A5014215458- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Smart Materials for Construction
- Neuroscience and Neuropharmacology Research
- Nerve injury and regeneration
- Photoacoustic and Ultrasonic Imaging
- Optical Coherence Tomography Applications
- Axon Guidance and Neuronal Signaling
- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Climate change and permafrost
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Cellular Mechanics and Interactions
- Congenital heart defects research
- Advanced Electron Microscopy Techniques and Applications
- Animal Genetics and Reproduction
- Asphalt Pavement Performance Evaluation
- Single-cell and spatial transcriptomics
- Advanced Biosensing Techniques and Applications
- Signaling Pathways in Disease
- Adenosine and Purinergic Signaling
- Thermography and Photoacoustic Techniques
Institut de la Vision
2014-2024
Inserm
2014-2024
Sorbonne Université
2014-2024
Centre National de la Recherche Scientifique
2014-2024
Université Paris Cité
2008-2011
Cold Spring Harbor Laboratory
2011
Harvard University
2007
Institut de Biologie du Développement Marseille
1997-2004
Centre d'Études et de Recherche en Thermique, Environnement et Systèmes
2004
Paris-Est Sup
2004
The development of a patterned vasculature is essential for normal organogenesis. We found that signaling by semaphorin 3E (Sema3E) and its receptor plexin-D1 controls endothelial cell positioning the patterning developing in mouse. Sema3E highly expressed somites, where it acts as repulsive cue plexin-D1–expressing cells adjacent intersomitic vessels. Sema3E–plexin-D1 did not require neuropilins, which were previously presumed to be obligate Sema3 coreceptors. Moreover, genetic ablation or...
Abstract Astrocytes play essential roles in the neural tissue where they form a continuous network, while displaying important local heterogeneity. Here, we performed multiclonal lineage tracing using combinatorial genetic markers together with new large volume color imaging approach to study astrocyte development mouse cortex. We show that cortical clones intermix their neighbors and display extensive variability terms of spatial organization, number subtypes cells generated. Clones develop...
Multiphoton microscopy combined with genetically encoded fluorescent indicators is a central tool in biology. Three-photon (3P) excitation the short-wavelength infrared (SWIR) water transparency bands at 1.3 and 1.7 µm opens up new opportunities for deep-tissue imaging. However, novel strategies are needed to enable in-depth multicolor fluorescence imaging fully develop such an approach. Here, we report on multiband SWIR source that simultaneously emits ultrashort pulses has characteristics...
Adult neural stem cells and multiciliated ependymal are glial essential for neurological functions. Together, they make up the adult neurogenic niche. Using both high-throughput clonal analysis single-cell resolution of progenitor division patterns fate, we show that these two components niche lineally related: sister to cells, whereas most arise from terminal symmetric divisions lineage. Unexpectedly, found antagonist regulators DNA replication, GemC1 Geminin, can tune proportion cells. Our...
Abstract Large-scale microscopy approaches are transforming brain imaging, but currently lack efficient multicolor contrast modalities. We introduce chromatic multiphoton serial (ChroMS) microscopy, a method integrating one‐shot excitation through wavelength mixing and block-face image acquisition. This approach provides organ-scale micrometric imaging of spectrally distinct fluorescent proteins label-free nonlinear signals with constant micrometer-scale resolution sub-micron channel...
ABSTRACT Muscle-derived factors are known to be important for the survival of developing spinal motoneurons, but molecules involved have not been characterized. Hepatocyte growth factor/scatter factor (HGF/SF) plays an role in muscle development and motoneuron axon outgrowth. We show that HGF/SF has potent neurotrophic activity (EC50=2 pM) a subpopulation (40%) purified embryonic rat motoneurons. Moreover, is essential component muscle-derived support since blocking antibodies specifically...
