A5100669019- Pancreatic function and diabetes
- Diabetes Management and Research
- Diabetes and associated disorders
- Medical Imaging and Analysis
- Medical Imaging Techniques and Applications
- Scoliosis diagnosis and treatment
- Radiomics and Machine Learning in Medical Imaging
- Metabolism, Diabetes, and Cancer
- Diet, Metabolism, and Disease
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Organ and Tissue Transplantation Research
- Transplantation: Methods and Outcomes
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Advanced X-ray and CT Imaging
- Cannabis and Cannabinoid Research
- Electrohydrodynamics and Fluid Dynamics
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Pediatric Hepatobiliary Diseases and Treatments
- Organ Transplantation Techniques and Outcomes
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Pancreatitis Pathology and Treatment
University of Pennsylvania
2010-2024
Hospital of the University of Pennsylvania
2000-2024
California University of Pennsylvania
2024
Minzu University of China
2023
Institute of Immunology
2023
Philadelphia University
2020
Transplantation of stem cell-derived β (SC-β) cells represents a promising therapy for type 1 diabetes (T1D). However, the delivery, maintenance, and retrieval these remain challenge. Here, we report design safe functional device composed highly porous, durable nanofibrous skin an immunoprotective hydrogel core. The consists electrospun medical-grade thermoplastic silicone-polycarbonate-urethane is soft but tough (~15 megapascal at rupture strain >2). Tuning nanofiber size to less than ~500...
The global diabetes epidemic poses a major challenge. Epigenetic events contribute to the etiology of diabetes; however, lack epigenomic analysis has limited elucidation mechanistic basis for this link. To determine epigenetic architecture human pancreatic islets we mapped genome-wide locations four histone marks: three associated with gene activation—H3K4me1, H3K4me2, and H3K4me3—and one repression, H3K27me3. Interestingly, promoters highly transcribed insulin glucagon genes are occupied...
We sought to compare the efficacy, risks, and costs of whole-organ pancreas transplantation (WOP) with isolated islet (IIT) in treatment patients type I diabetes mellitus.A striking improvement has taken place results IIT regard attaining normoglycemia insulin independence diabetic recipients. Theoretically, this minimally invasive therapy should replace WOP because its risks expense be less. To date, however, no systematic comparisons these 2 options have been reported.We conducted a...
Proteolytic cleavage of proglucagon by prohormone convertase 2 (PC2) is required for islet α cells to generate glucagon. However, the regulatory mechanisms underlying this process remain largely unclear. Here, we report that SEL1L-HRD1 endoplasmic reticulum (ER)-associated degradation (ERAD), a highly conserved protein quality control system responsible clearing misfolded proteins from ER, plays key role in glucagon production regulating turnover nascent proform PC2 enzyme (proPC2). Using...
Type 1 diabetes (T1D) is a chronic condition in which beta cells are destroyed by immune cells. Despite progress immunotherapies that could delay T1D onset, early detection of autoimmunity remains challenging. Here, we evaluate the utility machine learning for prediction using single-cell analysis islets. Using gradient-boosting algorithms, model changes gene expression single from pancreatic tissues and non-diabetic organ donors. We assess if mathematical modeling predict likelihood...
NEUROGENIN3+ (NEUROG3+) cells are considered to be pancreatic endocrine progenitors. Our current knowledge on the molecular program of NEUROG3+ in humans is largely extrapolated from studies mice. We hypothesized that single-cell RNA-seq enables in-depth exploration rare directly humans. aligned four large datasets postnatal human pancreas. integrated analysis revealed 10 epithelial a total 11,174 cells. Noticeably, clustered with mature and epsilon displayed highest frequency NEUROG3...
SPECT nuclear medicine imaging is an effective means of clinically diagnosing bone metastatic diseases. This paper proposes a ResNet improvement model combining Convolutional Block Attention Module (CBAM) and contextual transformer (CoT) attention mechanism to achieve accurate classification images. The improved incorporates the CoTattention into residual module network effectively utilize global information image features. A CBAM module, which combines channel spatial attention, added...
