A5040760945- Plant nutrient uptake and metabolism
- Metabolomics and Mass Spectrometry Studies
- Plant responses to water stress
- Advanced Chemical Sensor Technologies
- Seaweed-derived Bioactive Compounds
- GABA and Rice Research
- Photosynthetic Processes and Mechanisms
- Echinoderm biology and ecology
- Plant Stress Responses and Tolerance
- Meat and Animal Product Quality
- Isotope Analysis in Ecology
- Biochemical Analysis and Sensing Techniques
- Plant Molecular Biology Research
- Rice Cultivation and Yield Improvement
- Sugarcane Cultivation and Processing
- Mass Spectrometry Techniques and Applications
- Polyamine Metabolism and Applications
- Marine and coastal plant biology
- Pharmacological Effects and Assays
- Phytoestrogen effects and research
- Escherichia coli research studies
- Plant Parasitism and Resistance
- Potato Plant Research
- Analytical Chemistry and Chromatography
- NMR spectroscopy and applications
Ehime University
2024
Keio University
2014-2024
Keio University Shonan Fujisawa
2020-2024
Tokyo University of Agriculture
2020
The University of Tokyo
2010-2012
Saitama University
2003-2006
National Agriculture and Food Research Organization
2006
Institute of Agrobiological Sciences
2003-2004
Summary The excessive application of nitrogen fertilizer to maximize crop yields causes negative environmental effects such as pollution and ecological imbalance. To overcome this problem, researchers have attempted improve the assimilation capacity crops. Maize Dof1 (ZmDof1) is a plant‐specific transcription factor shown promote in Arabidopsis thaliana (Arabidopsis) even under nitrogen‐deficient conditions. present study examines effect introduction ZmDof1 gene on carbon rice. induced...
• Background and Aims It is well documented that C4 grasses have a shorter distance between longitudinal veins in the leaves than C3 grasses. In grass leaves, however, with different structures functions are differentiated: large veins, small transverse veins. Thus, densities of three types vein were investigated from two-dimensional perspective.
A simple, low-cost capillary electrophoresis-mass spectrometry (CE-MS) method is demonstrated for the simultaneous analysis of amino acids and small carboxylic (glycerate, lactate, fumarate, succinate, malate, tartrate, citrate, iso-citrate, cis-aconitate, shikimate). All CE-MS experiments were performed using a single uncoated fused-silica with separation electrolyte, formic acid. For CE polarity, inlet was set as anode, MS side cathode. By high-speed sheath gas flow, apparent mobilities...
Glycerol, a byproduct of biodiesel, has become readily available and inexpensive carbon source for the production high-value products. However, main drawback glycerol utilization is low consumption rate shortage NADPH formation, which may limit NADPH-requiring To overcome these problems, we constructed catabolite repression-negative ΔptsGglpK* mutant by both blocking key glucose PTS transporter enhancing conversion. The can recover normal growth co-utilization after loss transporter. reveal...
Dry-cured hams are popular among consumers. To increase the attractiveness of product, objective analytical methods and algorithms to evaluate relationship between observable properties consumer acceptability required. In this study, metabolomics, which is used for quantitative profiling hundreds small molecules, was applied 12 kinds dry-cured from Japan Europe. total, 203 charged metabolites, including amino acids, organic nucleotides, peptides, were successfully identified quantified....
The excessive amounts of nitrogen applied in current farming systems can cause environmental problems. There is therefore a need to improve the ability crop plants utilize nitrogenous fertilizers. We screened for deficiency-tolerant lines among transgenic rice that overexpressed full-length cDNAs (FL-cDNAs) corresponding low-nitrogen response genes, genes related metabolism, and carbon metabolism. found overexpression OsCPK12 FL-cDNA, encoding calcium-dependent protein kinase (CDPK),...
Glutamate dehydrogenase (GDH) catalyzes the reversible amination of 2-oxoglutarate with ammonium to form glutamate. GDH functions in nitrogen assimilation microorganisms, such as Aspergillus nidulans. However, plants, glutamine synthetase, not GDH, carries out assimilation. Here, we report effects introduction gdhA gene, encoding NADP(H)-dependent glutamate dehydrogenase, from A. nidulans into potato. We analyzed resulting changes photosynthesis, biomass, carbon and contents under control...
The gut microbiota is closely associated with human health and greatly impacted by the host diet. Bacteria such as Escherichia coli live in all throughout life of a adapt to intestinal environment. Adaptive mutations E. are reported enhance fitness mammalian intestine, but what extent still poorly known. It also unknown whether diet affects genes mutated affected. This study suggests that genetic K-12 strain selected response environment facilitate efficient utilization abundant nutrients...
Quantitative nuclear magnetic resonance (qNMR) is an accepted method for determining analyte concentrations using quantitative substances in one spectrum. Conventional qNMR performed a mixture of analytes and reference substances. In coaxial-tube NMR, two tubes are used as different solutions, similar to normal NMR spectra. Currently, coaxial with various diameters available; however, limited, general analytical protocol yet be proposed. this study, we established effective volume ratio...
The leaf of the NADP-malic enzyme type C(4) grass, Arundinella hirta, has not only mesophyll cells (MCs) and bundle sheath (BSCs, usual Kranz cells) but also another (distinctive cells; DCs) that are associated with vascular bundles. We investigated photosynthetic accumulation along base-to-tip maturation gradient developing leaves by immunogold electron microscopy. In mature leaves, phosphoenolpyruvate carboxylase (PEPC) ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) were...
Abstract Flower opening time (FOT) is affected by genetic and environmental factors, but little known about the effect of light dark conditions on FOT in cereal crops. an indica rice cultivar, IR64, its near-isogenic line carrying a QTL for early-morning flowering trait (IR64+qEMF3) were investigated natural-light temperature-controlled small greenhouse exposing either panicle or stem both plant organs to different conditions. did not change genotype when panicles exposed light. A large...
A bacterial insertion sequence (IS) is a mobile DNA carrying only the transposase gene (tnp) that acts as mutator to disrupt genes, alter expressions, and cause genomic rearrangements. “Canonical” ISs have historically been characterized by their terminal inverted repeats (IRs), which may form stem-loop structure, duplications of short (non-IR) target at both ends, called site (TSDs). The IS distributions virulence potentials Staphylococcus aureus genomes in familial infection cases are...
Soil alkalinity due to the accumulation of alkaline salts greatly impairs crop production. Despite advancements in stress response studies through metabolomic analyses, limited progress has been made understanding cultivar differences alkali tolerance scarcity suitable genetic material. This study aimed characterize metabolic responses two rice cultivars, Kasalath (alkali-sensitive) and Gharib (alkali-tolerant), which were screened an soil field. A analysis hydroponically grown seedlings...