The mitochondrial pH gradient across the inner-membrane is stabilised by buffering of matrix. A low-molecular mass buffer compound has to be localised in matrix maintain its alkaline value. Taurine found ubiquitously animal cells with concentrations millimolar range and pKa value determined 9.0 (25°C) 8.6 (37°C), respectively. Localisation such a matrix, transforms into biochemical reaction chamber for important matrix-localised enzyme systems. Three acyl-CoA dehydrogenase enzymes, which are...

Different formulations containing Pluronic F127 and polysaccharides (chitosan, sodium alginate, gellan gum, κ-carrageenan) were investigated as potential injectable gels that behave free-flowing liquid with reduced viscosity at low temperatures displayed solid-like properties 37 °C. In addition, ZnO nanoparticles, lysozyme, or curcumin added for testing the antimicrobial of thermal-sensitive gels. Rheological investigations evidenced small changes in transition temperature kinetics gelation...

This paper reports new physical hydrogels obtained by the freezing/thawing method. They include pullulan (PULL) and poly(vinyl alcohol) (PVA) as polymers, bovine serum albumin (BSA) protein, a tripeptide, reduced glutathione (GSH). In addition, sample containing PULL/PVA lysozyme was in similar conditions. SEM analysis evidenced formation of networks with porous structure. The average pore size found to be between 15.7 μm 24.5 μm. All samples exhibited viscoelastic behavior typical networks,...

Currently, ultrashort oligopeptides consisting of fewer than eight amino acids represent a cutting-edge frontier in materials science, particularly the realm hydrogel formation. By employing solid-phase synthesis with Fmoc/tBu approach, novel pentapeptide, FEYNF-NH2, was designed, inspired by previously studied sequence chosen from hen egg-white lysozyme (FESNF-NH2). Qualitative peptide analysis based on reverse-phase high performance liquid chromatography (RP-HPLC), while further...

The fluorenyl methyl oxycarbonyl phenylalanyl-phenylalanine ester (Fmoc-Phe-Phe-Ome) was synthetized using the liquid phase synthesis strategy. This derivative separated by hydrophobic interaction chromatography, its purity analyzed RP-HPLC and it characterized mass spectrometry. extremely peptide conjugate incorporated into aqueous solutions of Pluronic® F127 at low temperatures (below 10 °C). temperature induced sol–gel transition investigated rheological measurements. A delay transition,...

The need for a simple and accurate method protein estimation in alcoholic extracts led to the reexamination of optimum conditions colorimetric assay based on biuret reaction. Sonication time other experimental parameters were optimized after kinetics study extraction either zein or total proteins. Zein purity investigated by (1)H (13)C NMR spectroscopy, SDS-PAGE electrophoresis, UV-visible spectrophotometry (UV-vis). A was proposed, which involves reaction copper ions phosphate powder with...

Peptides have been used as components in biological analysis and fabrication of novel sensors due to several reasons, including well-known synthesis protocols, diverse structures, acting highly selective substrates for enzymes. Bio-conjugation strategies can provide a simple efficient way convert peptide-analyte interaction information into measurable signal, which be further the manufacture new peptide-based biosensors. This paper describes sensitive properties peptide-modified graphene...

In situ-forming gels with self-assembling and self-healing properties are materials of high interest for various biomedical applications, especially drug delivery systems tissue regeneration. The main goal this research was the development an innovative gel carrier based on dynamic inter- intramolecular interactions between amphiphilic polyurethane peptide structures. architecture adapted to achieve desired amphiphilicity self-assembly into aqueous solution facilitate array connections...

In this study, a new strategy was adopted for obtaining polymer/protein hybrid hydrogels with shape stability and tunable mechanical or rheological characteristics by using non-toxic procedures. A chemical network created poly(vinyl alcohol)(PVA)/bovine serum albumin (BSA) mixture in aqueous solution the presence of genipin reduced glutathione (GSH). Then, second physical formed through PVA after applying freezing/thawing cycles. addition, protein macromolecules intermolecular disulfide...

Abstract The extreme toxicity of cyanide, its wide industrial application as well continued illegal use generate research interest in different fields science, imposing multidisciplinary approach to study cyanide poisoning. We show here that the reaction between and ninhydrin can be performed at ambient conditions; however, reagent has freshly prepared oxygen free solvent. Besides, we reading absorbance 485 nm might more suitable reliable than 590 nm, where pH-dependent blue colored...

