A5067352783- Pancreatic function and diabetes
- Diabetes and associated disorders
- Single-cell and spatial transcriptomics
- Diabetes Management and Research
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- Nuclear Structure and Function
- Lipid metabolism and biosynthesis
Florida State University
2022-2024
Epidemiology studies demonstrate that women are at a significantly lower risk of developing type 2 diabetes (T2D) compared to men. However, the molecular basis this difference is not well understood. In study, we examined sex differences in genetic programs pancreatic endocrine cells. We combined pancreas perifusion data and single-cell genomic from our laboratory publicly available sets investigate multiple axes human level. systematically female male islet secretion function, gene...
The pancreatic islet is the functional and structural unit of endocrine portion. Islet remodeling occurs in both normal development pathogenesis type 1 (T1D) 2 diabetes (T2D). However, accurately quantifying changes cellular makeup hormone expressions poses significant challenges due to large intra- inter-donor heterogeneity limited scalability traditional methods such as immunostaining. cytometry by time-of-flight (CyTOF) technology enables simultaneous quantification more than 30 protein...
Current approaches to profile the single-cell transcriptomics of human pancreatic endocrine cells almost exclusively rely on freshly isolated islets. However, islets are limited in availability. Furthermore, extensive processing steps during islet isolation and subsequent single cell dissolution might alter gene expressions. In this work, we report development a single-nucleus RNA sequencing (snRNA-seq) approach with targeted enrichment for endocrine-population focused transcriptomic...
Abstract In many cell types, disparate non-centrosomal microtubule-organizing centers (ncMTOCs) replace functional centrosomes and serve the unique needs of types in which they are formed. Drosophila fat body cells (adipocytes), an ncMTOC is organized on nuclear surface. This perinuclear anchored by Msp300, encoded one two Nesprin-encoding genes . Msp300 spectraplakin short stop (shot) co-dependent for localization to envelope generate where recruit microtubule minus-end stabilizer Patronin...