A5041871611- Neuroscience and Neural Engineering
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Muscle activation and electromyography studies
- Conducting polymers and applications
- Trace Elements in Health
- Neurogenesis and neuroplasticity mechanisms
- Heavy Metal Exposure and Toxicity
- Tryptophan and brain disorders
- Transcranial Magnetic Stimulation Studies
- Virus-based gene therapy research
- Neurogenetic and Muscular Disorders Research
- Inflammation biomarkers and pathways
- Genetic and Kidney Cyst Diseases
- Hedgehog Signaling Pathway Studies
- Stress Responses and Cortisol
- Neuropeptides and Animal Physiology
- Signaling Pathways in Disease
- RNA regulation and disease
- Spinal Cord Injury Research
- Genetic Syndromes and Imprinting
- Immune Response and Inflammation
- Tissue Engineering and Regenerative Medicine
- Innovative Energy Harvesting Technologies
- Proteoglycans and glycosaminoglycans research
Universitat Autònoma de Barcelona
2002-2024
Centro de Investigación Biomédica en Red
2022-2024
Biomedical Research Networking Center on Neurodegenerative Diseases
2022-2024
Institut Pasteur de Montevideo
2012-2023
King's College London
2007-2012
Institut Pasteur
2011
Scripps Research Institute
2003
University of Copenhagen
2003
Longitudinal intrafascicular electrodes (LIFEs) are designed to be placed inside the peripheral nerve improve stimulation selectivity and increase recording signal-to-noise ratio. We evaluated functional morphological effects of either Pt wire LIFEs or polyimide-based thin-film implanted in rat sciatic for 3 mo. The newly more flexible, can micromachined allow placement active electrode sites than conventional LIFEs. Functional results at 1 mo indicated an initial decline conduction velocity...
Neuregulin-1 (NRG1) plays a crucial role in axoglial signaling during the development of peripheral nervous system, but its importance adulthood after nerve injury remains unclear. We used single-neuron labeling with inducible Cre-mediated knock-out animals, which enabled visualization subset adult myelinated sensory and motoneurons neurons Nrg1 was inducibly mutated by tamoxifen treatment. In uninjured mice, NRG1-deficient axons associated myelin sheath were normal, neuromuscular junction...
Lysophosphatidic acid (LPA) is an extracellular lipid mediator involved in many physiological functions that signals through six known G-protein-coupled receptors (LPA<sub>1</sub>–LPA<sub>6</sub>). A wide range of LPA effects have been identified the CNS, including neural progenitor cell physiology, astrocyte and microglia activation, neuronal death, axonal retraction, development neuropathic pain. However, little about involvement CNS pathologies. Herein, we demonstrate for first time...
We have demonstrated that micro-channel electrode arrays with 100 microm x cross-section channels support axon regeneration well, and micro-channels of similar calibre up to 5 mm long can vascularisation. They may be microfabricated using silicon, silicone, or polyimide thin metal films form 3-D bundles micro-channels. Arrays "mini-nerves," i.e., miniature nerve fascicles their own blood vessels, successfully grew through implants 0.5-5 long. Furthermore, guiding the regenerating fibres into...
Neural interfaces are implanted devices that couple the nervous system to electronic circuitry. They intended for long term use control assistive technologies such as muscle stimulators or prosthetics compensate loss of function due injury. Here we present a novel design interface peripheral nerves. Recording from axons is complicated by small size extracellular potentials and concentration current flow at nodes Ranvier. Confining microchannels ~100 µm diameter produces amplified independent...
Regenerative electrodes are designed to interface regenerated axons from a sectioned peripheral nerve. Applicability of regenerative depends on biocompatibility, success axonal regeneration, secondary nerve damage, and adequacy electronics. Polyimide sieve with 281 holes were chronically implanted in the severed sciatic 30 rats. Regeneration was successful all animals, increasing numbers myelinated fibers 2 6 mo. However, constrictive axonopathy affected few cases 12 postimplantation. A...
It has recently become evident that activating/inhibitory cell surface immune receptors play a critical role in regulating and inflammatory processes the central nervous system (CNS). The immunoreceptor CD300f expressed on monocytes, neutrophils, mast cells modulates inflammation, phagocytosis, outcome models of autoimmune demyelination, allergy, systemic lupus erythematosus. On other hand, finely regulated response is essential to induce regeneration after injury peripheral nerves since...