Abstract After the discovery of insulin, a century ago, extensive work has been done to unravel molecular network regulating insulin secretion. Here we performed chemical screen and identified AZD7762, compound that potentiates glucose-stimulated secretion (GSIS) human β cell line, healthy type 2 diabetic (T2D) islets primary cynomolgus macaque islets. In vivo studies in mouse models macaques demonstrated AZD7762 enhances GSIS improves glucose tolerance. Furthermore, genetic manipulation...
Automatic segmentation of bone metastases in low resolution SPECT scan images has an extensive clinical application. In this paper, we propose improved UNet3+ network incorporating attention mechanism to realize automatic metastatic lesions. Firstly, by improving the full-scale deep supervision module reduce semantic gap between low-level and high-level features during feature fusion, which helps obtain more refined results. Secondly, performance model is further adding network. Finally,...
Nonhuman primates are the closest animal models to humans with respect genetics and physiology. Consequently, a critical component of immunogenetics research relies on drawing inferences from cynomolgus macaque inform human trials. Despite conserved organization Major Histocompatibility Complex (MHC) between macaques humans, MHC genotyping is challenging due high rates copy number variants, duplications, rearrangements, particularly at class I loci. Furthermore, limited availability...
Current approaches to profile the single-cell transcriptomics of human pancreatic endocrine cells almost exclusively rely on freshly isolated islets. However, islets are limited in availability. Furthermore, extensive processing steps during islet isolation and subsequent single cell dissolution might alter gene expressions. In this work, we report development a single-nucleus RNA sequencing (snRNA-seq) approach with targeted enrichment for endocrine-population focused transcriptomic...
Abstract Autoimmune destruction of pancreatic β cells results in type 1 diabetes (T1D), with immune infiltrate representing a key feature this process. Studies human T1D immunobiology have predominantly focused on circulating the blood, while mouse models suggest diabetogenic lymphocytes primarily reside pancreas-draining lymph nodes (pLN). A comprehensive study was conducted using pancreas draining lymphatic tissues, including pLN and mesenteric nodes, spleen from non-diabetic control, cell...
Objective In this paper, we introduced a simple and easy way to do islet transplantation under kidney capsule in mouse, the effect evaluation of it’s blood glucose, oral glucose tolerance histological stain after transplantation. Methods BALB/c mice are used experiment.The islets were harvested by reversely perfusion collagenase V into common bile duct purification Histopaque density gradient followed manual picking. After culture, transplanted diabetic BALB/c using gel loading tip....
Abstract Type 1 diabetes (T1D) is a chronic condition in which the insulin-producing beta cells are destroyed by immune cells. Research past few decades characterized involved disease pathogenesis and has led to development of immunotherapies that can delay onset T1D two years. Despite this progress, early detection autoimmunity individuals who will develop remains challenge. Here, we evaluated potential combining single-cell genomics machine learning strategies as prime approach tackle We...
Fuel stimulated hormone release (HR) and the oxygen consumption rates (OCR) of isolated normal pancreatic islets are positively correlated illustrating that cellular energy state is a critical determinant stimulus-secretion coupling. We investigated relationship between OCR in from donors with T1DM T2DM, using unique measurement technology optimized perifusion protocols. HR were quantified 20 human donor islet preparations obtained Human Islet Resource Center at University Pennsylvania...
Liu, Chengyang1; Deng, Shaoping1; Wenying1; Kenyon, Norma2; Ricordi, Camillo2; Brayman, Kenneth L.1 Author Information
The purpose of this study is to develop low-cost real-time digital fringe projection measurement detect the three-dimensional shape back in patients with scoliosis. system consists a projector and camera. seven patterns are used illuminate patient’s while images captured by height calculated using seven-step phase-shifting calculation path-independent phase unwrapping. topography an accuracy 1 mm can help clinician estimate spinal deformity at baseline monitor variations over time. results...