A5038590583- Angiogenesis and VEGF in Cancer
- Nonmelanoma Skin Cancer Studies
- Congenital heart defects research
- Skin Protection and Aging
- Cutaneous Melanoma Detection and Management
- Wound Healing and Treatments
- Cancer and Skin Lesions
- Contact Dermatitis and Allergies
- Mesenchymal stem cell research
- Acute Ischemic Stroke Management
- Colorectal Cancer Treatments and Studies
- Single-cell and spatial transcriptomics
- Atherosclerosis and Cardiovascular Diseases
- Dermatologic Treatments and Research
- Cancer, Hypoxia, and Metabolism
- Zebrafish Biomedical Research Applications
- Lipoproteins and Cardiovascular Health
- Skin and Cellular Biology Research
- Estrogen and related hormone effects
- Hedgehog Signaling Pathway Studies
- Cancer Treatment and Pharmacology
- Hair Growth and Disorders
- Ion Transport and Channel Regulation
- Cell Adhesion Molecules Research
- Growth Hormone and Insulin-like Growth Factors
The University of Queensland
2016-2024
Chinese University of Hong Kong
1990-2024
Tseung Kwan O Hospital
2024
Oslo University Hospital
2023
Translational Research Institute
2016-2019
Royal Brisbane and Women's Hospital
2017
National University of Malaysia
2013
Imperial College London
2012
Baylor College of Medicine
1994
During adult life, blood vessel formation is thought to occur via angiogenic processes involving branching from existing vessels. An alternate proposal suggests that neovessels form endothelial progenitors able assemble the intimal layers. We here aimed define vessel-resident in vivo a variety of tissues physiological and pathological situations such as normal aorta, lungs, wound healing, tumors, placenta, well.Based on protein expression levels common markers using flow cytometry, 3...
The cellular and molecular profiles that govern the endothelial heterogeneity of circulatory system have yet to be elucidated. Using a data-driven approach study compartment via single-cell RNA sequencing, we characterized cell subpopulations within assigned them defined hierarchy. We show two transcriptionally distinct populations exist aorta and, using independent trajectory analysis methods, confirm they represent transitioning cells rather than discrete types. Gene co-expression revealed...
We analyzed the serum concentrations of lipids and lipoproteins prevalence other risk factors in a case-control study 304 consecutive Chinese patients with acute stroke (classified as cerebral infarction, lacunar or intracerebral hemorrhage) age- sex-matched controls. For all strokes we identified following factors: history ischemic heart disease, diabetes mellitus, hypertension; presence atrial fibrillation left ventricular hypertrophy; glycosylated hemoglobin A1 concentration greater than...
Abstract Endothelial to mesenchymal transition (EndMT) is a leading cause of fibrosis and disease, however its mechanism has yet be elucidated. The endothelium possesses profound regenerative capacity adapt reorganize that attributed population vessel-resident endovascular progenitors (EVP) governing an endothelial hierarchy. Here, using fate analysis, we show two transcription factors SOX9 RBPJ specifically affect the murine EVP numbers regulate lineage specification. Conditional knock-out...
Tumor vascularization is a hallmark of cancer central to disease progression and metastasis. Current anti-angiogenic therapies have limited success prompting the need better understand cellular origin tumor vessels. Using fate-mapping analysis endothelial cell populations in melanoma, we report very early infiltration endovascular progenitors (EVP) growing tumors. These cells harbored self-renewal reactivated expression SOX18 transcription factor, initiating vasculogenic process as single...
Extracellular vesicles (EVs) secreted by umbilical cord-derived mesenchymal stem cells (UC-MSCs) hold significant promise as therapeutic agents in regenerative medicine. This study investigates the effects of UC-MSC-derived EVs on dermal fibroblast function, and their potential wound healing applications. were characterized nanoparticle tracking analysis transmission electron microscopy, revealing a mean size 118.6 nm, consistent with exosomal properties. Dermal fibroblasts treated varying...
The Shatin Stroke Registry is a prospective study of all patients admitted with acute stroke to general hospital in Hong Kong where the population predominantly Chinese. Each patient was examined by neurologist and 95.5% had brain CT. Of 777 included study, 44.0% cortical/subcortical infarct, 18.5% supratentorial lacunar 24.2% intracerebral hemorrhage, 5.8% brainstem/cerebellar 2.9% 4.5% an uncertain diagnosis. overall 30-day case fatality rate 25.4%. Comparison five registries from West...
