A5074206429- Hearing, Cochlea, Tinnitus, Genetics
- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Hearing Loss and Rehabilitation
- Parvovirus B19 Infection Studies
- MicroRNA in disease regulation
- Molecular Communication and Nanonetworks
- Heat shock proteins research
- Neonatal and Maternal Infections
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Developmental Biology and Gene Regulation
- Neurogenesis and neuroplasticity mechanisms
- Abdominal Trauma and Injuries
- Neonatal Respiratory Health Research
- Diverticular Disease and Complications
- Neuroinflammation and Neurodegeneration Mechanisms
- Olfactory and Sensory Function Studies
- Abdominal vascular conditions and treatments
- RNA Interference and Gene Delivery
- Yersinia bacterium, plague, ectoparasites research
- Genital Health and Disease
- Connexins and lens biology
- Microbial infections and disease research
- IgG4-Related and Inflammatory Diseases
- Soft tissue tumors and treatment
University of Bern
2015-2020
University Hospital of Bern
2015-2020
Abstract Sensory hair cells located in the organ of Corti are essential for cochlear mechanosensation. Their loss is irreversible humans resulting permanent hearing loss. The development therapeutic interventions requires fundamental knowledge about similarities and potential differences between animal models human as well establishment cell based-assays. Here we analyze gene protein expression developing inner ear a temporal window spanning from week 8 to 12 post conception, when become...
Hearing loss is an important sequela of pneumococcal meningitis (PM), occurring in up to 30% survivors. The role the severity infection on hearing function and pathomorphological consequences cochlea secondary PM have not been investigated date. Using a well-established model PM, we systematically functional outcome long-term fate neurosensory cells cochlea, i.e., hair spiral ganglion neurons (SGNs), with focus their tonotopic distribution. Intracisternal infant rats increasing inocula...
Age-related hearing (ARHL) loss affects a large part of the human population with major impact on our aging societies. Yet, underlying mechanisms are not understood, and no validated therapy or prevention exists. NADPH oxidases (NOX), important sources reactive oxygen species (ROS) in cochlea might therefore be involved pathogenesis ARHL. Here we investigate ARHL mouse model. Wild type mice showed early cochlear integrity, while animals deficient NOX subunit p22phox remained unaffected up to...
The peripheral hearing process taking place in the cochlea mainly depends on two distinct sensory cell types: mechanosensitive hair cells and spiral ganglion neurons. first respond to mechanical stimulation exerted by sound pressure waves their bundles releasing neurotransmitters thereby activating latter. Loss of these sensorineural is associated with permanent loss. Stem cell-based approaches aiming at replacement or vitro drug testing identify potential ototoxic, otoprotective...
Pneumococcal meningitis is associated with high mortality and morbidity rates. Up to 50% of survivors show neurologic sequelae including hearing loss, cognitive impairments learning disabilities, being particularly detrimental in affected infants children where adjuvant therapy dexamethasone has no proven beneficial effect. We evaluated the effect concomitantly targeting specific pathophysiological mechanisms responsible for brain damage—i.e. matrix-metalloproteinase (MMP) activity...
Abstract Due to the lack of regenerative capacity mammalian auditory epithelium, sensory hair cell loss results in permanent hearing deficit. Nevertheless, a population tissue resident stem/progenitor cells has been recently described. Identification methods trigger their activity could lead exploitation potential therapeutically. Here we validate use transgenic mice reporting cycle progression (FUCCI) and stemness (Lgr5-GFP), as valuable tool identify regulators re-entry supporting within...
Despite appropriate antibiotic therapy, pneumococcal meningitis (PM) is associated with a case fatality rate of up to 30% in high-income countries. Survivors often suffer from severe lifelong disabilities.
Abstract Channelrhodopsin-2 (ChR2) has become a celebrated research tool and is considered promising potential therapeutic for neurological disorders. While making its way into the clinic, concerns about safety of chronic ChR2 activation have emerged; in particular as high-intensity blue light illumination needed may be phototoxic. Here we set out to quantify first time cytotoxic effects activation. We studied prolonged on ChR2(D156A)-expressing human melanoma cells cancer are notorious...
Sensorineural hearing loss is the most common long-term deficit after pneumococcal meningitis (PM), occurring in up to 30% of surviving patients. The infection and following overshooting inflammatory host response damage vulnerable sensory cells inner ear, resulting hair spiral ganglion neurons, ultimately leading elevated thresholds. Here, we tested oto-protective properties small heat shock protein alpha B-crystallin (HspB5) with previously reported anti-inflammatory, anti-apoptotic...
Hearing loss remains the most common long-term complication of pneumococcal meningitis (PM) reported in up to 30% survivors. Streptococcus pneumoniae have been shown possess different ototoxic properties. Here we present a novel ex vivo experimental setup examine detail pattern hair cell upon exposure S. strains, therefore recapitulating pathogen derived aspects PM-induced hearing loss. Our results show higher susceptibility towards pneumoniae-induced cochlear damage for outer cells (OHC)...