Small extracellular vesicles (sEVs) play a crucial role in intercellular communication and have demonstrated significant relevance reproductive biotechnology, particularly vitro maturation (IVM) bovine embryo production. This study evaluates the effects of follicular fluid-derived (ffsEVs) isolated using two methods: ultracentrifugation (UC) size-exclusion chromatography (SEC) on oocyte preimplantational embryonic development. Significant differences size ffsEVs obtained by both isolation...

Summary The present study was conducted to assess the development, quality and gene expression profile of oxidative stress-related genes bovine embryos cultured in different culture systems with low oxygen tension (5% CO 2 , 5% O 90% N ). assessed included: (1) an incubator chamber; (2) a plastic bag; (3) foil bag. choice system had no effect on cleavage rate at 72 h. However, significant differences ( P < 0.01) were observed blastocysts registered day 7 (29.8, 20.2 12.7% for chamber, bag...

Gamete co-incubation generates high free radical levels surrounding growing zygotes which may impair subsequent embryo viability. Melatonin eliminates a wide variety of radicals; hence, we tried to improve in vitro production by adding melatonin fertilisation (IVF) media (Exp. 1) and low concentrations 2), evaluated its effect on bull sperm function during IVF time 3). In Experiment 1, supplemented culture with 0.01, 0.1 1 mmol melatonin, along no control group. 2, were reduced 10, 100 1000...

Cryopreservation of stallion semen has not reached the level efficiency and positive results described in other species. This is mainly due to greater sensitivity sperm freezing process, showing higher rates oxidative stress plasma membrane damage, which trigger activation several cell damage pathways that ultimately culminate DNA fragmentation death. Therefore, finding molecules improve this technique by preventing required. Thus, aim present study was evaluate effect adding three...

Summary Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a sensitive and accurate tool for estimation of gene levels in preimplantation embryos. To control possible experimental variations, expression data must be normalized using internal genes commonly known as reference genes. However, the stability can vary depending on state development and/or conditions; hence assessment their essential before initiating analysis. In present study, we used RT-qPCR to...

The efficiency of intracytoplasmic sperm injection (ICSI) in the bovine is low compared to other species. It unknown whether defective oocyte activation and/or head decondensation limit success this technique To elucidate where main obstacle lies, we used homologous and heterologous ICSI parthenogenetic procedures. We also evaluated vitro maturation negatively impacted early stages after ICSI. Here showed that injected are resistant nuclear by oocytes only partly overcome exogenous...

Abstract In the present study, we examined effect of two‐step and sequential culture systems on development, quality, gene expression profile bovine embryos generated by in vitro fertilization. Presumptive zygotes were randomly allocated to four treatments: (1) KSOM + 0.4% BSA for 3 days, then 5% FBS day 7 (K‐K/FBS); (2) 0.1% SOF (K‐S/FBS); (3) 0.8% (K‐S/BSA); (4) (K‐K/BSA). Culture medium had no cleavage rate. However, a significant difference ( P < 0.01) was observed with systems,...

The efficiency of intracytoplasmic sperm injection (ICSI) in bovines is lower than other species due, part, to a lack optimal conditions for its implementation; this has hindered the achievement high rates embryonic development and birth live offspring. aim present study was evaluate effects pretreatment bovine spermatozoa with NaOH dithiothreitol (DTT) on viability, plasma membrane integrity, DNA fragmentation vitro developmental potential embryos generated by ICSI. Following 5 mM DTT 20...

SUMMARY We evaluated the potential effect of anisomycin, an antibiotic produced by Streptomyces griseolus , on parthenogenetic activation bovine oocytes and reconstructed somatic‐cell nuclear transfer (SCNT) embryos. A higher cleavage blastocyst rate were achieved with anisomycin (70.3% 27.8%) 6‐dimethylaminopurine (DMAP) (73.3% 30.2%), relative to parthenogenetically activated cycloheximide (CHX) (54.1% 20.2%). In SCNT embryos, a greater proportion embryos reached stage after (32.2%)...

Summary Increasing the efficiency of intracytoplasmic sperm injection (ICSI) in domestic animals has been attempted by many researchers, however embryonic development to blastocyst stage remains low compared with that vitro fertilization (IVF) embryos. One main problems observed cattle is inadequate oocyte activation after ICSI. The present study effect cycloheximide (CHX), 6-dimethylaminopurine (DMAP), and anisomycin (ANY) on rate, development, ploidy quality bovine embryos generated...

Indomethacin is a non-selective NSAID used against pain and inflammation. Although cyclooxygenase (COX) inhibition considered indomethacin’s primary action mechanism, COX-independent ways are associated with beneficial effects in cancer. In colon cancer cells, the activation of peroxisome proliferator-activated receptor-γ (PPAR-γ) related to increase spermidine/spermine-N1-acetyltransferase-1 (SSAT-1), key enzyme for polyamine degradation, cell cycle arrest. increases SSAT-1 levels lung...

The efficiency of intracytoplasmic sperm injection (ICSI) in bovines is low compared to other species due part inadequate egg activation and nucleus decondensation after injection. We hypothesized that this the lack complete capacitation, so we evaluated effects isobutylmethylxanthine (IBMX) methyl-β-cyclodextrin (MβCD) on bovine capacitation preimplantation developmental potential embryos generated by ICSI. Treatment with IBMX MβCD decreased viability (between 13-30%); nevertheless, 0.4 mM...

Excess of reactive oxygen species (ROS) on in vitro embryo production systems negatively affects the quality and developmental potential embryos, as result a decreased sperm increased DNA fragmentation. This issue is major importance assisted fertilisation procedures such intracytoplasmic injection (ICSI), because this technique does not allow natural selection competent spermatozoa, therefore, DNA-damaged spermatozoa might be used to fertilise an egg. The aim study was investigate new...

Contents Mammalian sperm undergo a series of biochemical transformations in the female reproductive tract that are collectively known as capacitation. Cyclodextrins added to culture medium have been described induce vitro capacitation, enabling its use protein‐free media. However, additive capacitating effect methyl‐ β ‐cyclodextrin (M CD ) containing bovine serum albumin ( BSA is unknown species. In this study, we evaluated effects incubating frozen–thawed spermatozoa ‐containing...