A5029675723- Cancer Research and Treatments
- Bacteriophages and microbial interactions
- Advanced NMR Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- NMR spectroscopy and applications
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Solid-state spectroscopy and crystallography
- Nanoplatforms for cancer theranostics
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Bacterial Genetics and Biotechnology
- Calcium Carbonate Crystallization and Inhibition
- Immunotherapy and Immune Responses
- Biotin and Related Studies
- Evolution and Paleontology Studies
- Electron Spin Resonance Studies
- Bone Tissue Engineering Materials
- CAR-T cell therapy research
- Advanced Biosensing Techniques and Applications
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Alzheimer's disease research and treatments
- Advanced MRI Techniques and Applications
- Amino Acid Enzymes and Metabolism
Institute of Biomedical Sciences, Academia Sinica
2018-2025
Academia Sinica
2023-2024
National Yang Ming Chiao Tung University
2023
University of California, San Francisco
2018-2021
Chongqing Three Gorges Central Hospital
2019
California Institute of Technology
2015-2018
National Taiwan University
2005-2014
Zhejiang University
2009
Institute of Chemistry, Academia Sinica
2008
University of Arizona
1997-2002
Abstract The tumor microenvironment (TME) presents differential selective pressure (DSP) that favors the growth of cancer cells, and monovalent therapy is often inadequate in reversing cell dominance TME. In this work, we introduce bacteria as a foreign species to TME explore combinatorial treatment strategies alter DSP for eradication. We show cancer-selective chemotherapeutic agents fasting can provide strong selection against presence bacteria. Moreover, an immunogenic drug (oxaliplatin),...
Significance Next-generation sequencing (NGS) has allowed the comprehensive study of genome and transcriptome. However, a similarly broad, highly multiplexed, inexpensive method for proteomics using NGS remains elusive. Here, we describe phage display-based preselected antibodies that are genetically encoded capable simultaneous profiling hundreds cell-surface targets on cells in culture or singly at low cost without need chemical conjugation to purified antibodies. We use identify proteins...
Tumor necrosis factor α (TNF-α) is upregulated in a chronic inflammatory environment, including tumors, and has been recognized as pro-tumor many cancers. Applying the traditional TNF-α antibodies that neutralize activity, however, only exerts modest anti-tumor efficacy clinical studies. Here, we develop an innovative approach to target distinct from neutralization mechanism. We employed phage display yeast select non-neutralizing can piggyback on co-internalize into cells through receptor...
Bacterial outer membrane vesicles (OMVs) have emerged as promising vehicles for anticancer drug delivery due to their inherent tumor tropism, immune-stimulatory properties, and potential functionalization with therapeutic proteins. Despite advantages, the high lipopolysaccharide (LPS) endotoxin content in OMVs raises significant safety regulatory challenges. In this work, we produce LPS-attenuated LPS-free systematically assess effects of LPS modification on OMVs' physicochemical...
Real-time speech interaction, serving as a fundamental interface for human-machine collaboration, holds immense potential. However, current open-source models face limitations such high costs in voice data collection, weakness dynamic control, and limited intelligence. To address these challenges, this paper introduces Step-Audio, the first production-ready solution. Key contributions include: 1) 130B-parameter unified speech-text multi-modal model that achieves understanding generation,...
Statherin is an active inhibitor of calcium phosphate precipitation in the oral cavity. For many studies interaction between statherin and hydroxyapatite (HAp), samples are prepared by a direct mixing or its fragment with well-crystalline HAp crystals. In this work, sample precipitated presence peptide derived from N-terminal 15 amino acids (SN-15). The situ crystallites nanosized, leading to significant increase amount adsorbed on surface. enhancement NMR sensitivity allows, for first time,...
Significance Computational protein design tools use a bottom-up approach that allows for the testing of hypotheses on relationships between amino acid sequence, structure and stability, biological function. Here, we exploited two computational methods, docking sequence optimization, to create favorable protein–protein interaction identical proteins, resulting in novel homodimer. A stepwise proved useful: scaffold stabilization followed by interface achieve homodimerization. Our results...
Direct delivery of therapeutic genes is a promising approach for treating cancers and other diseases. The current human viral vectors, however, suffer from several drawbacks, including poor cell-type specificity difficult large-scale production. M13 phage provides an alternative vehicle gene therapy with engineerable specificity, but the low transduction efficiency seriously limits its translational application. In this work, we discovered important factors cells phages that greatly...
