurements on a wide variety of analytes, including DNA, RNA, and protein complexes. Much progress been made toward biotechnological applications; however, electrically probing the ion current introduces nonideal noise components. Here we further develop method to couple an ionic photon-by-photon counting fluorescent signal from Ca2+-sensitive dyes demonstrate optical detection biopolymer translocation through solid-state nanopores...
Optical sensing of solid-state nanopores is a relatively new approach that can enable high-throughput, multicolor readout from collection nanopores. It therefore highly attractive for applications such as nanopore-based DNA sequencing and genotyping using barcodes. However, to date optical has been plagued by the need achieve sufficiently high signal-to-noise ratio (SNR) single fluorophore sensing, while still maintaining millisecond resolution. One main factors degrading SNR in...
Frontal polymerization (FP) is an approach for thermosetting plastics at a lower energy cost than autoclave. The potential to generate simultaneous propagation of multiple fronts has been discussed as exciting possibility. However, FP initiated more two points simultaneously not demonstrated. Multipoint initiation could enable both large-scale material fabrication and unique pattern generation. Here, the authors present laser-patterned photothermal heating method locations in 2-D sample....
Significance The efficiency and adaptability of photosynthetic antenna systems in harvesting solar energy can be ultimately traced to their nanoscale organization. By monitoring the fluorescence emission from single proteins free solution over long timescales, we directly observed transitions among many distinct photophysical states exhibiting different flows transfer embedded photosensitive pigments. We show that these are well-described by a simple physical model, which allows us identify...
Anti-Brownian traps confine single particles in free solution by closed-loop feedback forces that directly counteract Brownian motion. Extended-duration measurements on trapped objects allow detailed characterization of photophysical and transport properties as well observation infrequent or rare dynamics. However, this approach has been generally limited to can be tracked fluorescence emission. Here we present the Interferometric Scattering ELectrokinetic (ISABEL) trap, which uses...
Abstract Solid‐state nanopores are an emerging biosensor for nucleic acid and protein characterization. For use in a clinical setting, solid‐state nanopore sensing requires sample preparation purification, fluid handling, heating element, electrical noise insulators, readout detector, all of which hamper its translation to point‐of‐care diagnostic device. A stand‐alone microfluidic‐based device is described that combines bioassay reaction/purification chamber with sensor. The microfluidic...
Most functional transcription factor (TF) binding sites deviate from their 'consensus' recognition motif, although and flanking sequences are often conserved across species. Here, we used single-molecule DNA unzipping with optical tweezers to study how Egr-1, a TF harboring three zinc fingers (ZF1, ZF2 ZF3), is modulated by the sequence context of its in Lhb gene promoter. We find that both core 9 bp bound Egr-1 each sites, base pairs them, modulate affinity structure protein-DNA complex....
In clinical settings, rapid and accurate characterization of pathogens is essential for effective treatment patients; however, subtle genetic changes in which elude traditional phenotypic typing may confer dangerous pathogenic properties such as toxicity, antibiotic resistance, or virulence. Existing options molecular techniques characterize the critical genomic that distinguish harmful benign strains, yet well-established approaches, particular those rely on electrophoretic separation...