Both humans and mice lacking functional growth hormone (GH) receptors are known to be resistant cancer. Further, autocrine GH has been reported act as a cancer promoter. Here we present the first example of variant receptor (GHR) associated with promotion, in this case lung We show that GHRP495T located intracellular domain is able prolong signal vitro using stably expressing mouse pro-B-cell human cell lines. This relevant because secretion pulsatile, extending duration makes it resemble...
The prospect of using endothelial progenitors is currently hampered by their low engraftment upon transplantation. We report that mesenchymal stem/stromal cells (MSCs), independent source and age, improve the colony forming (ECFCs). MSC coculture altered ECFC appearance to an elongated morphology with reduced proliferation. primed via contact had self-renewal potential, but improved capacity form tube structures in vitro vivo Primed ECFCs displayed major differences transcriptome compared...
Abstract Since the discovery of endothelial colony forming cells (ECFC), there has been significant interest in their therapeutic potential to treat vascular injuries. ECFC cultures display heterogeneity and a hierarchy among able give rise high proliferative versus low colonies. Here we aimed define molecularly this vitro hierarchy. Based on flow cytometry, CD34 expression levels distinguished two populations. Only + had capacity reproduce (HPP) colonies replating, whereas CD34− ECFCs...
The main carcinogen for keratinocyte skin cancers (KCs) such as basal and squamous cell carcinomas is ultraviolet (UV) radiation. There growing evidence that accumulation of mutations clonal expansion play a key role in KC development. relationship between UV exposure, epidermal mutation load, KCs remains unclear. Here, we examined the load both murine (n = 23) human 37) samples. Epidermal accumulated dose-dependent manner, this correlated with burden. ablation (either mechanical or laser...
We studied serum lipid profiles in 171 patients less than or equal to 48 hours after the onset of acute stroke and 3 months later. The 83 suffering cerebral infarction had significantly higher concentrations total cholesterol, low density lipoprotein-cholesterol, apolipoprotein B lower triglycerides lipoprotein (a) ictus 53 lacunar were similar on both occasions, only significant differences being cholesterol lipoprotein-cholesterol ictus. No changes observed among 35 hemorrhage apart from a...
Background: The transition to dolutegravir-containing antiretroviral therapy (ART) in low- and middle-income countries (LMICs) was complicated by an initial safety signal May 2018 suggesting that exposure dolutegravir at conception possibly associated with infant neural tube defects. On the basis of additional evidence, July 2019, World Health Organization recommended for all adults adolescents living HIV. Objective: To describe uptake disparities sex age group LMICs. Design: Observational...
Regenerative capabilities of the endothelium rely on vessel-resident progenitors termed endothelial colony forming cells (ECFCs). This study aimed to investigate if these are impacted by conditions (i.e., obesity or atherosclerosis) characterized increased serum levels oxidized low-density lipoprotein (oxLDL), a known inducer Endothelial-to-Mesenchymal Transition (EndMT). Our investigation focused understanding effects EndMT self-renewal and associated molecular alterations. In presence...
SOX family proteins SOX2 and SOX18 have been reported as being essential in determining hair follicle type; however, the role they play during development remains unclear. Here, we demonstrate that Sox18 regulates normal differentiation of dermal papilla all types. In guard (primary) condensate (DC) cells, identified transient addition to expression at E14.5, which allowed fate tracing primary DC cells until birth. Similarly, was detected secondary hairs E16.5 tertiary E18.5....
Apical ATP, UTP and UDP evoked transient increases in short circuit current (I SC , a direct measure of transepithelial ion transport) confluent Caco‐2 cells grown on permeable supports. These responses were mediated by population at least three pharmacologically distinct receptors. Experiments using glass coverslips showed that ATP consistently increased intracellular free calcium ([Ca 2+ ] i ) whilst sensitivity to was variable. Cross desensitization experiments suggested the common...
To better understand the influence of ultraviolet (UV) irradiation on initial steps skin carcinogenesis, we examine patches labeled keratinocytes as a proxy for clones in interfollicular epidermis (IFE) and measure their size variation upon UVB irradiation. Multicolor lineage tracing reveals that chronically irradiated skin, near hair follicles (HFs) increase size, whereas those far from do not change. This is explained by proliferation basal epidermal cells within 60 μm HF openings. Upon...
Introduction: Targeted routine antenatal prophylaxis with anti-D immunoglobulin (Ig) only to RhD-negative pregnant women who carry RhD-positive fetuses (determined by fetal RHD genotyping) has reduced D-alloimmunization significantly when administered in addition postnatal prophylaxis. Achieving high analysis sensitivity and few false-negative results will make RhD typing of the newborn redundant. Postnatal can then be given based on result genotyping. Terminating newborns cord blood...