A5081457776- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nitric Oxide and Endothelin Effects
- Reproductive System and Pregnancy
- Animal Genetics and Reproduction
- Infectious Disease Case Reports and Treatments
- Selenium in Biological Systems
- Reproductive tract infections research
- Ovarian function and disorders
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- Inflammatory Biomarkers in Disease Prognosis
- Bee Products Chemical Analysis
- Immune cells in cancer
- Reproductive Health and Technologies
- DNA Repair Mechanisms
- Helminth infection and control
- Medical and Biological Ozone Research
- Eosinophilic Disorders and Syndromes
- Organ Transplantation Techniques and Outcomes
- Glutathione Transferases and Polymorphisms
- Toxoplasma gondii Research Studies
Universidad de La Frontera
2015-2024
Université Libre de Bruxelles
1978
Severe acute syndrome coronavirus 2 can invade a variety of tissues, including the testis. Even though this virus is scarcely found in human semen polymerase chain reaction tests, autopsy studies confirm viral presence all testicular cell types, spermatozoa and spermatids.
Abstract STUDY QUESTION Does oxidative stress (OS) activate autophagy in human sperm? SUMMARY ANSWER Human spermatozoa subjected to OS an autophagic response. WHAT IS KNOWN ALREADY Autophagy is a regulated pathway of lysosomal degradation which helps eukaryotic cells maintain or restore homeostasis, being cellular response mechanism. main cause impaired sperm function and linked male infertility; however, whether activates unknown. DESIGN, SIZE, DURATION were exposed separately ionomycin...
In several mammalian species, acute endometritis driven by the recruitment of polymorphonuclear cells (PMN) occurs in response to semen. These PMNs release DNA form neutrophil extracellular traps (NETs) cattle, horse and human, leading sperm entrapment. While there is no evidence this phenomenon occurring donkeys, artificial insemination (AI) with frozen-thawed semen, which results very poor pregnancy rates, leads a large PMN uterus.To investigate whether donkey semen can trigger NET...
Oxidative stress (OS) and disrupted antioxidant defense mechanisms play a pivotal role in the etiology of male infertility. The alterations reactive oxygen species (ROS) production calcium (Ca2+) homeostasis are main activators for mitochondrial permeability transition pore (mPTP) opening. mPTP opening is one involved dysfunction spermatozoa. This alteration function adversely affects energy supply, sperm motility, fertilizing capacity contributes to development In human spermatozoa, has...
The presence of bacteria and/or leukocytes can alter semen quality resulting in low sperm and infertility. Inflammation or infection increases the numbers PMN macrophages/monocytes male genital tract. Release extracellular traps (ETs) by has been recognized as a novel mechanism early host innate immunity, response to invasive pathogens. This is first work that evaluated triggered ETs monocytes co-incubated with spermatozoa effect on function. Selected human isolated from peripheral blood...
Reproductive failure in dogs is often due to unknown causes, and correct diagnosis treatment are not always achieved. This condition associated with various congenital acquired etiologies that develop inflammatory processes, causing an increase the number of leukocytes within female reproductive tract (FRT). An encounter between polymorphonuclear neutrophils (PMNs) infectious agents or inflammation FRT could trigger neutrophil extracellular traps (NETs), which significantly decreased...
The efficiency of intracytoplasmic sperm injection (ICSI) in bovines is low compared to other species due part inadequate egg activation and nucleus decondensation after injection. We hypothesized that this the lack complete capacitation, so we evaluated effects isobutylmethylxanthine (IBMX) methyl-β-cyclodextrin (MβCD) on bovine capacitation preimplantation developmental potential embryos generated by ICSI. Treatment with IBMX MβCD decreased viability (between 13-30%); nevertheless, 0.4 mM...
Contents Mammalian sperm undergo a series of biochemical transformations in the female reproductive tract that are collectively known as capacitation. Cyclodextrins added to culture medium have been described induce vitro capacitation, enabling its use protein‐free media. However, additive capacitating effect methyl‐ β ‐cyclodextrin (M CD ) containing bovine serum albumin ( BSA is unknown species. In this study, we evaluated effects incubating frozen–thawed spermatozoa ‐containing...
Pre-treatment of boar semen with a red light photostimulation procedure increases its "in vivo" fertilising ability. However, vitro" conducted studies shown contradictory results regarding the ability photostimulated spermatozoa to react against strong stress and achieve capacitation status. The aim here was determine effect on response short-term moderate thermal semen. Boar exposed LED regime emitting 620-630 nm during 10 min light, rest after 3 hr since collected. An aliquot without...
Neutrophil extracellular traps (NETs) play a key role in fertilisation by eliminating microorganisms and entrapping spermatozoa the female reproductive tract (FRT). The deleterious effects of NETs on have been previously described; however, individual exposure to NET-derived components bull has not explored. aim this study was evaluate main proteins, histone 2A (H2A), neutrophil elastase (ELA), myeloperoxidase (MPO), pentraxin 3 (PTX), cathepsin G (Cat-G), cathelicidin LL37 (LL-37), at...
Infectious vaginitis is a microbiological syndrome of great importance in public health that affects millions women worldwide. However, no studies have explored the phenomenon production neutrophil extracellular traps (NETs) are released into female reproductive tract these pathologies. This study aimed to determine presence NETosis vaginal discharges with bacterial vaginosis, candidiasis, and trichomoniasis by characterizing NETs. Extracellular DNA elastase citrullinated histones was...
Leucocytospermia has been associated with loss of sperm function. Extracellular traps (ETs) leucocytes are produced during innate immune response. ETs can be activated by spermatozoa in contact polymorphonuclear (in vitro), inducing entrapment and decrease motility. In this pilot study, we describe the results ETosis ex vivo, seminal fluid (SF) smear infertile patients, associating leucocytospermia bacteriospermia. 21 semen parameters (WHO, 2010), microbiological presence SF were determined....
Ferroptosis is a type of iron-dependent regulated cell death characterized by increased bioavailability redox-active iron, loss GPX4 antioxidant capacity, and oxidation polyunsaturated fatty acid-containing phospholipids mediated reactive oxygen species (ROS). The aim this study was to evaluate the effect oxidative stress induced arachidonic acid (AA) on ferroptotic in human spermatozoa.
Trichomoniasis, a globally distributed sexually transmitted infection, is caused by the urogenital parasite Trichomonas vaginalis Donné, 1836 affecting both women and men. The treatment of choice metronidazole (MTZ). In present study, 15 samples vaginal discharge urine were analysed sequencing nitroreductase genes (ntr4 ntr6). An in silico model was structured to illustrate location point mutations (PM) protein. ntr4 gene presented four PMs: G76C (10/10), C213G (9/10), C318A (5/10) G424A...