A5088120256- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Retinal and Optic Conditions
- Intraocular Surgery and Lenses
- Ocular Diseases and Behçet’s Syndrome
- Ophthalmology and Visual Impairment Studies
- CRISPR and Genetic Engineering
- Cytomegalovirus and herpesvirus research
- Magnetic properties of thin films
- Corneal surgery and disorders
- Semiconductor materials and interfaces
- Inflammatory mediators and NSAID effects
- Psychosomatic Disorders and Their Treatments
- Ocular Oncology and Treatments
- Magnetic Properties and Applications
- Herpesvirus Infections and Treatments
- Immune Cell Function and Interaction
- ZnO doping and properties
- Renal Transplantation Outcomes and Treatments
- Botulinum Toxin and Related Neurological Disorders
- CAR-T cell therapy research
- Parkinson's Disease and Spinal Disorders
- Monoclonal and Polyclonal Antibodies Research
RIKEN Center for Biosystems Dynamics Research
2016-2021
Kobe Kaisei Hospital
2021
University of Tsukuba
2006-2020
Kyoto University
2019
National Hospital Organization Mito Medical Center
2011-2015
Highlights•We established RPE cells from iPSCs in MHC homozygote animals•MHC-mismatching transplantation caused immune attacks of iPS-RPE allografts•MHC-matching appeared to prevent the allografts•T responded allogeneic iPS-RPE, but failed respond RPESummaryThere is an ongoing controversy as whether major histocompatibility complex (MHC) matching a solution for stem cell transplantation. In present study, we retinal pigment epithelial (RPE) induced pluripotent (iPSCs) donors. We observed no...
Allografts of retinal pigment epithelial (RPE) cells have been considered for the treatment ocular diseases. We recently started transplantation induced pluripotent stem cell (iPSC)-derived RPE patients with age-related macular degeneration (autogenic grafts). However, there are at least two problems this approach: (1) high cost, and (2) uselessness acute patients. To resolve these issues, we established from iPSCs in HLA homozygote donors. In vitro, human T directly recognized allogeneic...
ESC- and iPSC-derived retinal transplantation is a promising therapeutic approach for disease with end-stage degeneration, such as retinitis pigmentosa age-related macular degeneration. We previously showed medium- to long-term survival, maturation, light response of transplanted human iPSC-retina in mouse, rat, monkey models Because the use patient hiPSC-derived retina disease-causing gene mutation not appropriate use, allogeneic using tissue/cells differentiated from stocked hESC iPSC line...
Antibody-mediated rejection is characterized by donor-specific antibody produced B cells. However, to our knowledge, cell invasion and in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE could be performed using allografts, we established vivo immune models with induced pluripotent stem (iPSC)-RPE allografts determined whether RPE-specific detected these models. We alloantibodies serum from recipient monkeys that...
Purpose: To determine whether human induced pluripotent stem (iPS) cell–derived retinal pigment epithelial (RPE) cells (iPS-RPE) can suppress natural killer (NK) cell activation. Methods: iPS-RPE were cocultured with peripheral blood mononuclear (PBMCs) or purified NK from healthy donors after stimulation cytokines. confirm expression of cell–specific markers, flow cytometry and quantitative RT-PCR (qRT-PCR) performed. (or PBMCs) assessed for proliferation by Ki-67 cytometry, suppression RPE...
Recently, we successfully transplanted an autograft, or major histocompatibility complex (MHC)-matched allografts, from induced-pluripotent-stem-cell-derived retinal pigment epithelial (iPSC-RPE) cells in patients with age-related macular degeneration. However, there was issue regarding immune rejection after transplantation. In this study, established a preoperational vitro “drug–lymphocytes–grafts reaction (Drug-LGIR)” test to determine the medication for using host immunocompetent...
Abstract In patients with retinitis pigmentosa (RP), color fundus photography and autofluorescence (FAF) have been used to estimate the disease progression. To understand origin diagnostic interpretation of FAF, we performed in vivo imaging FAF together histological analyses retinal degeneration process using RP model mice, rd10 . partly represented accumulation microglia photoreceptor outer segments. Fundus whitening suggested presence apoptotic cells, which spatiotemporally preceded...
