A5010199055- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- interferon and immune responses
- Multiple Sclerosis Research Studies
- T-cell and B-cell Immunology
- Inflammatory Bowel Disease
- Immune responses and vaccinations
- Immunotherapy and Immune Responses
- Pluripotent Stem Cells Research
- Monoclonal and Polyclonal Antibodies Research
- Blood groups and transfusion
- Ocular Diseases and Behçet’s Syndrome
- vaccines and immunoinformatics approaches
- ATP Synthase and ATPases Research
- Cell Adhesion Molecules Research
- Molecular Biology Techniques and Applications
- Childhood Cancer Survivors' Quality of Life
- Toxoplasma gondii Research Studies
- RNA Research and Splicing
- Skin and Cellular Biology Research
- Cellular Mechanics and Interactions
- Polyomavirus and related diseases
- SARS-CoV-2 detection and testing
- Bioinformatics and Genomic Networks
- Biosensors and Analytical Detection
University of California, Berkeley
2020-2021
QB3
2021
University of California, San Francisco
2008-2014
Yale University
2006
Clinically isolated syndrome (CIS) refers to the earliest clinical manifestation of multiple sclerosis (MS). Currently there are no prognostic biological markers that accurately predict conversion CIS clinically definite MS (CDMS). Furthermore, molecular events in still unknown. We used microarrays study gene expression naïve CD4(+) T cells from 37 patients at time diagnosis and after 1 year. Supervised machine-learning methods were build predictive models disease conversion. identified 975...
Multiple sclerosis (MS) is the most common cause of chronic neurologic disability beginning in early to middle adult life. Results from recent genome-wide association studies (GWAS) have substantially lengthened list disease loci and provide convincing evidence supporting a multifactorial polygenic model inheritance. Nevertheless, knowledge MS genetics remains incomplete, with many risk alleles still be revealed.We used discovery GWAS dataset (8,844 samples, 2,124 cases 6,720 controls)...
The development of a California-based induced pluripotent stem cell (iPSC) bank based on human leukocyte antigen (HLA) haplotype matching represents significant challenge and valuable opportunity for the advancement regenerative medicine. However, previously published models iPSC banks have neither addressed admixed nature populations like that California nor evaluated benefit to population as whole. We developed new model evaluating an haplobank demographic immunogenetic characteristics...
Some breast cancer cases in our previous immunohistochemical studies show Met expression the nucleus. Given nuclear localization of other receptor tyrosine kinases, we proceeded to investigate Met. Nuclear is seen numerous cell lines and germinal regions many tissues using four unique antibodies. Cell fractionation reveals a 60-kDa band recognized by COOH-terminal antibodies that present independent hepatocyte growth factor treatment. Green fluorescent protein (GFP) fusion proteins...
Alpha-catenin functions to anchor adherens junctions the filamentous actin (F-actin) cytoskeleton, through direct and indirect binding mechanisms. When truncated at amino acid 865, alpha-catenin exhibited a markedly reduced F-actin affinity compared wild-type. Expression of mutant in α-catenin deficient colon carcinoma cell line, Clone A, could not restore an adhesive phenotype when compared. Furthermore, fusion protein failed concentrate sites cell-cell contact, promote morphological...
Type I interferons (IFNs) are pleiotropic cytokines that modulate both innate and adaptive immune responses. They have been used to treat autoimmune disorders, cancers, viral infection demonstrated elicit differential responses within cells, despite sharing a single receptor. The molecular basis for such has remained elusive. To identify the mechanisms underlying type IFN signaling, we whole genome microarrays measure longitudinal transcriptional events human CD4(+) T cells treated with...
Genetic polymorphisms and variable expression of drug receptors, metabolizing enzymes, transporters have been linked to interindividual differences in efficacy toxicity many Food Drug Administration-approved therapeutic agents. In multiple sclerosis, the combination heterogeneity disease pathology significant variation clinical response disease-modifying agents necessitates definition biomarkers that can a priori predict define appropriate regimens. Pharmacogenomic studies will directly...
Background: Whereas cellular immune function depends on energy supply and mitochondrial function, little is known the impact of immunotherapies metabolism. Objective: The objective this paper to assess effects interferon-beta (IFN-β) CD4 + T cells. Methods: Intracellular adenosine triphosphate (iATP) in phytohemagglutinin (PHA)-stimulated cells multiple sclerosis (MS) patients treated with IFN-β controls were analyzed a luciferase-based assay. Mitochondrial-transmembrane potential (ΔΨm)...
Stable dry-state storage of DNA is desirable to minimize required space and reduce electrical shipping costs. purified from various commercially available stabilization matrices has been used successfully in downstream molecular applications (e.g., quantitative polymerase chain reaction [qPCR], microarray, sequence-based genotyping). However, standard conditions still include freezing eluted aqueous buffers or nuclease-free water. Broad implementation dry-state, long-term requires...
Abstract Acute lymphoblastic leukemia (ALL) in children is associated with a distinct neonatal cytokine profile. The basis of this immune phenotype unknown but potentially related to maternal-fetal receptor interactions. We conducted case-control study 226 case child-mother pairs and 404 control evaluate the role interaction between HLA genotypes offspring maternal killer immunoglobulin-like (KIR) etiology childhood ALL, while considering potential mediation by cytokines immune-modulating...
The paper presents a new high level design tool and methodology that are used to accelerate the process. named CSLC starts with description of interfaces, hierarchies connections generating verification infrastructure composed interconnect, hierarchy, test benches, simulation model, vectors documentation. Design specification is based on Chip Specification Language (CSL) brings significant reduction in setup maintenance complex designs. fundamental idea CSL there single source for all...