A5045768319- Hemoglobinopathies and Related Disorders
- Neurofibromatosis and Schwannoma Cases
- Neuroblastoma Research and Treatments
- Blood groups and transfusion
- Virus-based gene therapy research
- Blood donation and transfusion practices
- RNA Interference and Gene Delivery
- HIV/AIDS Research and Interventions
- Neonatal Health and Biochemistry
- Hematological disorders and diagnostics
- Iron Metabolism and Disorders
- Cancer Research and Treatments
- COVID-19 Clinical Research Studies
- Blood disorders and treatments
- Hereditary Neurological Disorders
- CRISPR and Genetic Engineering
- Erythrocyte Function and Pathophysiology
- Malaria Research and Control
- Ion Transport and Channel Regulation
- Digital Imaging for Blood Diseases
- Complement system in diseases
- Clinical Laboratory Practices and Quality Control
- Fibroblast Growth Factor Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Cancer Mechanisms and Therapy
Massachusetts General Hospital
2015-2023
Harvard University
2019-2023
Bayero University Kano
2019
Aminu Kano Teaching Hospital
2019
Creative Commons
2019
Royal Holloway University of London
2011-2017
Beni-Suef University
2017
University of Maiduguri
2000-2007
Ninewells Hospital
2000
University of Dundee
2000
Several diseases and injuries of the central nervous system could potentially be treated by delivery an enzyme, which might most effectively achieved gene therapy. In particular, bacterial enzyme chondroitinase ABC is beneficial in animal models spinal cord injury. We have adapted so that it can direct secretion active from mammalian cells, inserted into lentiviral vectors. When injected adult rat brain, these vectors lead to extensive chondroitinase, both locally long-distance axon...
Schwannomas are slow-growing benign neoplasms that develop throughout the body causing pain, sensory/motor dysfunction, and death. Because bacterial immunotherapy has been used in treatment of some malignant neoplasms, we evaluated attenuated Salmonella typhimurium strains as immunotherapies for murine schwannomas. Several were tested, including VNP20009, a highly strain was previously shown to be safe human subjects with advanced VNP20009 mutant altered motility other properties included...
Nonintegrating gene delivery vectors have an improved safety profile compared with integrating vectors, but transgene retention is problematic as nonreplicating episomes are progressively and rapidly diluted out through cell division. We developed integration-deficient lentiviral vector (IDLV) system generating mitotically stable capable of long-term expression. found that a transient cycle arrest at the time transduction IDLVs resulted in 13-45% Chinese hamster ovary (CHO) cells expressing...
Abstract Background We evaluated apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) as schwannoma tumor suppressor and explored its utilization in gene therapy strategy that may be translated to clinical use. Methods ASC expression mRNA level were assessed human by immunohistochemistry quantitative PCR, respectively. Methylation- specific PCR was used assess promoter methylation. The effect of overexpression cells through ATP-based viability, lactate...
In a retrospective analysis, the quantities, patterns and adequacy of blood donations made, between 1984 2006, at University Maiduguri Teaching Hospital in north–eastern Nigeria were explored related to safety study area. The types donor reviewed annual increments number made estimated compared with numbers in-patients managed hospital. mean increment (4%) fell well below in-patient (11%). received hospital into four types: voluntary, family-replacement, commercial pre-deposit autologous...
BACKGROUND AND PURPOSE Pulmonary transepithelial Na + transport is reduced by hypoxia, but in the airway regulatory mechanisms remain unclear. We investigated role of AMPK and ROS hypoxic regulation apical amiloride‐sensitive channels basolateral K ATPase activity. EXPERIMENTAL APPROACH H441 human epithelial cells were used to examine effects hypoxia on transport, AMP : ATP ratio Lentiviral constructs modify cellular abundance activity; pharmacological agents ROS. KEY RESULTS was activated...
Apical ATP, UTP and UDP evoked transient increases in short circuit current (I SC , a direct measure of transepithelial ion transport) confluent Caco‐2 cells grown on permeable supports. These responses were mediated by population at least three pharmacologically distinct receptors. Experiments using glass coverslips showed that ATP consistently increased intracellular free calcium ([Ca 2+ ] i ) whilst sensitivity to was variable. Cross desensitization experiments suggested the common...
