A5091622388- Erythrocyte Function and Pathophysiology
- Blood properties and coagulation
- Blood groups and transfusion
- Digital Imaging for Blood Diseases
- Hemoglobinopathies and Related Disorders
- Cell Image Analysis Techniques
- Caveolin-1 and cellular processes
- Clusterin in disease pathology
- Blood transfusion and management
- Ion channel regulation and function
- COVID-19 Clinical Research Studies
- Erythropoietin and Anemia Treatment
- Artificial Intelligence in Healthcare and Education
- Surgical Sutures and Adhesives
- Genetic Neurodegenerative Diseases
- Plant Water Relations and Carbon Dynamics
- Platelet Disorders and Treatments
- Autopsy Techniques and Outcomes
- Medical Image Segmentation Techniques
- Lipid metabolism and disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Mitochondrial Function and Pathology
- Lipid Membrane Structure and Behavior
- Hemostasis and retained surgical items
- High Altitude and Hypoxia
Saarland University
2019-2024
Universitätsklinikum des Saarlandes
2021
Glutaraldehyde is a well-known substance used in biomedical research to fix cells. Since haemolytic anaemias are often associated with red blood cell shape changes deviating from the biconcave disk shape, conservation of these shapes for imaging general and 3D-imaging particular, like confocal microscopy, scanning electron microscopy or probe common desire. Along fixation comes an increase stiffness In context cells this increased rigidity mimic malaria infected because they also stiffer...
The Gárdos channel (KCNN4) and Piezo1 are the best-known ion channels in red blood cell (RBC) membrane. Nevertheless, quantitative electrophysiological behavior of RBCs its heterogeneity still not completely understood. Here, we use state-of-the-art biochemical methods to probe for abundance RBCs. Furthermore, utilize automated patch clamp, based on planar chips, compare activity two reticulocytes mature In addition this characterization, performed membrane potential measurements demonstrate...
Abstract Aims Total haemoglobin mass (tot‐Hb) increases during high‐altitude acclimatization. Normalization of tot‐Hb upon descent is thought to occur via neocytolysis, the selective destruction newly formed erythrocytes. Because convincing experimental proof neocytolysis lacking, we performed a prospective study on erythrocyte survival after stay at Jungfraujoch Research Station (JFJRS; 3450 m). Methods Newly erythrocytes 12 male subjects (mean age 23.3 years) were cohort labelled in...
Coronavirus disease 2019 (COVID-19) is caused by the Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) and can affect multiple organs, among which circulatory system. Inflammation mortality risk markers were previously detected in COVID-19 plasma red blood cells (RBCs) metabolic proteomic profiles. Additionally, biophysical properties, such as deformability, found to be changed during infection. Based on data, we aim better characterize RBC functions COVID-19. We evaluate flow properties of...
In many medical disciplines, red blood cells are discovered to be biomarkers since they "experience" various conditions in basically all organs of the body. Classical examples diabetes and hypercholesterolemia. However, recently cell distribution width (RDW), is often referred to, as an unspecific parameter/marker (e.g., for cardiac events or oncological studies). The measurement RDW requires venous samples perform complete count (CBC). Here, we introduce Erysense, a lab-on-a-chip-based...
The distribution of red blood cells (RBCs) in the microcirculation determines oxygen delivery and solute transport to tissues. This process relies on partitioning RBCs at successive bifurcations throughout microvascular network, it has been known since last century that partition disproportionately fractional flow rate, therefore leading heterogeneity hematocrit (i.e., volume fraction blood) microvessels. Usually, downstream a bifurcation, vessel branch with higher receives an even RBC flux....
The investigation of cell shapes mostly relies on the manual classification 2D images, causing a subjective and time consuming evaluation based portion surface. We present dual-stage neural network architecture for analyzing fine shape details from confocal microscopy recordings in 3D. system, tested red blood cells, uses training data both healthy donors patients with congenital disease, namely hereditary spherocytosis. Characteristic features are revealed spherical harmonics spectrum each...
(1) Background: Chorea-acanthocytosis and McLeod syndrome are the core diseases among group of rare neurodegenerative disorders called neuroacanthocytosis syndromes (NASs). NAS patients have a variable number irregularly spiky erythrocytes, so-called acanthocytes. Their detection is crucial but error-prone parameter in diagnosis NASs, often leading to misdiagnoses. (2) Methods: We measured standard Westergren erythrocyte sedimentation rate (ESR) various blood samples from healthy controls....
Background: Chorea-acanthocytosis (ChAc) is a rare hereditary neurodegenerative disease with deformed red blood cells (RBCs), so-called acanthocytes, as typical marker of the disease. Erythrocyte sedimentation rate (ESR) was recently proposed diagnostic biomarker. To date, there no treatment option for affected patients, but promising therapy candidates, such dasatinib, Lyn-kinase inhibitor, have been identified. Methods: RBCs two ChAc patients during and after dasatinib were characterized...
