A5027012428- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Plant Micronutrient Interactions and Effects
- Plant responses to water stress
- Seed Germination and Physiology
- Rice Cultivation and Yield Improvement
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Phytase and its Applications
- Aluminum toxicity and tolerance in plants and animals
- Biofuel production and bioconversion
- Trace Elements in Health
- Enzyme Production and Characterization
- Plant Genetic and Mutation Studies
- Genetics, Bioinformatics, and Biomedical Research
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Plant Disease Resistance and Genetics
- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
The University of Tokyo
2015-2024
Hokkaido University
2016
Northeast Forestry University
2012-2016
Gunma University Hospital
2008
Hitachi (Japan)
2001
Yamagata University Hospital
2001
Tokyo University of Science
1997
JCR Pharmaceuticals (Japan)
1994
National Institutes of Health
1988-1989
National Institute of Environmental Health Sciences
1988
Pearl millet is a cereal crop known for its high tolerance to drought, heat and salinity stresses as well nutritional quality. The molecular mechanism of drought in pearl unknown. Here we attempted unravel the basis two inbred lines, ICMB 843 863 using RNA sequencing. Under greenhouse condition, was found be more tolerant than 863. We sequenced root transcriptome from both lines under control conditions an Illumina Hi-Seq platform, generating 139.1 million reads. Mapping reads against...
Abstract Abscisic acid is a stress-related phytohormone that has roles in dehydration and rehydration. In Arabidopsis (Arabidopsis thaliana), two genes inactivate abscisic acid, CYP707A1 CYP707A3, are rapidly up-regulated upon The factors regulate CYP707A1/3 not well characterized. We expressed bZIP protein, VIP1, as green fluorescent protein fusion found the nuclear localization of VIP1 was enhanced within 10 min after A yeast one-hybrid assay revealed amino-terminal region transcriptional...
A high-affinity K+transporter PutHKT2;1 cDNA was isolated from the salt-tolerant plant Puccinellia tenuiflora. Expression of induced by both 300 mM NaCl and K+-starvation stress in roots, but only slightly regulated those stresses shoots. transcript levels were doubled depletion potassium. Yeast transformed with PutHKT2;1, like PhaHKT2;1 reed plants (Phragmites australis), (i) able to take up K+ low concentration medium or presence NaCl, (ii) permeable Na+. This suggests that has a high...
The improvement and development of saline–alkali land is great significance for promoting food production sustainable development. It necessary to study the mechanism saline–alkaline tolerance breed tolerant crops improve utilization land. For this study, we conducted a three-year pot experiment screen germplasm 72 rice genotypes from hundreds elite cultivars during whole growth period using certain proportion soda soil. selected salt-tolerant variety was combined with salt-sensitive analyze...
Pearl millet (Pennisetum glaucum (L) R. Br.) is a staple crop for more than 90 million poor farmers. It known its tolerance against drought, salinity, and high temperature. To understand the molecular mechanisms underlying salinity tolerance, physiological analyses comparative transcriptome analysis between tolerant (ICMB 01222) susceptible 081) lines were conducted under control conditions. The studies revealed that line ICMB 01222 had higher growth rate accumulated amount of sugar in...
Pearl millet (Pennisetum glaucum) is a cereal crop that possesses the ability to withstand drought, salinity and high temperature stresses. The NAC [NAM (No Apical Meristem), ATAF1 (Arabidopsis thaliana Activation Factor 1), CUC2 (Cup-shaped Cotyledon)] transcription factor family one of largest families in plants. members are known regulate plant growth abiotic stress response. Currently, no reports available on functions pearl millet.Our genome-wide analysis found 151 genes (PgNACs)...
VIP1 is a bZIP protein in Arabidopsis thaliana. accumulates the nucleus under hypo-osmotic conditions and interacts with promoters of hypo-osmolarity-responsive genes, CYP707A1 CYP707A3 (CYP707A1/3), but neither overexpression nor truncation its DNA-binding region affects expression vivo, raising possibility that VIP other proteins are functionally redundant. Here we show further analyses on close homologs, namely, group I proteins. The patterns signals GFP-fused were similar onion cells,...
Puccinellia tenuiflora is the most saline-alkali tolerant plant in Songnen Plain, one of three largest soda lands worldwide. Here, we investigated physicochemical properties soils from Plain and sequenced transcriptomes germinated P. seedlings under long-term treatment (from seed soaking) with soil extracts.
Large areas of northern China have alkaline soil due to the accumulation sodium carbonates (NaHCO3, Na2CO3). To understand better how plants can tolerate soil, a cDNA library was prepared from rice (Oryza sativa L.) roots grown in presence NaHCO3 stress. A clone isolated this identified by homology search as mitochondrial ATP synthase 6 kDa subunit gene (RMtATP6; GenBank accession nos AB055076, BAB21526). In transformed yeast and tobacco protoplasts, RMtATP6 protein localized mitochondria...
Protein extraction is a frequent procedure in biological research. For preparation of plant cell extracts, materials usually have to be ground and homogenized physically break the robust wall, but this step laborious time-consuming when large number samples are handled at once.We developed chemical method for lysing Arabidopsis cells without grinding. In method, plants boiled just 10 minutes solution containing Ca2+ chelator detergent. Cell extracts prepared by were suitable SDS-PAGE...
Summary In plant cells, the vacuolar‐type H + ‐ ATP ases (V‐ ase) are localized in tonoplast, Golgi, trans ‐Golgi network and endosome. However, little is known about how V‐ ase influences growth, particularly with regard to c subunit ( VHA ‐c). Here, we characterized function of a ‐c gene from Puccinellia tenuiflora (Put ‐c) growth. Compared wild‐type, transgenic plants overexpressing Put Arabidopsis thaliana exhibit better growth phenotypes root length, fresh weight, height silique number...
VIP1 is a bZIP transcription factor in Arabidopsis (Arabidopsis thaliana). transiently accumulates the nucleus when cells are exposed to hypoosmotic conditions, but its physiological relevance unclear. This possibly because has approximately 10 close homologs of and they function redundantly. To examine their roles, transgenic plants overexpressing repression domain-fused form (VIP1-SRDXox plants), which gene activation mediated by expected be repressed, were generated. Because stress can...
Since NH4+ is one of the most important limiting nitrogen sources for plant growth, ammonium uptake and transport system has particular attention. In cells, transporters (AMTs) are responsible transport. previous studies, we identified a PutAMT1;1 gene from Puccinellia tenuiflora, which monocotyledonous halophyte species that thrives in alkaline soil. The overexpression Arabidopsis thaliana enhanced growth increased susceptibility to toxic methylammonium (MeA). This transporter might be...