Numerous hazardous chemicals from various industrial sources enter the environment daily; some of them can be used both as drugs and toxins. Many these compounds, including dinitrophenols, such 2,4-dinitrophenol (2,4-DNP), 4,6-dinitrocresol (DNOC), 2-sec-butyl-4,6-dinitrophenol (dinoseb), are widely pesticides that persist in contaminated soils. They have been found groundwater, causing health environmental hazards, subjects forensic toxicology. Dinitrophenols multiple biological noxious...

Peptides and their related compounds can self-assemble into diverse nanostructures of different shapes sizes in response to various stimuli such as pH, temperature or ionic strength. Here we report the synthesis characterization a lysozyme derived pentapeptide its ability build well-defined fibrillar structures. Lysozyme FESNF peptide fragment was synthesized by solid phase using Fmoc/t-Bu strategy, purified analytical high-performance liquid chromatography (HPLC) molecular weight confirmed...

Complexes of uranyl(VI) (U(VI)) with tridentate ligands derived from salicylaldehyde and aminoacids are reported, bis 2-((2-hydroxybenzylidene)amino)acetic acid U(VI), UO2(SalGly)2 (1); tri aqua 2-((2-hydroxybenzylidene)amino)propanoic U(VI) nitrate, [UO2(SalAla)(H2O)3]NO3 (2); 3-hydroxy-2-((2-hydroxybenzylidene)amino)propanoic [UO2(SalSer)(H2O)3]NO3 (3); 2-((2-hydroxybenzylidene)amino)-3-methylbutanoic [UO2(SalVal)(H2O)3]NO3 (4), 2-((2-hydroxybenzylidene)amino)-4-(methylthio)butanoic...

When the Na,K-ATPase pumps at each turnover two K(+) ions into cytoplasm, this translocation consists of several reaction steps. First, diffuse consecutively from extracellular phase through an access pathway to binding sites where they are coordinated. In next step, enzyme is dephosphorylated and occluded inside membrane domain. The subsequent transition E1 conformation produces a deocclusion cytoplasmic side allows in last steps ion dissociation diffusion aqueous phase. interaction...

Zein is a type of prolamin storage protein that has variety biomedical and industrial applications. Due to the considerable genetic variability polyploidity starting material, as well extraction methods used, characterization composition zein requires combination different analytical processes. Therefore, we combined modern such mass spectrometry (MS), Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), atomic force microscopy (AFM), or Fourier transform infrared...

A significant interest was granted lately to enzymes, which are versatile catalysts characterized by natural origin, with high specificity and selectivity for particular substrates. Additionally, some enzymes involved in the production of high-valuable products, such as antibiotics, while others known their ability transform emerging contaminates, dyes pesticides, simpler molecules a lower environmental impact. Nevertheless, use industrial applications is limited reduced stability extreme...

Abstract Bioinspired peptides are attractive biomolecules which can improve our understanding of self‐assembly processes for rational design new peptide‐based materials. Herein, a amidated peptide FRSAPFIE (FRS), based on sequence present in human collagen, was synthesized, characterized by mass spectrometry and subjected to self‐assembling investigations. The optimal conditions were disclosed dynamic light scattering at 32 °C concentration 0.51 %. In addition, AFM studies revealed...

Cyanide is an extremely severe poison that has been raising concerns and controversies regarding its toxic effects on humans the environment. A short-term human exposure to elevated levels can induce coma or death. Toxic occur at blood cyanide concentrations of 0.05 mg/dL, while death 0.3 mg/dL. Numerous law systems regulate in various environments. To exemplify, maximum contamination limit imposed by United States Environmental Protection Agency drinking water 200 µg/L, European Union...

Mesoporosity of about 7 nm in ETS-10 titanosilicate was generated by postsynthesis treatment with hydrogen peroxide under microwave irradiation, resulting an increased external surface area the materials (55 m 2 /g).The influence porosity and its modification phosphorus atoms (ET(P)S-10) on retention some nanoions (α-emitting radioactive U(VI) Th(IV) ions) biomolecules (the proteins cytochrome c, papain, γ-globulin) investigated.