A5021416104- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sexual Differentiation and Disorders
- Animal Genetics and Reproduction
- RNA Research and Splicing
- Vaccine Coverage and Hesitancy
- SARS-CoV-2 and COVID-19 Research
- Poxvirus research and outbreaks
- Viral Infections and Outbreaks Research
- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Virology and Viral Diseases
- Neurogenesis and neuroplasticity mechanisms
- Bacillus and Francisella bacterial research
- Axon Guidance and Neuronal Signaling
- Radiative Heat Transfer Studies
- Intestinal Malrotation and Obstruction Disorders
- Hedgehog Signaling Pathway Studies
- Chromosomal and Genetic Variations
- Nerve injury and regeneration
- Public Health Policies and Education
- Circular RNAs in diseases
- Tissue Engineering and Regenerative Medicine
- Nuclear Structure and Function
- Additive Manufacturing and 3D Printing Technologies
- Topology Optimization in Engineering
Coalition for Epidemic Preparedness Innovations
2021-2025
Carleton University
2019
King's College London
2000-2001
Guy's Hospital
1996-1998
The Francis Crick Institute
1995-1996
Medical Research Council
1993-1995
National Institute for Health Research
1995
Laboratoire de Biologie Moléculaire des Eucaryotes
1993
National Institute for Medical Research
1993
In the mouse, Sry is expressed by germ cells in adult testis and somatic genital ridge. Transcripts former exist as circular RNA molecules of 1.23 kb, which are unlikely to be efficiently translated. We have used RNase protection map extent less abundant transcript developing gonad. demonstrate that it a linear mRNA derived from single exon. This begins unique region 5' protein coding extends several kilobases into 3' arm large inverted repeat bounds genomic locus. Knowledge this transcript,...
ABSTRACT The Sox gene family consists of a large number embryonically expressed genes related via the possession 79-amino-acid DNA-binding domain known as HMG box. Partial clones for first three (a1-a3) were isolated by homology to box testis-determining Sry and are now termed Sox-1, Sox-2 Sox-3. Sox-3 is highly conserved amongst mammalian species located on X chromosome. This has led proposal that evolved from We present cloning sequencing mouse show most closely Sry. also confirm...
ABSTRACT Cells of the cranial paraxial mesoderm give rise to parts skull and muscles head. Some cells migrate from locations close hindbrain into branchial arches where they undergo muscle differentiation. We have characterised these migratory pathways in chick embryos either by DiI-labelling before migration or grafting quail orthotopically. These experiments demonstrate that depending on their initial rostrocaudal position, fill core specific arches. A survey expression myogenic genes...
ABSTRACT During development, cranial motor neurons extend their axons along distinct pathways into the periphery. For example, branchiomotor dorsally to leave hindbrain via large dorsal exit points. They then grow in association with sensory ganglia, targets, muscles of branchial arches. We have investigated possibility that pathway tissues might secrete diffusible chemorepellents or chemoattractants guide axons, using co-cultures collagen gels. found explants neural tube roof plate...
The process of sex determination, by which is meant the decision as to whether an embryo develops a male or female, considered paradigm how gene action can influence developmental fate. In mammals dependent on testis determining present Y chromosome, now known be Sry. Sry expressed for only brief period in mouse and must act initiate rather than maintain pathway activity required differentiation. It probably acts within cells supporting cell lineage direct their differentiation into Sertoli...
During an investigation of the regulatory potential a region 5' mouse anti-müllerian hormone (Amh) gene, we identified homology with known cDNA sequence human spliceosome gene (SAP62). In mouse, Sap62 termination codon (TGA) is just 434 bp Amh start translation (ATG); in equivalent distance 789 bp. RNase protection analysis shows majority transcripts use uncommon polyadenylation signal (ATTAAA) lying intragenic region, 87 3' TGA. This also that transcribed all tissues examined, whereas...
Benefit-risk assessment (BRA) is critical for decision-making throughout the vaccine life cycle. It requires scientific of evidence to make an informed judgment on whether has a favourable benefit-risk profile i.e. benefits outweigh its risks use in intended indication. The must also consider data gaps and uncertainties, using sensitivity analyses show impact these uncertainties assessment. BRA field advanced considerably over past years, including structured frameworks, quantitative models...
We have entered an age of calamity, where the concurrence disruptive forces, including infectious diseases, will continue in ever accelerating fashion. To better prepare for dealing with "polycrises," health regulatory paradigm should to evolve, smart and agile regulation as a key enabler proactively guiding advancement application science. How regulators support innovation be critical prevent another cycle panic neglect. The Centre Regulatory Excellence (CoRE) launched annual lecture series...
Abstract Mpox outbreaks in Central Africa have been declared a public health emergency of international concern by the World Health Organization. Fortunately, real-world effectiveness studies MVA-BN vaccine indicate that it has an 74% after one dose, and 82% two doses against mpox. However, given very limited supply vaccines Africa, there remain questions around optimal deployment doses. In this study, we consider whether more mpox cases might be averted following traditional two-dose...
A case is described of a duplicated lower intestinal tract in broiler chicken. The ileum branched to form two tracts. the malformed branch was swollen and attached paired ceca that were grossly enlarged, rectum blind sac, disconnected from exterior bird.
The research work presented in this paper is developed by the Blended Wing Body (BWB) Unmanned Aerial Vehicle (UAV) team, a fourth-year capstone project at Carleton University Department of Mechanical and Aerospace Engineering. Here, clean sheet UAV with BWB configuration designed optimized using Multiscale Design Optimization (MSDO) approach employing lattice materials taking into consideration design for additive manufacturing constraints. BWB-UAV being mission profile surveillance...