Introduction: Industrial livestock farming is a possible source of multi-resistant Gram-negative bacteria, including producers extended spectrum beta-lactamases (ESBLs) conferring resistance to 3rd generation cephalosporins. Limited information currently available on the situation ESBL in outside Western Europe. A surveillance study was conducted from January May 2014 four dairy cattle farms different areas Nile delta, Egypt. Materials and Methods: In total, 266 samples were collected 4...

Abstract The emergence of NDM‐producing Escherichia coli has considerably threatened human and animal health worldwide. This study describes for the first time in Egypt, draft genome sequences emerging NDM‐5‐producing E. from humans dogs, investigates genetic relatedness between isolates both sources. Two urine seven environmental clinical samples dogs exhibited resistance to carbapenems harbouring bla NDM were subjected Illumina Miseq whole‐genome sequencing (WGS). Assembly analysis reads...

Background: Adequate training in infectious diseases and antibiotic resistance is crucial for pharmacy students to participate stewardship programs understand microbiology careers. Aim: The study was carried out assess the knowledge self-reported confidence resistance, therapy, antimicrobial (AMS) among final-year undergraduate Sudan. Methods: A cross-sectional conducted three universities using a 57-item online questionnaire between April May 2022. Results: total of 109 (response rate 36%)...

Infected laying hens regularly excrete large amounts of Campylobacter jejuni with their feces, which represent a reservoir infection within the flock and for animals in region. However, knowledge about survival times C. these feces is still scarce. Therefore, orienting laboratory experiments were carried out under controlled conditions to estimate both artificially naturally contaminated hen feces. In 6 different flocks (3 Campylobacter-free 3 Campylobacter-positive flocks), fresh excreta...

Methicillin-resistant Staphylococcus aureus (MRSA) is a major human health problem and recently, domestic animals, in particular pigs poultry are discussed as carriers possible reservoirs of MRSA. Twenty seven S. isolates from five turkey farms (n=18) two broiler (n=9) were obtained by culturing choana skin swabs apparently healthy birds, identified Taqman-based real-time duplex nuc-mecA-PCR characterized spa typing well DNA microarray based assay which covered, amongst others, considerable...

BACKGROUND: Systematic screening for TB using automated chest radiography (ACR) with computer-aided detection software (CAD4TB) has been implemented at scale in Karachi, Pakistan. Despite evidence supporting the use of ACR as a pre-screen prior to Xpert ® MTB/RIF diagnostic testing presumptive patients, there no data published on its mass real-world settings. METHOD: Screening was undertaken mobile digital X-ray vehicles hospital facilities and community camps. Chest X-rays were offered...

The popularity of food produced from animals kept under an organic regimen has increased in recent years. In Germany, turkey meat consumption increased. Despite several studies assessing the susceptibility campylobacters to various antibiotics poultry, no sufficient data exists regarding antimicrobial resistance organic-reared turkeys. This study provides information about antibiotic Campylobacter isolated turkeys reared on farms Germany. Ninety-six strains (41 C. jejuni and 55 coli) were...

A loop-mediated isothermal amplification (LAMP) assay system was established, allowing rplD gene-based simultaneous detection of Campylobacter jejuni and coli in enriched meat products. Additionally, one-step differentiation target species on agar plates enabled by cdtC gene- gyrA duplex LAMP. Both the – LAMP assays amplified sequences all 62 C. 27 strains used for determining inclusivity revealed 100% exclusivity toward 85 tested non-target species. Throughout entire experiments, were...

The need for organic food of animal origin has increased rapidly in recent years. However, effects husbandry on safety have not been rigorously tested especially meat turkey flocks. This study provides the first time an overview prevalence and genetic diversity Campylobacter species (spp.) five farms located different regions Germany, as well potential risk factors bacterial spreading. Thirty cloacal swabs water samples darkling beetles were collected from each flock examined presence by...

