A5057582349- Erythrocyte Function and Pathophysiology
- Blood properties and coagulation
- Lipid Membrane Structure and Behavior
- Hemoglobin structure and function
- Pancreatic function and diabetes
- Neonatal Health and Biochemistry
- Sulfur Compounds in Biology
- Diabetes and associated disorders
- Nitric Oxide and Endothelin Effects
- Erythropoietin and Anemia Treatment
- Nanopore and Nanochannel Transport Studies
- Neuroscience of respiration and sleep
- Hemoglobinopathies and Related Disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Redox biology and oxidative stress
- High Altitude and Hypoxia
- Blood groups and transfusion
- Selenium in Biological Systems
- Caveolin-1 and cellular processes
- Trace Elements in Health
- Digital Imaging for Blood Diseases
- Boron Compounds in Chemistry
- Advanced Glycation End Products research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Xenotransplantation and immune response
University of Pavia
2016-2025
Swiss Integrative Center for Human Health
2023
University of Zurich
2023
European Commission
2010
Purdue University West Lafayette
1997
Abstract In the age of “omics”, lipidomics erythropoiesis is still missing. How reticulocytes mature in circulation into functional erythrocytes also largely unknown. We have isolated here two populations human circulating at different levels maturation, and three subpopulations age, characterized evolution their lipidome. (Sphingomyelin+cholesterol) partly phosphatidylethanolamine increase relative to total lipids, whereas phosphatidylcholine phosphatidylserine decrease from immature...
Glutaraldehyde is a well-known substance used in biomedical research to fix cells. Since haemolytic anaemias are often associated with red blood cell shape changes deviating from the biconcave disk shape, conservation of these shapes for imaging general and 3D-imaging particular, like confocal microscopy, scanning electron microscopy or probe common desire. Along fixation comes an increase stiffness In context cells this increased rigidity mimic malaria infected because they also stiffer...
Aging is a complicate process that far away from being understood but increasingly investigated. The term majorly used for multicellular organisms because singe cells, especially cell lines in laboratories, are often immortal. Nevertheless, also based on single cells. Therefore, the understanding of how age requires cells age. In recent years and with progress single-cell molecular biology, certain genetic determination aging transcriptional signatures patterns were recognized, example.1...
Abstract Aims Total haemoglobin mass (tot‐Hb) increases during high‐altitude acclimatization. Normalization of tot‐Hb upon descent is thought to occur via neocytolysis, the selective destruction newly formed erythrocytes. Because convincing experimental proof neocytolysis lacking, we performed a prospective study on erythrocyte survival after stay at Jungfraujoch Research Station (JFJRS; 3450 m). Methods Newly erythrocytes 12 male subjects (mean age 23.3 years) were cohort labelled in...
Fast changes in environmental oxygen availability translate into shifts mitochondrial free radical production. An increase intraerythrocytic reduced glutathione (GSH) during deoxygenation would support the detoxification of exogenous oxidants released circulation from hypoxic peripheral tissues. Although reported, mechanism behind this acute oxygen-dependent regulation GSH red blood cells remains unknown. This study explores role hemoglobin (Hb) modulation levels cells. We have demonstrated...
Red blood cell (RBC) metabolic reprogramming upon exposure to high altitude contributes physiological human adaptations hypoxia, a multifaceted process critical health and disease. To delve into the molecular underpinnings of this phenomenon, first, we performed multi-omics analysis RBCs from six lowlanders after high-altitude with longitudinal sampling at baseline, ascent 5,100 m descent sea level. Results highlighted an association between erythrocyte levels 2,3-bisphosphoglycerate (BPG),...
The pursuit of ex vivo erythrocyte generation has led to the development various culture systems that simulate bone marrow microenvironment. However, these models often fail fully replicate hematopoietic niche's complex dynamics. In our research, we employ a comprehensive strategy emphasizes physiological red blood cell (RBC) differentiation using minimal cytokine regimen. A key innovation in approach is integration three-dimensional (3D) silk-based scaffold engineered mimic both physical...
Background/Aims: A high surface-to-volume ratio and a spectrin membrane-skeleton (MS) confer to the mammalian red blood cells (RBCs) their characteristic deformability, mechanical strength structural stability. During 120 days of circulatory life in humans, RBCs decrease size, while remaining biconcave disks, owing coordinated membrane surface area cell water. It is generally believed that part lost with shedding spectrin-free vesicles same type can be obtained vitro by different treatments....
Human erythrocytes were induced to release membrane vesicles by treatment with Ca2+ and ionophore A23187. In addition the biochemical changes already known accompany loading of human Ca2+, present study reveals that tyrosine phosphorylation anion exchanger band 3 protein also occurs. The relationship between vesiculation was analysed using quinine (a non-specific inhibitor Ca(2+)-activated K+ channel, only Ca(2+)-induced vesiculation) charybdotoxin, a specific apamin-insensitive...
Abstract Boronic acids and their esters are highly considered compounds for the design of new drugs drug delivery devices, particularly as boron-carriers suitable neutron capture therapy. However, these only marginally stable in water. Hydrolysis some phenylboronic pinacol is described here. The kinetics dependent on substituents aromatic ring. Also pH strongly influences rate reaction, which considerably accelerated at physiological pH. Therefore, care must be taken when considering boronic...
Background: Old human red blood cells (RBCs) have a reduced surface area with respect to young RBCs. If this decrease occurred through the release of vesicles similar spectrin-free that are shed in vitro under different experimental conditions or during storage, there would be no membrane-skeleton, but only lipid bilayer area, RBC ageing vivo. However, we observed spectrin and other membrane-skeletal proteins old Because RBCs contain components ubiquitin-proteasome system hydrolytic systems...
Human erythrocyte band 3 becomes rapidly phosphorylated on tyrosine residues after exposure of erythrocytes to hypertonic conditions. The driving force for this phosphorylation reaction seems be a decrease in cell volume, because (1) changes phosphotyrosine content accurately track repeated volume through several cycles swelling and shrinking; (2) the level is independent osmolyte employed but strongly sensitive magnitude shrinkage; (3) buffers under conditions which intracellular osmolarity...
One of the most intensively studied post-translational modifications erythrocyte proteins is phosphorylation tyrosine residues band 3, which strictly regulated in vivo by PTKs (protein-tyrosine kinases) and PTPs (protein-phosphotyrosine phosphatases). Two (p72(syk) p56/53(lyn)) two PTP activities (PTP1B SHPTP-2) have been immunologically identified so far mature human erythrocytes. We shown previously that 3 undergoes upon a decrease cell volume, as occurs when erythrocytes treated with...