A5038846192- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Metabolomics and Mass Spectrometry Studies
- Bacterial Genetics and Biotechnology
- Gene Regulatory Network Analysis
- CRISPR and Genetic Engineering
- Microbial Natural Products and Biosynthesis
- GABA and Rice Research
- Plant-Microbe Interactions and Immunity
- Microbial Fuel Cells and Bioremediation
- Vibrio bacteria research studies
- Bioinformatics and Genomic Networks
- Lichen and fungal ecology
- Fungal and yeast genetics research
- Plant Gene Expression Analysis
- Chemical Reactions and Isotopes
- Biochemical and biochemical processes
- Slime Mold and Myxomycetes Research
- Bacterial biofilms and quorum sensing
- Plant biochemistry and biosynthesis
- Transgenic Plants and Applications
Shanghai Jiao Tong University
2015-2024
Kyushu Institute of Technology
2011-2013
13C metabolic flux analysis (13C-MFA) has been widely used to measure in vivo enzyme reaction rates (i.e., flux) microorganisms. Mining the relationship between environmental and genetic factors fluxes hidden existing fluxomic data will lead predictive models that can significantly accelerate quantification. In this paper, we present a web-based platform MFlux (http://mflux.org) predicts bacterial central metabolism via machine learning, leveraging from approximately 100 13C-MFA papers on...
Abstract Background Most bacteria can use various compounds as carbon sources. These sources be either co-metabolized or sequentially metabolized, where the latter phenomenon typically occurs catabolite repression. From practical application point of view utilizing lignocellulose for production biofuels etc., it is strongly desirable to ferment all sugars obtained by hydrolysis from lignocellulosic materials, simultaneous consumption would benefit formation bioproducts. However, most...
Glycerol, an inevitable byproduct of biodiesel, has become attractive feedstock for the production value-added chemicals due to its availability and low price. Pseudomonas chlororaphis HT66 can use glycerol synthesize phenazine-1-carboxamide (PCN), a phenazine derivative, which is strongly antagonistic fungal phytopathogens. A systematic understanding underlying mechanisms PCN overproduction will be important further improvement industrialization.We constructed PCN-overproducing strain...
Glycerol, a byproduct of biodiesel, has become readily available and inexpensive carbon source for the production high-value products. However, main drawback glycerol utilization is low consumption rate shortage NADPH formation, which may limit NADPH-requiring To overcome these problems, we constructed catabolite repression-negative ΔptsGglpK* mutant by both blocking key glucose PTS transporter enhancing conversion. The can recover normal growth co-utilization after loss transporter. reveal...
Bioprocessing offers a sustainable and green approach to manufacture various chemicals materials. Development of bioprocesses requires transforming common producer strains cell factories. 13C metabolic flux analysis (13C-MFA) can be applied identify relevant targets accomplish the desired phenotype, which has become one major tools support systems engineering. In this research, we 13C-MFA bottlenecks in bioconversion glycerol into acetol by Escherichia coli. Valorization glycerol, main...
Three novel enzyme cascades were engineered for the rapid biosynthesis of phenolic glycosides and their derivatives from biomass-derived<italic>p</italic>-coumaric acid ferulic acid.
The major player for catabolite repression is the phosphotransferase systems (PTSs) and cAMP-Crp. Moreover, Cra controls carbon flow in metabolic network. In present research, effect of modulating cra gene (Δcra) on consumption multiple sources such as glucose fructose (as well xylose) was investigated under both aerobic anaerobic conditions. It shown that could be co-metabolized with consumed faster than mutant also implied higher amount total sources, which contributed to highest...
Abstract Aims Phenazines, such as phenazine-1-carboxylic acid (PCA), phenazine-1-carboxamide (PCN), 2-hydroxyphenazine-1-carboxylic (2-OH-PCA), 2-hydroxyphenazine (2-OH-PHZ), are a class of secondary metabolites secreted by plant-beneficial Pseudomonas. Ps. chlororaphis GP72 utilizes glycerol to synthesize PCA, 2-OH-PCA and 2-OH-PHZ, exhibiting broad-spectrum antifungal activity. Previous studies showed that the addition dithiothreitol (DTT) could increase phenazines production in GP72AN....
Phenazine-1-carboxamide (PCN) has been exploited as a successful biopesticide due to its broad-spectrum antifungal activity. We engineered PCN-overproducing Pseudomonas chlororaphis strain through overexpressing shikimate pathway genes (aroB, aroQ, aroE, and phzC) deleting negative regulatory (relA, fleQ, pykF). The optimized produced 1.92 g/L PCN with yield of 0.11 g/g glycerol, the highest titer ever reported by using minimal media. To gain deeper insights into underlying network, final...