A5101410499- Tissue Engineering and Regenerative Medicine
- Cardiac Valve Diseases and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Computational Drug Discovery Methods
- Fungal Biology and Applications
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Cholinesterase and Neurodegenerative Diseases
- Pesticide Exposure and Toxicity
- Monoclonal and Polyclonal Antibodies Research
- Thyroid Disorders and Treatments
- Dermatology and Skin Diseases
- Polysaccharides and Plant Cell Walls
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Receptor Mechanisms and Signaling
- Nuclear Structure and Function
- Aortic Disease and Treatment Approaches
- Cardiac Structural Anomalies and Repair
- Plant Pathogens and Fungal Diseases
- Nail Diseases and Treatments
University of Leeds
2008-2022
RF Laboratories (United States)
2019
Université de Technologie de Compiègne
1987
Centre National de la Recherche Scientifique
1987
Malassezia spp. are commensal, cutaneous fungi that implicated in seborrhoeic dermatitis. We hypothesize the lipid-rich capsule of masks organism from host detection, and depletion this layer elicits an inflammatory response. To test this, preparations capsulated or acapsular [10% (v/v) Triton X-100 treated], viable nonviable, exponential stationary phase furfur, globosa, obtusa, restricta, slooffiae sympodialis, were incubated with normal human keratinocytes. Proinflammatory (IL-6, IL-8,...
The kinetics of the hydrolysis butyrylthiocholine by horse serum butyrylcholinesterase (acylcholine acylhydrolase; BuChE; EC 3.1.1.8) exhibit an activation phenomenon at high substrate concentrations. At least two mechanistic models can account for enzyme kinetics: one assumes binding additional molecule on acyl–enzyme intermediate, and other hypothesizes existence a peripheral regulatory site substrate. (1-Dimethylaminonaphthalene-5-sulfonamidoethyl)-trimethylammonium perchlorate, potent...
The crystal structures of the thyroid-stimulating hormone receptor (TSHR) leucine-rich repeat domain (amino acids 22-260; TSHR260) in complex with a stimulating human monoclonal autoantibody (M22TM) and blocking (K1-70™) have been solved. However, attempts to purify crystallise free TSHR260, that is not bound an autoantibody, unsuccessful due poor stability TSHR260. We now describe TSHR260 mutant has stabilised by introduction six mutations (H63C, R112P, D143P, D151E, V169R I253R) form...
Abstract The aim of this study was to investigate the suitability decellularised porcine pericardium for heterotopic repair mitral valve (MV) leaflets, and its potential regenerate through endogenous cell repopulation in vivo, or vitro seeding bioreactor conditioning. Anterior posterior MV leaflets pericardia were excised from hearts within. according in-house protocol. leaflet, fresh pericardial samples subjected histology (H E, Masson trichrome, Sirius Red, Miller’s elastin, Alcian...
The primary objective was to evaluate performance of low concentration SDS decellularised porcine pulmonary roots in the right ventricular outflow tract juvenile sheep. Secondary objectives were explore cellular population over time. Animals monitored by echocardiography and explanted at 1, 3, 6 ( n = 4) 12 months 8) for gross analysis. Explanted subject histological, immunohistochemical quantitative calcium analysis 4 3 months) determination material properties 4; months). Cryopreserved...
Human sperm parameters serve as a first step in diagnosing male infertility, but not determining the potential for successful pregnancy during assisted reproductive technologies (ARTs) procedures. Here, we investigated relationship between head morphology at high magnification, based on strict morphologic criteria, and nuclear architecture analyzed by fluorescence situ hybridization (FISH). We included five men. Two of them had an elevated high-magnification score 6 points (Score 6)...
Ultrastructural examination of free and calcium alginate immobilized Gibberella fujikuroi mycelia showed that in addition to the changes occurring during transition phase from primary secondary metabolism, there are several alterations ultrastructure hyphae as a response microenvironmental owing immobilization constraints. Internal included (i) presence large glyoxisomelike bodies active vesicle-generating systems, which appeared cloudy structures electron micrographs; (ii) formation...
Mitral valve regurgitation represents the second major valvular disorder in western world, whereas current strategies for mitral reconstruction are imperfect. The aim of this study was to develop a tissue engineered substitute leaflet using acellular porcine pericardium seeded with mesenchymal stem cells (pMSCs).
MethodsEAC were decellularized by a detergent-based protocol for 40h (40h-dEAC) or 72h (72h-dEAC) and coated with without 100ng/ml CCN1.Subsequently, THP-1 cells seeded onto the scaffolds 24 hours.For negative positive controls on tissue culture plastic stimulated 100nM PMA.Expression of pro-inflammatory cytokines TNF-alpha, MIP-1alpha, MCP-1 IL-1beta anti-inflammatory IL1-ra IL-10 determined using quantitative reverse transcriptase PCR. ResultsCytokine expression in uncoated showed 40h-dEAC...