The detection of bacterial pathogens from complex sample matrices by PCR requires efficient DNA extraction. In this study, a protocol for extraction and purification swabs, air, water samples using microfluidic chip system was established. optimized includes combination thermal, chemical enzymatic lysis followed chip-based magnetic particles. procedure tested Gram-positive Bacillus thuringiensis Berliner var. kurstaki as model organism anthracis the attenuated live vaccine strain Francisella...

Abstract The objective of the present study was to evaluate potential effect dietary calcium butyrate on growth performance, carcass traits and gut health in Japanese quails. In total, 320 one‐day‐old quails were randomly assigned 4 equal treatments, with 8 replicates 10 quails, for weeks. control treatment fed diet whereas other treatments supplemented at 0.3, 0.5 0.7 g/kg diet. Data concerning performance measurements recorded weekly. addition, eight (one/replicate) from each selected...

Objective: To investigate the prevalence of Bacillus cereus in milk and dairy products along with detection its antibiotic sensitivity.Design: Descriptive study.Samples: One hundred fifty samples market milk, ultra high temperature packs (UHT), condensed Milk powder, Damietta cheese, Kariesh cheese Ras cheese.Procedures: Samples were examined for isolation identification spp.via direct indirect isolation, molecular examination antimicrobial resistance.Further was carried out 46 isolates to...

1. This study investigated the growth performance, caecal microbiota, blood chemistry, splenic cytokines, serum immunoglobulins, carcase, meat quality and oxidative status of broiler chickens fed diets supplemented with Anacardium occidentale leaf powder (AOLP) in comparison antibiotic synthetic antioxidant.2. Three hundred twenty, one-day old Arbor Acre chicks were randomly allotted into four treatment groups consisting eight pen replicates 10 birds per pen. The T0, basal diet only; T1, +...

The aim of the present study was to examine possible effects a different protein supply on presence Campylobacter in excreta and caecal content broiler chickens. Therefore, 220 one-day-old chickens were fed five diets experimental period (day 8 onwards). In control group (CON; n = 3 boxes), soya bean-based industrially produced complete diet containing 5–10% whole wheat coccidiostats fed, whereas remaining groups, four own-made with sources combinations (4 × 2 boxes; bean meal-based SBM;...

Introduction Intravenous thrombolysis with tissue plasminogen activator (tPA) and endovascular therapy (ET) represent the cornerstone of modern stroke management. Despite advancements in these therapies, a subset patients fails to achieve optimal recanalization (TICI 2b‐3), which is associated poor functional outcomes higher mortality rates. As result, there has been growing interest adjunctive pharmacologic interventions that could enhance efficacy ET. The safety tirofiban as an adjunct ET...

Background and Objective: Foot Pad Dermatitis (FPD) is a widespread problem in poultry production constitutes welfare issue.The objective of this study was to test the surplus dietary levels methionine its sources (DL-Met/MHA) on performance health foot pad broilers.Methodology: One-week-old & broilers were divided randomly into 5 groups for 28 days.All diets basically composed wheat (64%) soybean meal (30%).One group fed basic level Met diet (3.5 g kgG 1 diet) without any...

Abstract: A Granulomas can appear in a variety of pathological situations. So, recognizing the granulomatous pattern is essential. because it confined to limited number disorders, some which are life-threatening. There little data on cells that contribute formation granulomas camels. This study identified associated with disorder based histological findings. The intestinal tissues were brought Pathology Department, Faculty Veterinary Medicine, Aswan University, lesions for evaluation. One...

This study was conducted on six breeds of dairy goats (Nubian, cross Nubian and Saidi, Aradi, Shami, Sheraky) in an attempt to explore the in-breeding differences which are reflected milk produced from each breed. The gross components were analyzed yogurt prepared each. viability two strains starters (Lactobacillus bulgaricus Streptococcus thermophilus) used manufacturing measured, as well sensory rheological properties yogurt. results that fat TS percentages higher 2 goat (Nubian Shami)...