A5075612442- Venomous Animal Envenomation and Studies
- Healthcare and Venom Research
- Hormonal and reproductive studies
- Pesticide Exposure and Toxicity
- Research on Leishmaniasis Studies
- Rabies epidemiology and control
- Bioactive Natural Diterpenoids Research
- Mosquito-borne diseases and control
- Sphingolipid Metabolism and Signaling
- Marine Invertebrate Physiology and Ecology
- Protein Kinase Regulation and GTPase Signaling
- Calpain Protease Function and Regulation
- Inflammatory mediators and NSAID effects
- Insect and Pesticide Research
- Lipid metabolism and biosynthesis
- Ion channel regulation and function
- Digital Marketing and Social Media
- Chemotherapy-induced organ toxicity mitigation
- Autoimmune and Inflammatory Disorders Research
- Blood Coagulation and Thrombosis Mechanisms
- Lower Extremity Biomechanics and Pathologies
- Allelopathy and phytotoxic interactions
- Hops Chemistry and Applications
- Pharmacological Effects and Assays
- Brazilian cultural history and politics
Universidade Federal de São Paulo
2017-2024
Universidade Cidade de São Paulo
2019
Universidade de São Paulo
2005-2019
Universidade Federal do Maranhão
2013-2017
Instituto Butantan
2006-2016
Universidade Estadual Paulista (Unesp)
2002
Centro Universitário de Araraquara
2002
Anthocyanins are flavonoids which demonstrated biological activities in vivo and vitro models. Here the anti-inflammatory properties of an anthocyanin-enriched fraction (AF) extracted from wild mulberry cyanidin-3-glucoside (C3G), most abundant anthocyanin diet, were studied two acute inflammation experimental models, peritonitis paw oedema assays, both induced by carrageenan (cg) mice. In each trial, AF C3G (4 mg/100 g/animal) orally administered distinct protocols: 30 min before 1 h after...
Leishmaniasis is a neglected tropical disease caused by >20 species of the protozoan parasite Leishmania Meglumine antimoniate (Glucantime) first-choice drug recommended World Health Organization for treatment all types leishmaniasis. However, mechanisms action and toxicity pentavalent antimonials, including genotoxic effects, remain unclear. Therefore, mechanism which meglumine causes DNA damage was investigated BALB/c mice infected (Leishmania) infantum treated with (20 mg/kg 20 days)....
The snake venom MT-III is a group IIA secreted phospholipase A2 (sPLA2) enzyme with functional and structural similarities mammalian pro-inflammatory sPLA2s of the same group. Previously, we demonstrated that directly activates innate inflammatory response macrophages, including release mediators formation lipid droplets (LDs). However, mechanisms coordinating these processes remain unclear. In present study, by using TLR2−/− or MyD88−/− C57BL/6 (WT) male mice, report TLR2 MyD88 signaling...
Phospholipases A2 (PLA2) are key enzymes for production of lipid mediators. We previously demonstrated that a snake venom sPLA2 named MT-III leads to prostaglandin (PG)E2 biosynthesis in macrophages by inducing the expression cyclooxygenase-2 (COX-2). Herein, we explored molecular mechanisms and signaling pathways leading these MT-III-induced effects. Results induced activation transcription factor NF-κB isolated macrophages. By using selective inhibitors, involvement this COX-2 PGE2 was...
MT-II, a Lys49PLA2 homologue devoid of catalytic activity from B. asper venom, stimulates inflammatory events in macrophages. We investigated the ability MT-II to induce formation lipid droplets (LDs), key elements responses, isolated macrophages and participation protein kinases intracellular PLA2s this effect. Influence on PLIN2 recruitment expression was assessed, effects some synthetic peptides LD were further evaluated. At noncytotoxic concentrations, directly activated form LDs. This...
Abstract Crotoxin B (CB) is a catalytically active group IIA sPLA 2 from Crotalus durissus terrificus snake venom. In contrast to most GIIA s, CB exhibits anti-inflammatory effects, including the ability inhibit leukocyte functions. Lipid droplets (LDs) are lipid-rich organelles associated with inflammation and recognized as site for synthesis of inflammatory lipid mediators. Here, induce formation LDs mechanisms involved in this effect were investigated isolated macrophages. The profile...
Long-term nonsteroidal anti-inflammatory drugs (NSAIDs) therapy has been associated with several adverse effects such as gastric ulceration and cardiovascular events. Among the molecular modifications strategies, prodrug approach is a useful tool to discover new safe NSAIDs. The 1-(2,6-dichlorophenyl)indolin-2-one diclofenac which demonstrated relevant properties without gastro effect. In addition, decreases PGE2 levels, COX-2 expression cellular influx into peritoneal cavity induced by...
Abstract Despite being considered ergolytic drugs, diuretics have been included on the lists of prohibited substances International Olympic Committee since 1986 due to their capacity masking doping agents in urine. As are also prone be misused regarding weight categories, screening procedures check presence urine, whenever control is involved, mandatory. A simple method using liquid‐liquid extraction was validated for compounds: acetazolamide, amiloride, bumetanide, chlorthalidone,...
Protective effects of antileishmanial extract (2000 mg/kg) 24 h before or after receiving cyclophosphamide (50 mg/kg), respectively.The had no genotoxic in the vitro vivo assays.However, reduced apoptotic cell death and induced necrotic at concentrations that presented leishmanicidal activity vitro.The also an antigenotoxic effect, reducing levels genomic damage were caused by Swiss mice more than 80%.
Evaluation of genotoxic effects antileishmanial extract damage caused by cyclophosphamide.Hydroalcoholic extracts J. triqueter did not provoke DNA at concentrations and doses normally used for treatment; however, they reduced apoptotic cell death induced necrotic death.