A5061965053- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
- Genetic and Kidney Cyst Diseases
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Heme Oxygenase-1 and Carbon Monoxide
- DNA Repair Mechanisms
- Medical Coding and Health Information
- Methane Hydrates and Related Phenomena
- Glycosylation and Glycoproteins Research
- Barrier Structure and Function Studies
- Retinal Diseases and Treatments
- Insurance, Mortality, Demography, Risk Management
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Cell Adhesion Molecules Research
- Microbial Community Ecology and Physiology
- Data Analysis and Archiving
- Peptidase Inhibition and Analysis
- Enzyme Structure and Function
McGill University
2019-2023
Wayne State University
1980
Microtubules are cytoskeletal structures involved in stability, transport and organization the cell. The building blocks, α- β-tubulin heterodimers, form protofilaments that associate laterally into hollow microtubule. Microtubule also exists as highly stable doublet microtubules cilia where stability is needed for ciliary beating function. microtubule maintains its through interactions at inner outer junctions A- B-tubules meet. Here, using cryo-electron microscopy, bioinformatics mass...
Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed complex network inside the lumen both tubules composed microtubule-inner proteins (MIPs). However, functions most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses Tetrahymena thermophila...
Cilia, the hair-like protrusions that beat at high frequencies to propel a cell or move fluid around are composed of radially bundled doublet microtubules. In this study, we present near-atomic resolution map Tetrahymena microtubule by cryoelectron microscopy. The demonstrates network inner proteins weaves into tubulin lattice and forms an sheath. From mass spectrometry data de novo modeling, identified Rib43a as filamentous in protofilament ribbon region. Rib43a–tubulin interaction leads...
Abstract Cryo-electron microscopy (cryo-EM) maps usually show heterogeneous distributions of B -factors and electron density occupancies are typically -factor sharpened to improve their contrast interpretability at high-resolutions. However, ‘over-sharpening’ due the application a single global can distort processed causing connected densities appear broken disconnected. This issue limits cryo-EM maps, i.e. ab initio modelling. In this work, we propose 1) approaches enhance high-resolution...
Abstract Cilia, the hair-like protrusions that beat at high frequencies to propel a cell or move fluid around cell, are composed of radially bundled doublet microtubules. The microtubule is 13-protofilament A-tubule, partial 10-protofilament B-tubule and inner proteins (MIPs) inside tubulin lattice. In this study, we present near-atomic resolution map Tetrahymena demonstrates network weaving into lattice, forming an sheath proteins. addition, also obtain lattice structure with missing MIPs...
Abstract Cryo-electron microscopy (cryo-EM) maps usually show heterogeneous distributions of B-factors and electron density occupancies are typically B-factor sharpened to improve their contrast interpretability at high-resolutions. However, ‘over-sharpening’ due the application a single global can distort processed causing connected densities appear broken disconnected. This issue limits cryo-EM maps, i.e. ab initio modelling. In this work, we propose 1) approaches enhance high-resolution...
Abstract In human, the Parkin Co-Regulated Gene (PACRG) shares a bidirectional promoter with Parkin, gene involved in Parkinson’s disease, mitochondrial quality control and inflammation. The PACRG protein is essential to formation of inner junction between doublet microtubules axoneme, structure found flagella cilia. interacts tubulin as well meiosis expressed 1 (MEIG1) protein, which for spermiogenesis mice. However, 3D human unknown. Here, we report crystal C-terminal domain complex MEIG1...
Abstract Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed complex network inside the lumen both tubules composed microtubule-inner proteins (MIPs). However, functions most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses Tetrahymena...
Abstract Microtubules are cytoskeletal structures involved in structural support, microtubule-based transport and the organization of organelles cells. The building blocks microtubule, α- β-tubulin heterodimers, polymerize into protofilaments, that associate laterally to form hollow microtubule. There exists a specific type microtubule cilia, termed doublet microtubules, where high stability is required for ciliary beating function. consisting complete A-tubule partial B-tubule maintains its...
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Cryo-electron microscopy (cryo-EM) has become a powerful technique for protein structure determination. However, there are still important issues to be addressed, such as, robust sharpening or local B-factors estimations. To address these issues, we present here new methods improve the analysis and interpretability of cryo-EM maps.