Integration-deficient lentiviral vectors (IDLVs) are promising gene delivery tools that retain the high transduction efficiency of standard vectors, yet fail to integrate as proviruses and instead converted into episomal circles. These episomes metabolically stable support long-term expression transgenes in non-dividing cells, exhibiting a decreased risk insertional mutagenesis. We have embarked on an extensive study compare IDLVs pseudotyped with different envelopes (vesicular stomatitis,...
Schwannomas are tumors derived from Schwann-lineage cells, cells that protect and support myelinated nerves in the peripheral nervous system. They typically slow-growing, encapsulated benign. These develop along peripheral, spinal cranial causing pain, sensory-motor dysfunction death. Primary treatment for schwannoma is operative resection which can be associated with significant morbidity. Pharmacotherapy largely restricted to bevacizumab, has minimal or no efficacy many patients...
This study was conducted to evaluate the burden of HIV/AIDS on blood bank reserves at University Maiduguri Teaching Hospital, Nigeria, during 1995-2005. The number admissions due rose from 43 in 1995 447 2005, and proportion units used transfusing patients with steadily 3% 19% 2005. managing obstetric also high fluctuated between 36% 50% period study. Controlling spread HIV, effective treatment antiretroviral drugs setting up a functional national transfusion service could lessen rising...
Abstract Schwannomas are benign neoplasms that can cause gain‐ and loss‐of‐function neurological phenotypes, including severe, intractable pain. To investigate the molecular mechanisms underlying schwannoma‐associated pain we compared RNA sequencing profile of painful non‐painful schwannomas from NF2 patients. Distinct segregation tumors by gene expression patterns was observed. Differential analysis showed upregulation fibroblast growth factor 7 (FGF7) in schwannomas. Behavioral support for...
Background/Aim: This study evaluated the effect of enrofloxacin antibiotic treatment on ability an attenuated Salmonella typhimurium (S. typhimurium) strain VNP20009 to control schwannoma growth in a preclinical mouse tumor model. Materials and Methods: The antitumor efficacy intratumoral (i.t.) injection was assessed syngeneic mouse-NF2 model, with without subcutaneous (s.c.) beginning day-1 or day-8 post-VNP20009 injection. S. colonization excised samples following treatment. Results: I.t....
Objective: To study the pattern of deep vein thrombosis (DVT) and associated clinical risk factors in Maiduguri, Nigeria, with aim recognizing common need for prophylactic intervention. Materials Methods: Twenty-two (22) cases DVT seen at University Maiduguri Teaching Hospital (UMTH) from 1996 to 1999 were retrospectively analysed respect as documented diagnosis. Results: The frequencies individual among 22 patients studied included obesity 15 (68%) patients, abdominal operation 6 (27%)...
Dr Sagir G. Ahmed, Department of Haematology Aminu Kano Teaching Hospital PMB 3452, State, Nigeria. +2348034418015 drsagirahmed@yahoo.com Abstract lifestyle and nutritional culture the Africans are Global epidemiology shows that hypertension becoming increasingly westernized over time is severer, commoner occurs at relatively younger might be partly responsible for rising incidence nonage in black comparison to whites. communicable diseases, including hypertension, Since sickle cell trait...
A total of 12,540 homologous donors seen between 1993 and 1999 at the University Maiduguri Teaching Hospital (U.M.T.H) blood bank were analysed with respect to frequency viral infectivity markers (HBsAg HIV antibodies) as it relates donor categories. Fifteen percent 4.07% voluntary positive for HBsAg antibodies respectively. 4.11% family replacement respectively but did not differ significantly from frequencies in (P>0.05). Commercial had highest 21.3% 7.01% antibodies, higher than...
The Haemoglobin (Hb) genotypes of 1672 healthy blood donors at the university Maiduguri Teaching hospital (UMTH) during year 1999 were analysed. Hb AA, AS and SC detected in 78.94%, 21% 0.06% donor population respectively. There is need for strict application electrophoresis on all order to detect rare cases mild forms sickle cell disease who may present as donors. All banks should label their units with appropriate tags indicate genotype (Hb AA or AS) status donor; clinicians must...