Hox genes are instrumental in assigning segmental identity the developing hindbrain. Auto-, cross- and para-regulatory interactions help establish maintain their expression. To understand to what extent such regulatory shape neuronal patterning hindbrain, we analysed neurogenesis, differentiation motoneuron migration Hoxa1, Hoxb1 Hoxb2 mutant mice. This comparison revealed that neurogenesis of specific subpopulations r4 was impaired a similar fashion all three mutants, but with different...
ABSTRACT During development, cranial motor neurons extend their axons along distinct pathways into the periphery. For example, branchiomotor dorsally to leave hindbrain via large dorsal exit points. They then grow in association with sensory ganglia, targets, muscles of branchial arches. We have investigated possibility that pathway tissues might secrete diffusible chemorepellents or chemoattractants guide axons, using co-cultures collagen gels. found explants neural tube roof plate...
This protocol describes how to use the Brainbow strategy label neurons in many different hues. The system uses a random Cre/lox recombination create varied combinations of red, blue, and green fluorescent proteins each cell. differences color allow users follow multiple cells, regardless closely they are positioned. imaging zebrafish provides examples as guide trace axonal processes. We trigeminal sensory ganglion an example discuss potential modifications for general this technique.
The spectral window lying between 1.6 and 1.7 μm is interesting for in-depth multiphoton microscopy of intact tissues due to reduced scattering absorption in this wavelength range. However, wide adoption excitation range will rely on the availability robust cost-effective high peak power pulsed lasers operating at these wavelengths. In communication, we report a monolithically integrated repetition rate (50 MHz) all-fiber femtosecond laser based soliton self-frequency shift providing 9 nJ,...
Abstract Astrocytes participate in information processing by releasing neuroactive substances termed gliotransmitters, including ATP. Individual astrocytes come into contact with thousands of synapses their ramified structure, but the spatiotemporal dynamics ATP gliotransmission remains unclear, especially physiological brain tissue. Using a genetically encoded fluorescent sensor, GRAB ATP1.0 , we discovered that extracellular increased locally and transiently absence stimuli neuron–glia...
Glial cell-line derived neurotrophic factor (GDNF) and its relative neurturin (NTN) are potent trophic factors for motoneurons. They exert their biological effects by activating the RET tyrosine kinase in presence of a glycosyl-phosphatidylinositol-linked co-receptor, either GFRα1 or GFRα2. By whole-mount situ hybridization on embryonic mouse spinal cord, we demonstrate that whereas Ret is expressed nearly all motoneurons, Gfra 1 2 exhibit complex distinct patterns expression. Most...
INTRODUCTION Visualizing the precise morphology of closely juxtaposed cells and their interactions can be highly informative, particularly when studying complex organization neuronal glial networks in nervous system. To this end, one use optical approaches to image-distinct markers that are differentially distributed among interest, such as fluorescent proteins various colors (XFPs). The Brainbow strategies Cre/ lox recombination stochastically express two four XFPs a cellular population...
Abstract In the spinal cord, motor neurons (MNs) with similar muscle targets and sensory inputs are grouped together into pools. To date, relatively little is known about molecular mechanisms that control establishment of pool‐specific circuitry. Semaphorins, a large family secreted cell surface proteins, important mediators developmental processes such as axon guidance migration. Here, we used mRNA in situ hybridization to study expression patterns semaphorins their receptors, neuropilins...
Infrared emissivity is a necessary parameter for computing models to predict road surface status and pavement temperature irrespective of the weather situation. In this work new experimental device based on indirect method was developed measurement emissivities. The exposed modulated isotropic infrared radiation. intensity reflected by sample in given direction measured detector operating spectral range 1–40 µm. This large allows total hemispheric defined case as emissivity. effect...
Oligodendrocytes are the myelinating cells of central nervous system. Multiple markers available to analyze populations oligodendroglial and their precursors during development in pathological conditions. However, behavior oligodendrocytes remains poorly characterized vivo , especially at level individual cells. Studying this aspect has been impaired so far by lack suitable methods for visualizing single oligodendrocytes, processes, interactions myelination. Here, we have used multicolor...