A5016377004- Nanopore and Nanochannel Transport Studies
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Force Microscopy Techniques and Applications
- Supramolecular Self-Assembly in Materials
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Fuel Cells and Related Materials
- Quantum Dots Synthesis And Properties
- Silk-based biomaterials and applications
- Carbon Nanotubes in Composites
- Cellular Mechanics and Interactions
- Advanced Materials and Mechanics
- Graphene and Nanomaterials Applications
- Advanced Biosensing Techniques and Applications
- Anodic Oxide Films and Nanostructures
- Clostridium difficile and Clostridium perfringens research
- Membrane-based Ion Separation Techniques
- Surface Modification and Superhydrophobicity
- Advanced Polymer Synthesis and Characterization
- Molecular Junctions and Nanostructures
- Transition Metal Oxide Nanomaterials
- Polymer Surface Interaction Studies
Boston University
2016-2020
University of Maryland, College Park
2012-2013
Enteric Escherichia coli survives the highly acidic environment of stomach through multiple acid resistance (AR) mechanisms. The most effective system, AR2, decarboxylates externally-derived glutamate to remove cytoplasmic protons and excrete GABA. first described AR1, does not require an external amino acid. Its mechanism has been determined. regulation AR systems their coordination with broader cellular metabolism fully explored.We utilized a combination ChIP-Seq gene expression analysis...
Immobilization of biosensors in or on a functional material is critical for subsequent device development and translation to wearable technology. Here, we present the assessment an immobilized quantum dot–transcription factor–nucleic acid complex progesterone detection as first step toward such integration. The sensor, composed polyhistidine-tagged transcription factor linked dot fluorophore-modified cognate DNA, embedded within hydrogel immobilization matrix. optically transparent, soft,...
Monitoring individual proteins in solution while simultaneously obtaining tertiary and quaternary structural information is challenging. In this study, translocation of the vascular endothelial growth factor (VEGF) protein through a solid-state nanopore (ssNP) produces distinct ion-current blockade amplitude levels durations likely corresponding to monomer, dimer, higher oligomeric states. Upon changing from non-reducing reducing condition, blockage events monomeric state dominate,...
Abstract Solid‐state nanopores are an emerging biosensor for nucleic acid and protein characterization. For use in a clinical setting, solid‐state nanopore sensing requires sample preparation purification, fluid handling, heating element, electrical noise insulators, readout detector, all of which hamper its translation to point‐of‐care diagnostic device. A stand‐alone microfluidic‐based device is described that combines bioassay reaction/purification chamber with sensor. The microfluidic...
Vascular endothelial cells respond to blood flow-induced shear stress. However, the mechanisms through which transduce mechanical signals cellular responses remain poorly understood. In this report, using tensile-force assays, immunofluorescence and atomic force microscopy, we demonstrate that immunoglobulin proline-rich receptor-1 (IGPR-1) responds stimulation increases stiffness of cells. We observed IGPR-1 is activated by stress tensile flow stress–mediated activation modulates remodeling...
Self-assembly of amyloid nanofiber is associated with both functional biological and pathological processes such as those in neurodegenerative diseases. Despite intensive studies, the stochastic nature process has made it difficult to elucidate a molecular mechanism for key nucleation event. Here we investigated silk-elastin-like peptide (SELP) using time-lapse lateral force microscopy (LFM). By repeated scanning single line on SELP-coated mica surface, observed sudden stepwise height...
ABSTRACT A mild and facile strategy to coat natural rubber (NR) with a hydrophilic coating is described that uses light photoinitiator copolymers. Five high molecular weight copolymers, composed of (e.g., 2‐hydroxyethyl acrylate [HEA], poly(ethylene glycol) [PEG], N ‐vinylpyrrolidone [NVP], ‐isopropylacrylamide [NIDAM], acrylic acid [AA]) hydrophobic benzophenone (BP) units, are synthesized evaluated for NR on UV irradiation. When the HEA/BP, NVP/BP, AA/BP macromolecular photoinitiators...
We investigate the effects of frequency and density a nanomechanical stimulus on nucleation growth silk-elastin-like protein polymer (SELP) nanofibers. Repetitive tappings are crucial to create areas potential molecular level mechanism was proposed. Using this technique mechanically guided nanofiber patterns were successfully created.
Beyond the MTD and LD<sub>50</sub>, use of novel ultra-high drug loaded nanoparticles.
Abstract Immobilization of biosensors on surfaces is a key step toward development devices for real‐world applications. Here the preparation, characterization, and evaluation surface‐bound transcription factor–nucleic acid complex analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists gold surface modified with thiolated Cy5 fluorophore‐labeled DNA allosteric factor (TetR) linked quantum dot (QD). Upon addition...
Poly (3,4-(2-methylene)propylenedioxythiophene) (PMProDot) nanotubes were synthesized within the pores of polycarbonate and further modified with styrene vinylcarbazole by a one step electrochemical method through methylene functional group. The enhanced electrochromic properties composite investigated using FTIR, UV/Vis absorbance spectroscopy, AFM.