A5011280671- Nanopore and Nanochannel Transport Studies
- DNA Repair Mechanisms
- Microfluidic and Capillary Electrophoresis Applications
- Physics of Superconductivity and Magnetism
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Microtubule and mitosis dynamics
- Magnetic properties of thin films
- CRISPR and Genetic Engineering
- ZnO doping and properties
- Electronic and Structural Properties of Oxides
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
- Ion-surface interactions and analysis
- Machine Learning and ELM
- Particle accelerators and beam dynamics
- Carbon Nanotubes in Composites
- Crystallography and molecular interactions
- Ubiquitin and proteasome pathways
- Solar Thermal and Photovoltaic Systems
- Energy Harvesting in Wireless Networks
- Nanowire Synthesis and Applications
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- solar cell performance optimization
Technion – Israel Institute of Technology
2015-2025
University of Copenhagen
2013-2015
Novo Nordisk Foundation
2013-2014
Cancer Research UK
2005-2008
Ben-Gurion University of the Negev
2003
FZI Research Center for Information Technology
1994-1997
Max Planck Society
1992
Replicative DNA damage bypass, mediated by the ubiquitylation of sliding clamp protein PCNA, facilitates survival a cell in presence genotoxic agents, but it can also promote genomic instability damage-induced mutagenesis. We show here that PCNA budding yeast is activated independently replication-dependent S phase checkpoint similar conditions involving accumulation single-stranded at stalled replication intermediates. The ssDNA-binding A (RPA), an essential complex involved most...
Abstract PICH is a SNF2 family DNA translocase that binds to ultra-fine bridges (UFBs) in mitosis. Numerous roles for have been proposed from protein depletion experiments, but consensus has failed emerge. Here, we report deletion of avian cells causes chromosome structural abnormalities, and hypersensitivity an inhibitor Topoisomerase II (Topo II), ICRF-193. ICRF-193-treated −/− undergo sister chromatid non-disjunction anaphase, frequently abort cytokinesis. co-localizes with Topo IIα on...
RNA quantification methods are broadly used in life science research and clinical diagnostics. Currently, real-time reverse transcription polymerase chain reaction (RT-qPCR) is the most common analytical tool for quantification. However, cases of rare transcripts or inhibiting contaminants sample, an extensive amplification could bias copy number estimation, leading to errors false diagnosis. Single-molecule techniques may bypass but commonly rely on fluorescence detection probe...
Multicolor fluorescence substantially expands the sensing capabilities of nanopores by complementing or substituting resistive pulsing signals. However, to date single-fluorophore detection in multiple color channels has proven be challenging primarily due high photoluminescence (PL) emanating from silicon nitride (SiN x) membrane. We hypothesize that bandgap titanium oxide (TiO2) would eliminate PL background when used as a substrate for nanopore, and hence enable individual fluorophore...
The ability to routinely identify and quantify the complete proteome from single cells will greatly advance medicine basic biology research. To meet this challenge of single-cell proteomics, single-molecule technologies are being developed improved. Most approaches, date, rely on analysis polypeptides, resulting digested proteins, either in solution or immobilized a surface. Nanopore biosensing is an emerging technique that circumvents surface immobilization optimally suited for long...
Emerging single-molecule protein sensing techniques are ushering in a transformative era biomedical research. Nevertheless, challenges persist realizing ultra-fast full-length sensing, including loss of molecular integrity due to fragmentation, biases introduced by antibodies affinity, identification proteoforms, and low throughputs. Here, method for parallel separation tracking is introduced, yielding multi-dimensional properties used their identification. Proteins tagged chemo-selective...
Monitoring individual proteins in solution while simultaneously obtaining tertiary and quaternary structural information is challenging. In this study, translocation of the vascular endothelial growth factor (VEGF) protein through a solid-state nanopore (ssNP) produces distinct ion-current blockade amplitude levels durations likely corresponding to monomer, dimer, higher oligomeric states. Upon changing from non-reducing reducing condition, blockage events monomeric state dominate,...
Solid-state nanopore sensing of ultralong genomic DNA molecules has remained challenging, as the must be controllably delivered by its leading end for efficient entry into nanopore. Herein, we introduce a sensor device designed electro-optical detection and sorting (300+ kilobase pair) DNA. The fluidic device, fabricated in-silicon anodically bonded to glass, uses pressure-induced flow an embedded pillar array controllable stretching delivery. Extremely low concentrations (50 fM) sample...
Mitochondrial DNA (mtDNA) quantification is crucial in understanding mitochondrial dysfunction, which linked to a variety of diseases, including cancer and neurodegenerative disorders. Traditional methods often rely on amplification-based techniques, can introduce bias lack the precision needed for clinical diagnostics. Solid-state nanopores, an emerging biosensing platform, have advantage offering single-molecule label-free approaches by enabling direct counting molecules without...
AbstractReplicative DNA damage bypass promotes cell viability in the presence of genotoxic agents but at same time may lead to mutations, thereby contributing genomic instability. In eukaryotes, is mediated by damage-induced ubiquitylation replication clamp protein, proliferating nuclear antigen (PCNA). We have recently shown that protein A (RPA), a single-stranded (ss)DNA-binding essential for replication, repair and recombination, required PCNA budding yeast. Both yeast mammalian cells,...
Abstract Accurate identification of both abundant and rare proteins hinges on the development single-protein sensing methods. Given immense variation in protein expression levels a cell, separation by weight would improve classification strategies. Upstream facilitates sample binning into smaller groups while also preventing sensor overflow, as may be caused highly cell lysates or clinical samples. Here, we scale bulk analysis method for separation, sodium dodecyl sulfate–polyacrylamide gel...
Medium energy ion scattering combined with channeling was applied to study the crystalline quality of ultrathin YBaCuO films on (100) SrTiO3 and MgO substrates. Films thicknesses between 3 6 nm were deposited by inverted cylindrical magnetron sputtering. Under optimized growth conditions c-axis oriented obtained minimum yield values 2%–12% (depending thickness) for 23% MgO. On surface a disordered region thickness about 0.6 independent substrate, film thickness, storage time under ambient...
Medium energy ion scattering in combination with an electrostatic high-resolution detector system was applied to study the initial growth of YBaCuO thin films on (100) SrTiO3 and MgO substrates. Ultrathin thicknesses range 0.4 3.6 nm were deposited by inverted cylindrical magnetron sputtering. From absolute determination coverage as a function depth following features deduced: both substrates grow blocks one unit cell full homogeneous film is achieved at 3.6-nm thickness. In stage...