A5033441609- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Viral Infections and Immunology Research
- CRISPR and Genetic Engineering
- Polyamine Metabolism and Applications
- Cancer-related gene regulation
- RNA regulation and disease
- Bacteriophages and microbial interactions
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Peptidase Inhibition and Analysis
- RNA Interference and Gene Delivery
Engelhardt Institute of Molecular Biology
2020-2025
Abstract The poly(A) tail plays an important role in maintaining mRNA stability and influences translation efficiency via binding with PABP. However, the impact of length on remains incompletely understood. This study explores effects human translation. We determined rates cell lysates using mRNAs different tails. Cap-dependent was stimulated by tail, however, it largely independent length, exception observed case 75 nt tail. Conversely, cap-independent displayed a positive correlation...
Eukaryotic translation initiation factor 4F (eIF4F), comprising subunits eIF4G, eIF4E, and eIF4A, plays a pivotal role in the 48S preinitiation complex assembly ribosomal scanning. Additionally, eIF4B enhances helicase activity of eIF4A. eIF4F also interacts with poly (A)-binding protein (PABP) bound to (A) tail messenger RNA (mRNA), thereby forming closed-loop structure. PABP, turn, eukaryotic release 3 (eRF3), stimulating termination. Here, we employed reconstituted mammalian system...
The nucleotide context surrounding stop codons significantly affects the efficiency of translation termination. In eukaryotes, various 3′ contexts that are unfavorable for termination have been described; however, exact molecular mechanism mediates their effects remains unknown. this study, we used a reconstituted mammalian system to examine in different contexts, including several previously described weak codon contexts. We developed an approach estimate level readthrough absence...
Nsp1 of SARS-CoV-2 regulates the translation host and viral mRNAs in cells. inhibits initiation by occluding entry channel 40S ribosome subunit. The structural study Nsp1-ribosomal complexes reported post-termination 80S complex containing Nsp1, eRF1 ABCE1. Considering presence ribosomal complex, we hypothesized that may be involved termination. Using a cell-free system reconstituted vitro system, show stimulates peptide release formation termination complexes. Detailed analysis activity...
Programmed cell death 4 protein (PDCD4) regulates many vital processes, although is classified as a tumor suppressor because it inhibits neoplastic transformation and growth. For example, PCDC4 has been implicated in the regulation of transcription mRNA translation. PDCD4 known to inhibit translation initiation by binding eukaryotic factor 4A elongation oncogenic c- A-myb mRNAs. Additionally, shown interact with poly(A)-binding (PABP), which affects termination, significance this interaction...
ABSTRACT Nonsense variants underlie many genetic diseases. The phenotypic impact of nonsense is determined by nonsense-mediated mRNA decay (NMD), which degrades transcripts with premature termination codons (PTCs). Despite its clinical importance, the factors controlling transcript-specific and context-dependent variation in NMD activity remain poorly understood. Through analysis human datasets, we discovered that amino acid preceding PTC strongly influences activity. Notably, glycine...
Abstract Eukaryotic translation release factor eRF1 is an important cellular protein that plays a key role in termination, nonsense-mediated mRNA decay (NMD), and readthrough of stop codons. The amount the cell influences all these processes. mechanism regulation through autoregulatory NMD-dependent expression circuit has been described for plants fungi, but mechanisms human have not yet studied. Using reporter constructs, we studied effect elements on its cell-free systems HEK293 culture....
Abstract eIF3j is one of the eukaryotic translation factors originally reported as labile subunit initiation factor eIF3. The yeast homolog this protein, Hcr1, has been implicated in stringent AUG recognition well controlling termination and stop codon readthrough. Using a reconstituted mammalian vitro system, we showed that human protein also important for termination. We stimulates peptidyl-tRNA hydrolysis induced by complex release factors, eRF1-eRF3. Moreover, combination with eIF3,...
Abstract The genetic code is a set of instructions that determine how the information in our material translated into amino acids. In general, it universal for all organisms, from viruses and bacteria to humans. However, last few decades, exceptions this rule have been identified both pro‐ eukaryotes. review, we discuss 16 described alternative eukaryotic nuclear codes observe theories their appearance evolution. We consider possible molecular mechanisms allow codon reassignment. Most...
Eukaryotic release factor eRF1, encoded by the ETF1 gene, recognizes stop codons and induces peptide during translation termination. produces several different transcripts as a result of alternative splicing, from which two eRF1 isoforms can be formed. Isoform 1 codes well-studied canonical isoform 2 is 33 amino acid residues shorter than completely unstudied. Using reconstituted mammalian in vitro system, we showed that human also involved translation. We eRF1iso2 interact with ribosomal...
ABSTRACT Eukaryotic translation initiation factor eIF4F, comprising subunits eIF4G, eIF4E, and eIF4A, plays a pivotal role in the 48S preinitiation complex assembly ribosomal scanning. Additionally, eIF4B enhances helicase activity of eIF4A. eIF4F also interacts with PABP bound to poly(A) tail mRNA, thereby forming closed-loop structure. PABP, turn, eRF3, stimulating termination. Here, we employed reconstituted mammalian system directly demonstrate that potently Specifically, eIF4A promote...
Translation termination factor eRF1 is an important cellular protein that plays a key role in translation termination, nonsense-mediated mRNA decay (NMD), and stop-codons readthrough. An amount of the cell influences all these processes. The mechanism regulation through autoregulatory NMD-dependent expression circuit has been described for plants fungi, but mechanisms human have not yet studied. Using reporter constructs, we studied effect elements on its cell-free systems HEK293 cells. Our...
ABSTRACT The Nsp1 protein of SARS-CoV-2 regulates the translation host and viral mRNAs in cells. inhibits initiation by occluding entry channel 40S ribosome subunit. structural study Nsp1-ribosomal complexes reported post-termination 80S complex containing eRF1 ABCE1 proteins. Considering presence ribosomal simultaneously with eRF1, we hypothesized that may be involved termination. Using a cell-free system reconstituted vitro system, show stimulates termination stop codon recognition stage...