Blood flow in the microcirculatory system is crucially affected by intrinsic red blood cell (RBC) properties, such as their deformability. In smallest vessels of this network, RBCs adapt shapes to conditions. Although it known that age modifies physical increased cytosol viscosity and altered viscoelastic membrane evolution shape-adapting abilities during senescence remains unclear. study, we investigated effect RBC properties on microcapillary vitro behavior characteristic microfluidic...
Abstract An increase in abundance and activity of N‐methyl D‐aspartate receptors (NMDAR) was previously reported for red blood cells (RBCs) sickle cell disease (SCD) patients. Increased Ca 2+ uptake through the receptor supported dehydration RBC damage. In a pilot phase IIa‐b clinical trial MemSID, memantine, blocker NMDAR, used treatment four patients 12 months. Two more that have enrolled into study did not finish it. One them had psychotic event following involuntary overdose drug,...
Hypoxia is associated with increased erythropoietin (EPO) release to drive erythropoiesis. At high altitude, EPO levels first increase and then decrease, although erythropoiesis remains elevated at a stable level. The roles of hypoxia related adjustments are not fully understood, which has contributed the formulation theory neocytolysis. We aimed evaluate role oxygen exclusively on erythropoiesis, comparing in vitro erythroid differentiation performed atmospheric oxygen, lower concentration...
Very young red blood cells, namely reticulocytes, can be quite easily recognised and labelled by cluster of differentiation antibodies (CD71, transferrin receptor) or staining remnant RNA with thiazol orange. In contrast age specific erythrocyte labelling is more difficult in later periods their life time. While erythrocytes contain band 4.1 protein, a molecular clock, so far it has not been possible to read this clock on individual cells. One concept track during time mark them when they...
(1) Background: The Gárdos channel (KCNN4) and Piezo1 are the best-known ion channels in red blood cell (RBC) membrane. Nevertheless, quantitative electrophysiological behavior of RBCs its heterogeneity still not completely understood.; (2) Methods: Here we use state-of-the-art biochemical methods to probe for abundance RBCs. Furthermore, utilize automated patch-clamp, based on planar chips, compare activity two reticulocytes mature Besides this characterization, performed membrane potential...
OPINION article Front. Physiol., 10 June 2021Sec. Red Blood Cell Physiology https://doi.org/10.3389/fphys.2021.690604
We present a dual-stage neural network architecture for analyzing fine shape details from microscopy recordings in 3D. The system, tested on red blood cells, uses training data both healthy donors and patients with congenital disease. Characteristic features are revealed the spherical harmonics spectrum of each cell automatically processed to create reproducible unbiased recognition classification diagnostic theragnostic use.
ABSTRACT Chorea-acanthocytosis (ChAc) and McLeod syndrome (MLS) are the core diseases among group of rare neurodegenerative disorders that comprise neuroacanthocytosis (NAS). Both ChAc MLS patients present with an increased number irregularly spiky erythrocytes, so-called acanthocytes. The detection acanthocytes is often a crucial parameter in diagnosis NAS. However, this approach error-prone not very reliable, typically explaining misdiagnosis NAS patients. Based on standard Westergren...
ABSTRACT Coronavirus disease 2019 (COVID-19) is caused by the Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) and can affect multiple organs, among which circulatory system. Inflammation mortality risk markers were previously detected in COVID-19 plasma red blood cells (RBCs) metabolic proteomic profiles. Additionally, biophysical properties, such as deformability, found to be changed during infection. Based on data, we aim better characterize RBC functions COVID-19. We evaluate flow...
ZUSAMMENFASSUNG Neozytolyse bezeichnet die bevorzugte Zerstörung von jungen roten Blutzellen (Neozyten), wenn zu viele Erythrozyten vorhanden sind. Das kommt in der Raumfahrt oder beim Abstieg nach langem Höhenaufenthalt vor. Mit dem Ziel, Existenz erstmals beweisen, haben wir Forschungsstation Jungfraujoch (3450 Hm) eine prospektive Studie durchgeführt. Neu gebildete 12 männlichen Probanden wurden durch Einnahme nichtradioaktivem Glyzin mit seltenen Isotopen markiert: Heidelberg (110 und...
Abstract The distribution of red blood cells (RBCs) in the microcirculation determines oxygen delivery and solute transport to tissues. This process relies on partitioning RBCs at successive bifurcations throughout microvascular network it is known since last century that partition disproportionately fractional flow rate, therefore leading heterogeneity hematocrit (i.e. volume fraction blood) microvessels. Usually, downstream a bifurcation, vessel branch with higher receives an even RBC...