Currently, retinal pigment epithelium (RPE) transplantation includes sheet and single-cell transplantation, the latter of which cell death may be highly immunogenic, there are some issues to improved in transplantation. Y-27632 is an inhibitor Rho-associated protein kinase (ROCK), downstream Rho. We herein investigated effect vitro on derived from induced pluripotent stem cells (iPS-RPE cells), also its effects vivo iPS-RPE suspensions. As a result, addition showed suppression apoptosis,...
Purpose: To determine whether human induced pluripotent stem (iPS) cell-derived retinal pigment epithelial (RPE) cells (iPS-RPE) can express complement factors. Methods: confirm expression of factors in iPS-RPE cells, we performed flow cytometry, immunohistochemistry, ELISA, and quantitative RT-PCR for the following: C3, C5, CFB (Factor B), C5b-9 (membrane attack complex [MAC]), CFH H), CFI I), CD46, CD55, CD59, clusterin, vitronectin. We also prepared presence recombinant IFN-γ, TNF-α,...
A tunnel magnetoresistance has been investigated for Fe3Si/CaF2/Fe3Si magnetic junctions (MTJs). We fabricated Fe3Si(20 nm)/CaF2(2 nm)/Fe3Si(15 nm) heterostructures on a CaF2(2 nm)/Si(111) substrate by molecular beam epitaxy (MBE) and processed them into 300×15 mm2-area MTJs using selective wet chemical etching conventional photolithography. The current-voltage (J-V) characteristics the measured at room temperature (RT) were well fitted to Simmons' equation. fitting yields barrier height ϕ =...
A novel anti-mouse CD3ε antibody, Dow2, recognizes mouse without activating T cells and suppresses T-cell activation. The purpose of this study was to determine whether Dow2 can inhibit in uveitis. Experimental autoimmune uveitis (EAU) induced mice by immunization with retinal peptides, followed administration Dow2. Inflammation evaluated color fundus photography, optical coherence tomography, fluorescein angiography, histology. Intraocular from EAU were used examine the effect on...
Purpose: To report occurrence of acute severe inflammation after surgical implantation mycoplasma-infected induced pluripotent stem cell–derived RPE (iPS-RPE) cells into the eyes healthy primates, and determine immunopathological mechanisms inflammation. Methods: Ophthalmic allogeneic transplantation iPS-RPE was performed in subretina major histocompatibility complex (MHC)-matched (two eyes) MHC-mismatched (one eye) cynomolgus monkeys. The clinical course observed using color fundus...
The occurrence of ocular metastasis from lung cancer is uncommon. present study reports the case a 69-year-old female patient with adenocarcinoma who was found to have metastatic lesion in left choroid at time presentation. As mutation epidermal growth factor receptor, treatment gefitinib administered; however, response evaluated as progressive disease. Thereafter, received chemotherapy carboplatin, pemetrexed and bevacizumab. Radiological imaging revealed shrinkage primary choroidal...
In an intraocular inflammatory state, microglia residing in the retina become active and migrate inside retina. this study, we investigated whether cyclooxygenase-1 (COX-1) expressed by retinal microglia/macrophage can be a biomarker for diagnosis of diseases. COX-1 was immunopositive neutrophils, while COX-2 astrocytes neurons inner layer normal The number positive cells per section tissue 14 ± 2.8 (mean standard deviation) mice, which showed significant increase lipopolysaccharide...
Fe3Si/CaF2/Fe3Si magnetic tunnel junctions (MTJs) have been investigated to demonstrate the magnetoresistance effects. We fabricated Fe3Si(20 nm)/CaF2(2 nm)/Fe3Si(15 nm) heterostructures epitaxially on a Si(111) substrate by molecular beam epitaxy. The current-voltage characteristics for MTJs measured at room temperature (RT) were well fitted Simmons' equation. fitting yields barrier height φ=2.5 eV and thickness d=1.26 nm. ratio approximately 0.28% under bias voltage of 20 mV RT.