A5044208895- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Bacterial Genetics and Biotechnology
- Micro and Nano Robotics
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
- Genetics, Aging, and Longevity in Model Organisms
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Cancer Treatment and Pharmacology
- Genetics, Bioinformatics, and Biomedical Research
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Genetic and Kidney Cyst Diseases
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Machine Learning in Bioinformatics
- Bacteriophages and microbial interactions
- Biofield Effects and Biophysics
MRC Laboratory of Molecular Biology
2022-2025
Medical Research Council
2022-2024
University of Oxford
2023
University of California, San Francisco
2023
McGill University
2013-2021
ESCRT-III proteins assemble into composite polymers that undergo stepwise changes in composition and structure to deform membranes across the tree of life. Here, using a phylogenetic analysis, we demonstrate two endosomal sorting complex required for transport III (ESCRT-III) present eukaryote's closest Asgard archaeal relatives are evolutionarily related B- A-type eukaryotic paralogs initiate execute membrane remodeling, respectively. We show ESCRT-IIIB assembles parallel arrays on planar...
A compound that binds to tubulin in an unusual way has superior antitumor efficacy and safety a distinctive impact on microtubule curvature dynamics.
Every protein in our cells has evolved to fold into a specific structure perform its functions. However, determining these structures experimentally is often challenging, some cases taking years. Recently, machine-learning algorithms have been designed predict protein’s directly from amino acid sequence minutes hours. Since the release of first algorithms, AlphaFold and RoseTTAFold, several more developed. These complemented by tools that leverage outputs give structural context biochemical...
The diverse roles of the dynein motor in shaping microtubule networks and cargo transport complicate vivo analysis its functions significantly. To address this issue, we have generated a series missense mutations Drosophila Dynein heavy chain. We show that associated with human neurological disease cause range defects, including impaired trafficking neurons. also describe novel microtubule-binding domain mutation specifically blocks metaphase-anaphase transition during mitosis embryo. This...
Summary ESCRT-III proteins assemble into composite polymers that undergo stepwise changes in composition and structure to deform membranes across the tree of life. Here, using a phylogenetic analysis we demonstrate two present our closest archaeal relatives are evolutionarily related B-type A-type eukaryotic paralogues, which initiate execute membrane remodelling, respectively. This deep homology is reflected function as demonstrated by fact ESCRT-IIIB assembles parallel arrays on planar...
Abstract Cytoplasmic dynein is a microtubule motor that activated by its cofactor dynactin and coiled-coil cargo adaptor. There currently limited structural information on how the resulting complex interacts with microtubules adaptors are recruited. Here, we develop cryo-EM processing pipeline to solve high-resolution structure of dynein-dynactin adaptor BICDR1 bound microtubules. This reveals asymmetric interactions between neighbouring domains it relates their motile behaviour. We find...
Abstract In human, the Parkin Co-Regulated Gene (PACRG) shares a bidirectional promoter with Parkin, gene involved in Parkinson’s disease, mitochondrial quality control and inflammation. The PACRG protein is essential to formation of inner junction between doublet microtubules axoneme, structure found flagella cilia. interacts tubulin as well meiosis expressed 1 (MEIG1) protein, which for spermiogenesis mice. However, 3D human unknown. Here, we report crystal C-terminal domain complex MEIG1...
ABSTRACT The cytoplasmic dynein-1 (dynein) motor organizes cells by shaping microtubule networks and moving a large variety of cargoes along them. However, dynein’s diverse roles complicate in vivo studies its functions significantly. To address this issue, we have used gene editing to generate series missense mutations Drosophila Dynein heavy chain (Dhc). We find that associated with human neurological disease cause range defects larval adult flies, including impaired cargo trafficking...
ABSTRACT Andrew Carter studied biochemistry at the University of Oxford, where he discovered his love for structural biology, which prompted him to join Venki Ramakrishnan's lab MRC Laboratory Molecular Biology (LMB) PhD. Here, used X-ray crystallography study ribosome and its interactions with antibiotics. He then spent a year as postdoc Clare College, Cambridge, before moving Ron Vale's California, San Francisco (UCSF), USA, first began work on dyneins. has since devoted research career...