A5034753947- Gut microbiota and health
- Probiotics and Fermented Foods
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- COVID-19 Prevention and Impact
- Pediatric Hepatobiliary Diseases and Treatments
- Drug Transport and Resistance Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Food composition and properties
- Enzyme Production and Characterization
- Nutritional Studies and Diet
- interferon and immune responses
- Helicobacter pylori-related gastroenterology studies
- Microbial Metabolites in Food Biotechnology
- Gastrointestinal motility and disorders
- Digestive system and related health
- Genomics and Phylogenetic Studies
- COVID-19 Clinical Research Studies
- Escherichia coli research studies
Keio University
2023-2025
Keio University Shonan Fujisawa
2024
Hokkaido University
2021
The coronavirus disease 2019 (COVID-19) pandemic has caused health issues worldwide. Studies have suggested that modulation of the gut microbiota could attenuate severity COVID-19 symptoms. In light this, we explored effects prebiotic dietary fibre partially hydrolyzed guar gum (PHGG) on SARS-CoV-2 infection in a Syrian hamster model, hypothesizing microbiome and intestinal metabolites through PHGG administration would improve outcomes. Eight hamsters each were assigned to control groups....
Abstract Current treatment options for COVID-19 are limited, with many antivirals and immunomodulators restricted to the most severe cases preventative care limited vaccination. As SARS-CoV-2 virus its increasing variants threaten become a permanent fixture of our lives, this new reality necessitates development cost-effective accessible COVID-19. Studies have shown that there correlations between gut microbiome severity COVID-19, especially regards production physiologically beneficial...
The human gut houses bile acid 7α-dehydroxylating bacteria that produce secondary acids such as deoxycholic (DCA) from host-derived through enzymes encoded by the bai operon. While recent metagenomic studies suggest these are highly diverse and abundant, very few DCA producers have been identified. Here, we investigated physiology determined complete genome sequence of Eubacterium sp. c-25, a producer was isolated feces in 1980s. Culture experiments showed preference for neutral to slightly...
The gut microbiota is closely associated with human health and greatly impacted by the host diet. Bacteria such as Escherichia coli live in all throughout life of a adapt to intestinal environment. Adaptive mutations E. are reported enhance fitness mammalian intestine, but what extent still poorly known. It also unknown whether diet affects genes mutated affected. This study suggests that genetic K-12 strain selected response environment facilitate efficient utilization abundant nutrients...
Since most genetically modified mice are C57BL/6 background, a mouse-adapted SARS-CoV-2 that causes lethal infection in young is useful for studying innate immune protection against infection. Here, we established two SARS-CoV-2, ancestral and Delta variants, by serial passaging 80 times mice. Although were resistant to with the variant caused In contrast, MyD88 IFNAR1 KO exhibited resistance variant. Treatment recombinant IFN-α/β at time of protected from variant, but intranasal...
Obligately anaerobic, Gram-stain-positive, bacilli, strains 12BBH14 T , 9CFEGH4 and 10CPCBH12, were isolated from faecal samples of healthy Japanese people. Strain showed the highest 16S rRNA gene sequence similarity to Sellimonas monacensis Cla-CZ-80 (97.5 %) ‘ Lachnoclostridium phocaeense ’ Marseille-P3177 (97.2 %). was also closely related Eubacterium sp. c-25 with 99.7 % similarity. The analysis that 10CPCBH12 formed a monophyletic cluster c-25. Near this cluster, S. L. phocaeense’ did...
<title>Abstract</title> Intestinal metabolites produced by gut microbes play a significant role in host health. Due to their different chemical structures, they are often analyzed using multiple analyzers and methods, such as gas chromatography/mass spectrometry (GC/MS) for SCFAs liquid (LC/MS) bile acids (BAs), amino (AAs), sugars. In this study, we aimed develop specialized method the simultaneous determination of important intestinal metabolites, specifically addressing main issue SCFA...
Intestinal metabolites produced by gut microbes play a significant role in host health. Due to their different chemical structures, they are often analyzed using multiple analyzers and methods, such as gas chromatography/mass spectrometry (GC/MS) for SCFAs liquid (LC/MS) bile acids (BAs), amino (AAs), sugars. In this study, we aimed develop specialized method the simultaneous determination of important intestinal metabolites, specifically addressing main issue SCFA volatilization during dry...
Irritable bowel syndrome (IBS) has no clinically accepted biomarkers even though it affects a large number of individuals worldwide. To address this lack understanding, we evaluated peptidase activity in fecal samples from 35 patients with diarrheal IBS without symptom exacerbation (IBS-n) and healthy subjects using library 384 fluorescent enzymatic substrate probes. IBS-n had high trypsin-like for cleavage C-terminal lysine arginine residues low elastase-like serine glycine residues. These...
Abstract Current treatment options for COVID-19 are limited, with many antivirals and immunomodulators restricted to the most severe cases preventative care limited vaccination. As SARS-CoV-2 virus its increasing variants threaten become a permanent fixture of our lives, this new reality necessitates development cost-effective accessible COVID-19. Studies have shown that there correlations between gut microbiome severity COVID-19, especially regards production physiologically beneficial...
Abstract The coronavirus disease 2019 (COVID-19) pandemic has caused worldwide health issues. Although several vaccines have been developed, it is still difficult to prevent and reduce the inflammation by infection. Studies shown that there are correlations between gut environment severity of symptoms SARS-CoV-2 Several metabolites produced microbiota such as SCFAs secondary bile acid UDCA reported improve survival rate host after viral infection in an animal model through modulation immune...