A role for microglia in neuropsychiatric diseases, including major depressive disorder (MDD), has been postulated. Regulation of microglial phenotype by immune receptors become a central topic many neurological conditions. We explored preclinical and clinical evidence the CD300f receptor fine regulation its contribution to MDD. found that prevalent nonsynonymous single-nucleotide polymorphism (C/T, rs2034310) human cytoplasmic tail inhibits protein kinase C phosphorylation threonine is...
Peripheral nerve injuries are rarely followed by complete return of function. Deficits particularly important for motor function, resulting in paralysis and muscle atrophy. In different groups, the sciatic was either crushed or transected repaired direct suture tube repair using silicone collagen tubes. After 60 days, regeneration assessed electrophysiological functional tests, morphology immunohistochemistry against choline acetyltransferase (ChAT) labeling axons. Suture resulted similar...
This paper reports on the design, in vitro and vivo investigation of a flexible, lightweight, polyimide based implantable sieve electrode with hybrid assembly multiplexers polymer encapsulation. The integration enables us to connect large number electrodes using few input connections. implant electronic circuitry was verified by impedance measurement. 27 platinum were coated black reduce impedance. magnitude sites (geometric surface area 2,200 microm(2)) |Z(f=1kHz)| = 5.7 kOmega. electrodes,...
Abstract Nerve regeneration after complete transection does not allow for adequate functional recovery mainly because of lack selectivity target reinnervation. We assessed if transplanting a nerve segment from either motor or sensory origin may improve specifically the accuracy and For this purpose, rat sciatic was transected repaired with silicone guide containing predegenerated ventral root (VR) dorsal (DR), compared to filled saline. reinnervation during 3 months by electrophysiologic...
Abstract It is well known that cell surface immune receptors play a critical role in regulating and inflammatory processes the central nervous system (CNS). We have analyzed function of cluster differentiation (CD)300f immunoreceptor model excitotoxic rat brain damage. First, to explore presence endogenous ligand(s) for this receptor we used human CD300f‐Ig soluble protein confocal microscopy, showing specific staining mainly CNS white matter on oligodendrocytes certain astrocytes. Next,...
Transgenic expression of interleukin-6 (IL-6) in the CNS under control glial fibrillary acidic protein (GFAP) gene promoter (GFAP-IL6 mice) causes significant damage and alters many genes, including a dramatic upregulation metallothionein-I (MT-I). The findings this report support idea that MT-I observed GFAP-IL6 mice is an important mechanism for coping with brain damage. Thus, were crossed TgMTI transgenic (GFAP-IL6xTgMTI) overexpressed showed decreased cytokines such as IL-6 diminished...
Longitudinal intrafascicular electrodes (LIFEs) designed to be placed inside a peripheral nerve seem adequate devices for constituting nerve-prosthesis interface. In this study we evaluated the functional and morphological effects of newly polyimide-based thin-film (tf-)LIFEs implanted in rat sciatic 3 months. The tf-LIFEs used are highly flexible, have 8 regularly spaced contacts, 4 at each side folded structure. Functional results 1 month showed mild decline conduction velocity amplitude...
Abstract In this study, we evaluated the long‐term maintenance of regenerated axons in an experimental nerve amputee model. The sciatic adult rats was transected and repaired with a silicone tube leaving short gap; distal segment again 10 mm distally stump either introduced capped chamber (amputee group) or connected to denervated targets (tibial branch into gastrocnemius muscle peroneal apposed skin) (reinnervation group). Morphological studies were performed at 2.5, 6, 9 months after...
Glial reactivity in the dorsal horn of spinal cord is a hallmark most chronic pain conditions. Neuroinflammation-associated reactive glia, particular astrocytes, have been shown to exhibit reduced mitochondrial respiratory function. Here, we studied function at lumbar tissue from complete Freund's adjuvant-induced inflammatory rat and constriction injury mouse models by high-resolution respirometry. A significant decrease bioenergetic parameters injury-related level coincided with highest...