A5038368134- Hearing, Cochlea, Tinnitus, Genetics
- Neuroscience and Neural Engineering
- Hearing Loss and Rehabilitation
- Conducting polymers and applications
- Pluripotent Stem Cells Research
- PI3K/AKT/mTOR signaling in cancer
- Protein Kinase Regulation and GTPase Signaling
- Tissue Engineering and Regenerative Medicine
- Single-cell and spatial transcriptomics
- Neurogenesis and neuroplasticity mechanisms
- 3D Printing in Biomedical Research
- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Olfactory and Sensory Function Studies
- Bacterial Infections and Vaccines
- Marine animal studies overview
- Advanced Memory and Neural Computing
- Advanced biosensing and bioanalysis techniques
- Electrochemical Analysis and Applications
- Mast cells and histamine
- Pneumonia and Respiratory Infections
- Metabolism, Diabetes, and Cancer
- Neural dynamics and brain function
- Mesenchymal stem cell research
- Nerve injury and regeneration
University of Zurich
2020-2024
University Hospital of Zurich
2019-2023
University of Bern
2015-2021
StemCells (United States)
2021
University Hospital of Bern
2014-2020
École Polytechnique Fédérale de Lausanne
2011-2019
Bioengineering (Switzerland)
2012-2019
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2014
In-Q-Tel
2012
University Medical Center Utrecht
2002-2010
During the evolution of metazoans and rise systemic hormonal regulation, insulin-controlled class 1 phosphatidylinositol 3OH-kinase (PI3K) pathway was merged with primordial amino acid-driven mammalian target rapamycin (mTOR) to control growth development organism. Insulin regulates mTOR function through a recently described canonical signaling pathway, which is initiated by activation PI3K. However, how acid input integrated that insulin unclear. Here we used number molecular, biochemical,...
The mammalian target of rapamycin (mTOR) assembles a signaling network essential for the regulation cell growth, which has emerged as major anticancer therapies. tuberous sclerosis complex 1 and 2 (TSC1/2) proteins their target, small GTPase Rheb, constitute key regulatory pathway upstream mTOR. Phospholipase D (PLD) its product phosphatidic acid are also regulators mitogenic mTOR signaling. However, how TSC/Rheb PLD pathways interact or integrate in rapamycin-sensitive not been examined...
Stem cell self-renewal, commitment and reprogramming rely on a poorly understood coordination of cycle progression execution fate choices. Using existing experimental paradigms, it has not been possible to probe this relationship systematically in live stem cells vitro or vivo. Alterations kinetics probably occur long before changes phenotypic markers are apparent could be used as predictive parameters reveal fate. To explore intriguing concept, we developed single-cell tracking approach...
Abstract Sensory hair cells located in the organ of Corti are essential for cochlear mechanosensation. Their loss is irreversible humans resulting permanent hearing loss. The development therapeutic interventions requires fundamental knowledge about similarities and potential differences between animal models human as well establishment cell based-assays. Here we analyze gene protein expression developing inner ear a temporal window spanning from week 8 to 12 post conception, when become...
An enzymatically cross-linked polyethylene glycol (PEG)-based hydrogel was engineered to promote and align nerve cells in a three-dimensional manner. To render the injectable, otherwise bioinert, PEG-based material supportive for cell growth, its mechanical biochemical properties were optimized. A recombinant fibronectin fragment (FNIII9*-10/12-14) coupled PEG backbone during gelation provide adhesive growth factor binding domains close vicinity. Compared full-length fibronectin,...
ABSTRACT Our molecular understanding of the early stages human inner ear development has been limited by difficulty in accessing fetal samples at gestational stages. As an alternative, previous studies have shown that morphogenesis can be partially recapitulated using induced pluripotent stem cells directed to differentiate into organoids (IEOs). Once validated and benchmarked, these systems could represent unique tools complement refine our otic differentiation model developmental defects....
Hearing loss is an important sequela of pneumococcal meningitis (PM), occurring in up to 30% survivors. The role the severity infection on hearing function and pathomorphological consequences cochlea secondary PM have not been investigated date. Using a well-established model PM, we systematically functional outcome long-term fate neurosensory cells cochlea, i.e., hair spiral ganglion neurons (SGNs), with focus their tonotopic distribution. Intracisternal infant rats increasing inocula...
Age-related hearing (ARHL) loss affects a large part of the human population with major impact on our aging societies. Yet, underlying mechanisms are not understood, and no validated therapy or prevention exists. NADPH oxidases (NOX), important sources reactive oxygen species (ROS) in cochlea might therefore be involved pathogenesis ARHL. Here we investigate ARHL mouse model. Wild type mice showed early cochlear integrity, while animals deficient NOX subunit p22phox remained unaffected up to...
Abstract Inner ear organoids derived from differentiation of human pluripotent stem cells have recently gained momentum as tools to study inner development and developmental defects. An additional exciting aspect about this technology is represented by its translational potential, specifically, the use validate therapeutics for hearing balance restoration on human/patient-specific cells. This latter will be briefly discussed here including opportunities current limitations.
The peripheral hearing process taking place in the cochlea mainly depends on two distinct sensory cell types: mechanosensitive hair cells and spiral ganglion neurons. first respond to mechanical stimulation exerted by sound pressure waves their bundles releasing neurotransmitters thereby activating latter. Loss of these sensorineural is associated with permanent loss. Stem cell-based approaches aiming at replacement or vitro drug testing identify potential ototoxic, otoprotective...
G protein-coupled receptor kinase (GRK) 2 plays a crucial role in regulating the extent of desensitization and resensitization receptors (GPCRs). We have shown that expression level GRK2 lymphocytes decreases during inflammatory diseases such as arthritis. Reactive oxygen species play an important variety conditions, including demonstrate herein oxidative stress, induced by exposure to H<sub>2</sub>O<sub>2</sub>, results 50% reduction protein levels GRK activity with no changes mRNA...
Journal Article High-throughput clonal analysis of neural stem cells in microarrayed artificial niches Get access Marta Roccio, Roccio School Life Sciences, Institute Bioengineering and Laboratory Stem Cell Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland. Fax: +41 (0)21 693 96 65; Tel: 18 76 Search for other works by this author on: Oxford Academic Google Scholar Samy Gobaa, Gobaa Matthias P. Lutolf E-mail: matthias.lutolf@epfl.ch Integrative Biology, Volume 4,...
Abstract Due to the lack of regenerative capacity mammalian auditory epithelium, sensory hair cell loss results in permanent hearing deficit. Nevertheless, a population tissue resident stem/progenitor cells has been recently described. Identification methods trigger their activity could lead exploitation potential therapeutically. Here we validate use transgenic mice reporting cycle progression (FUCCI) and stemness (Lgr5-GFP), as valuable tool identify regulators re-entry supporting within...
In the past years, cardiovascular progenitor cells have been isolated from human heart and characterized. Up to date, no studies reported in which developmental potential of foetal adult progenitors was tested simultaneously. However, intrinsic differences will likely affect interpretations regarding cell application for regenerative medicine. Here we report a direct comparison between heart-derived cardiomyocyte (CMPCs). We show that CMPCs distinct preferences differentiate into mesodermal...
While cell sorting usually relies on cell-surface protein markers, molecular beacons (MBs) offer the potential to sort cells based presence of any expressed mRNA and in principle could be extremely useful rare populations from primary isolates. We show here how stem can purified mixed by MBs. Specifically, we designed targeting Sox2, a well-known marker for murine embryonic (mES) neural (NSC). One our displayed an increase fluorescence compared nonspecific beacon both vitro vivo when tested...