Due to its highly immunogenic nature and the great engineerability, filamentous phage has shown promising antitumor activities in preclinical studies. Previous designs of mainly focused on tumor targeting using a cancer-specific moiety displayed minor capsid protein, pIII. In this work, we developed new therapeutic platform phage, which major protein pVIII was utilized for displaying an cytokine. We showcased that 16.1-kD cytokine GM-CSF could be efficiently presented M13 particle 8 + type...
Steric zippers, where the residues of two neighboring β-sheet layers are tightly interdigitated, have been proposed as fundamental structural units amyloid fibrils by Eisenberg and co-workers. The steric zipper formed polypeptides containing palindromic sequence AGAAAAGA has a distinctive feature that distance between interdigitated is comparable to interstrand individual β-sheet. This motif great interest in study prion disease because highly conserved proteins different species. In this...
Phase transformation between calcite and aragonite is an important issue in biomineralization. To shed more light on the mechanism of this process at molecular level, we employ solid-state 43Ca NMR to study phase from as regulated by magnesium ions, with enrichment a level 6%. Using gas diffusion approach, Mg-calcite formed initially system subsequently transforms reaction time proceeds. Our data support dissolution-recrystallization for transition. We find that parameters are very similar...
Phosphotyrosine (pY) is one of the most highly studied posttranslational modifications that responsible for tightly regulating many signaling pathways in eukaryotes. Pan-specific pY antibodies have emerged as powerful tools understanding role these modifications. Nevertheless, structures not been reported pan-specific antibodies, greatly impeding further development integrating this ubiquitous modification using structure-guided designs. Here, we present first crystal two widely utilized...
ABSTRACT The boundary between Hemphillian and Blancan North American Land Mammal Ages has been elusive equivocal. Our work identifies that at about 4.9 to 5.0 Ma, based on paleomagnetic radioisotopic dating of strata producing mammals in Meadow Spring Valleys Lincoln County, eastern Nevada, the Pine Nut Mountains western Nevada. Magnetostratigraphic study Panaca Formation County yielded a composite section with six magnetozones. Healdsburg Tephra was identified near top Valley, thereby...
Proximity labeling (PL) and chemical cross-linking (XL) mass spectrometry are two powerful methods to dissect protein–protein interactions (PPIs) in cells. Although PL typically captures neighboring proteins within a range of 10–20 nm single bait protein, XL defines direct contacts 1 systemic manner. Here, we develop new method, named PL/XL-MS, harness the advantages both XL. PL/XL-MS can enrich subcellular compartment by simultaneously identify PPIs multiple from data. We applied human...
Significance MYC is a powerful transcription factor and when overexpressed can drive cancers. We broadly characterized the MYC-driven surfaceome in B cells prostate using proteomics. overexpression induces large bidirectional changes through broad up-regulation of >80 metabolite transporters important for cell proliferation paradoxical two receptors involved turnover such as TNF receptor family member natural killer activating ligand. targeted these recombinant bispecific T engagers...
Samples of carboxylate-fluorapatite are prepared with citric, tricarballylic, and glutaric acids under hydrothermal conditions. The size the hexagonal rods differs significantly for three samples, which citric-acid sample exhibits smallest dimension along [h00] direction. solid-state NMR data reveal that all citrate molecules citrate-fluorapatite in direct contact fluorapatite surface there at least two binding modes accounting interaction between surface. In addition to electrostatic...
Certain bacteria demonstrate the ability to target and colonize tumor microenvironment, a characteristic that positions them as innovative carriers for delivering various therapeutic agents in cancer therapy. Nevertheless, our understanding of how adapt their physiological condition microenvironment remains elusive. In this work, we employed liquid chromatography-tandem mass spectrometry examine proteome E. coli colonized murine tumors. Compared cultivated rich medium, found tumors notably...
Abstract The development of immune cell engagers (ICEs) can be limited by logistical and functional restrictions associated with fusion protein designs, thus limiting recruitment to solid tumors. Herein, a high affinity superantigen‐based multivalent ICE is developed for simultaneous activation NK T cells tumor treatment. Yeast library‐based directed evolution adopted identify superantigen variants possessing enhanced binding immunoreceptors expressed on human cells. High‐affinity...