A5100673414- Neuroscience and Neuropharmacology Research
- Cerebrospinal fluid and hydrocephalus
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Traumatic Brain Injury and Neurovascular Disturbances
- Ion channel regulation and function
- Wnt/β-catenin signaling in development and cancer
- Plant Stress Responses and Tolerance
- Fetal and Pediatric Neurological Disorders
- Plant-Microbe Interactions and Immunity
- Spinal Dysraphism and Malformations
- Genetics and Neurodevelopmental Disorders
- Retinal Development and Disorders
- Photosynthetic Processes and Mechanisms
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- ATP Synthase and ATPases Research
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Advanced Polymer Synthesis and Characterization
- Neurological and metabolic disorders
- MicroRNA in disease regulation
- 14-3-3 protein interactions
- Plant tissue culture and regeneration
- Cell death mechanisms and regulation
Gangneung–Wonju National University
2024
Medical College of Wisconsin
2007-2023
Ulsan College
2021
University of Miami
2014-2021
University of Ulsan
2021
Asan Medical Center
2021
Keimyung University
2003-2019
Keimyung University Dongsan Medical Center
2019
Institute of Pharmacology
2015
Korea Institute of Science and Technology
2015
Ca(2+)-calmodulin-dependent protein kinase II (CaMKII) is a serine/threonine critically involved in synaptic plasticity the brain. It highly concentrated postsynaptic density fraction, exceeding amount of any other signal transduction molecules. Because signaling can be amplified by catalytic reaction, why CaMKII exists such large quantity has been mystery. Here, we provide biochemical evidence that capable bundling F-actin through stoichiometric interaction. Consistent with this evidence,...
Cold stress on plants induces changes in the transcription of cold response genes. A cDNA clone encoding C2H2-type zinc finger protein, SCOF-1, was isolated from soybean. The SCOF-1 is specifically induced by low temperature and abscisic acid (ABA) but not dehydration or high salinity. Constitutive overexpression cold-regulated (COR) gene expression enhanced tolerance non-acclimated transgenic Arabidopsis tobacco plants. localized to nucleus did bind directly either C-repeat/dehydration...
To develop a noninvasive method for intracranial elastance and pressure (ICP) measurement.Intracranial volume changes were calculated from magnetic resonance (MR) imaging measurements of cerebrospinal fluid (CSF) blood flow. The change was the net transcranial CSF volumetric flow rates. in derived gradient velocity. An index ratio to change. reproducibility measurement established four five healthy volunteers. measured compared with invasive ICP patients an intraventricular catheter at MR...
The Ca2+ signal is essential for the activation of plant defense responses, but downstream components signaling pathway are still poorly defined. Here we demonstrate that specific calmodulin (CaM) isoforms activated by infection or pathogen-derived elicitors and participate in Ca2+-mediated induction disease resistance responses. Soybean CaM (SCaM)-4 SCaM-5 genes, which encode divergent isoforms, were induced within 30 min a fungal elicitor pathogen, whereas other SCaM genes encoding highly...
Abstract Purpose To quantify the effect of posture on intracranial physiology in humans by MRI, and demonstrate relationship between compliance (ICC) pressure (ICP), pulsatility blood CSF flows. Materials Methods Ten healthy volunteers (29 ± 7 years old) were scanned supine sitting positions using a vertical gap MRI scanner. Pulsatile flows into out from brain visualized quantified time‐of‐flight (TOF) cine phase‐contrast techniques, respectively. The total cerebral flow (tCBF), venous...
Calmodulin plays pivotal roles in the transduction of various Ca2+-mediated signals and is one most highly conserved proteins eukaryotic cells. In plants, multiple calmodulin isoforms with minor amino acid sequence differences were identified but their functional significances are unknown. To investigate biological function calmodulins regulation calmodulin-dependent enzymes, we cloned cDNAs encoding soybean. Among five isolated from soybean, designated as SCaM-1 to −5, SCaM-4 −5 encoded...
Transient influx of Ca2+ constitutes an early event in the signaling cascades that trigger plant defense responses. However, downstream components defense-associated are largely unknown. Because signals mediated by Ca2+-binding proteins, including calmodulin (CaM), identification and characterization CaM-binding proteins elicited pathogens should provide insights into mechanism which regulates In this study, we isolated a gene encoding rice Mlo (Oryza sativa Mlo;OsMlo) using protein-protein...
To determine the role of prostacyclin (PGI2) in protecting endothelial cells (ECs) from apoptosis and elucidate protective mechanism.To evaluate effect PGI2 on EC survival, we treated ECs with Ad-COX1/PGIS (Ad-COPI), which augmented selectively production or carbaprostacyclin (cPGI2) followed by H2O2 for 4 hours. Ad-COPI inhibited annexin V-positive blocked caspase 3 activation. cPGI2 a concentration-dependent manner. L-165041 had similar effect, suggesting involvement peroxisome...
To assess the effects of low-dose acetazolamide treatment on volumetric MRI markers and clinical outcome in idiopathic normal-pressure hydrocephalus (iNPH).We analyzed gait measures from 8 patients with iNPH serial MRIs an institutional review board-approved imaging protocol who had been treated off-label (125-375 mg/day). studies included fluid-attenuated inversion recovery 3D T1-weighted high-resolution imaging. Automated analyses were employed to quantify each patient's ventricular,...
Ca2+-ATPases are key regulators of Ca2+ ion efflux in all eukaryotes. Animal cells have two distinct families pumps, with calmodulin-stimulated pumps (type IIB pumps) found exclusively at the plasma membrane. In plants, no equivalent type pump located membrane has been identified molecular level, although related isoforms non–plasma locations. Here, we identify a plant cDNA, designated SCA1 (for soybean Ca2+-ATPase 1), that encodes and is The localization was determined by sucrose gradient...
alpha-Amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)-type glutamate receptors undergo constitutive and ligand-induced internalization that requires dynamin the clathrin adaptor complex AP-2. We report here an atypical basic motif within cytoplasmic tails of AMPA-type directly associates with mu2-adaptin by a mechanism similar to recognition presynaptic vesicle protein synaptotagmin 1 A 1-derived AP-2 binding peptide competes interaction AMPA receptor subunit GluR2 AP-2mu increases...
Continuous modification of the protein composition at synapses is a driving force for plastic changes synaptic strength, and provides fundamental molecular mechanism plasticity information storage in brain. Studying turnover not only important understanding learning memory, but also has direct implication pathological conditions like aging, neurodegenerative diseases, psychiatric disorders. Proteins involved transmission are typically concentrated neurons thus appear as puncta (clusters)...
Abstract Secreted Wnts play crucial roles in synaptogenesis and synapse maintenance, but endogenous factors promoting elimination central neurons remain unknown. Here we show that proline-rich 7 (PRR7) induces specific removal of excitatory synapses acts as a Wnt inhibitor. Remarkably, transmembrane protein PRR7 is activity-dependently released by via exosomes. Exosomal uptaken through membrane fusion eliminates neighboring neurons. Conversely, knockdown sparse greatly increases numbers all...
The Rap family of small GTPases is implicated in the mechanisms synaptic plasticity, particularly depression. Here we studied role neuronal morphogenesis and transmission cultured neurons. Constitutively active Rap2 expressed hippocampal pyramidal neurons caused decreased length complexity both axonal dendritic branches. In addition, loss spines spiny synapses, an increase filopodia-like protrusions shaft synapses. These morphological effects were absent aspiny interneurons. contrast,...