A5017512554- Epigenetics and DNA Methylation
- Machine Learning in Bioinformatics
- Drug-Induced Adverse Reactions
- Drug-Induced Hepatotoxicity and Protection
- Metabolomics and Mass Spectrometry Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Tuberculosis Research and Epidemiology
- Computational Drug Discovery Methods
- HIV/AIDS drug development and treatment
- Autoimmune Bullous Skin Diseases
- vaccines and immunoinformatics approaches
- Glycosylation and Glycoproteins Research
- Pharmacogenetics and Drug Metabolism
- Dermatoglyphics and Human Traits
- Hemoglobinopathies and Related Disorders
- Gut microbiota and health
- Plant Gene Expression Analysis
- Carcinogens and Genotoxicity Assessment
- Gene expression and cancer classification
- Child Nutrition and Water Access
- Chemical Synthesis and Analysis
- Mast cells and histamine
- DNA Repair Mechanisms
- Animal Genetics and Reproduction
- Polysaccharides and Plant Cell Walls
Yarsi University
2015-2024
Bandung Institute of Technology
2015
Carbamazepine (CBZ) is a common cause of life-threatening cutaneous adverse drug reactions such as Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN). Previous studies have reported strong association between the HLA genotype CBZ-induced SJS/TEN. We investigated SJS/TEN in Javanese Sundanese patients Indonesia. Nine unrelated with 236 healthy controls were genotyped for HLA-B their allele frequencies compared. The HLA-B*15:02 was found 66.7% SJS/TEN, but only 29.4% tolerant...
Pharmacogenomics can enhance the outcome of treatment by adopting pharmacogenomic testing to maximize drug efficacy and lower risk serious adverse events. Next-generation sequencing (NGS) is a cost-effective technology for genotyping several loci at once, thereby increasing publicly available data. A panel 100 pharmacogenes among Southeast Asian (SEA) populations was resequenced using NGS platform under collaboration Research Network (SEAPharm). Here, we present frequencies variants...
BackgroundThe HLA-B alleles have been used as a marker to predict drug-induced adverse reactions and major contributor hypersensitivity reactions. We examined the feasibility of pharmacogenomic markers in an Indonesian Malay Ethnic.MethodsFifty-eight individuals ethnicity were enrolled this study. determined using reverse sequence-specific oligonucleotide probe coupled with xMAP technology.ResultsHLA-B*15:02 (15.52%), HLA-B*35:05 (9.48%), HLA-B*07:05 (7.76%) frequent ethnic populations....
Aim: We investigated the contribution of NAT2 variants and acetylator status to anti-tuberculosis drug-induced liver injury (AT-DILI) severity. Materials & methods: 100 patients with clinically severe AT-DILI 210 non-AT-DILI controls were subjected genotyping by direct DNA sequencing. Results: slow was significantly associated risk (p = 2.7 × 10-7; odds ratio [95% CI] 3.64 [2.21-6.00]). Subgroup analysis ultra-slow revealed a stronger association 4.3 10-6; 3.37 [2.00-5.68]). Subset severity...
Background: Arylamine N-acetyltransferase 2 (NAT2) polymorphism was previously reported to have association with the risk of drug toxicities and development various diseases. Previous research on Indonesian population, especially Javanese Sundanese, showed that there were 33% NAT2 slow acetylator phenotype. The aim this study map variation in Malay ethnic gain a deeper insight into haplotypic composition ethnic.Methods: 50 healthy samples from obtained. They interviewed about their...
Background: Owing to the high resistance rate of tuberculosis (TB) isoniazid, which is metabolized by N-acetyltransferase 2 (NAT2), we investigated associations between NAT2 variants and multidrug-resistant (MDR)-TB. Materials & methods: The acetylator status based on haplotypes 128 patients with MDR-TB in Indonesia were compared our published data from anti-TB drug-induced liver injury (AT-DILI), TB general population. Results: NAT2*4 was more frequent group than AT-DILI group, controls...
Tuberculosis (TB) is still a major public health problem in Indonesia. Anti-tuberculosis drug-induced hepatotoxicity (DIH) common side effect leading to changes treatment regimens, and the less effective second-line treatments. Several risk factors such as age, sex, body mass index (BMI) acetylization status for were suggested previous studies but fact, those are often not related DIH incidence after receiving standard TB regimen. The aim of this study was asses role pulmonary patients...
N-acetyltransferase 2 (NAT2) is a key enzyme involved in the phase II metabolism of aromatic amines and heterocyclic present wide range xenobiotics. The aim this study was to investigate NAT2 polymorphism Buginese ethnic group Indonesia determine frequency alleles population.We found six haplotypes consisting single-nucleotide polymorphisms 12 genotype variations. NAT2*6A haplotype (42%) showed highest frequency, followed by NAT2*4 (33%), NAT2*7B (15%), NAT2*5B (5%), NAT2*12A (3%), NAT2*13...
Asma adalah penyakit pernafasan yang ditandai oleh obstruksi saluran napas disebabkan peradangan bronkus akut dan kronis. Gen disintegrin and metalloprotease 33 (ADAM33) merupakan gen terkait kerentanan terhadap asma diketahui memiliki lebih dari 300 polimorfisme. Studi meta-analisis melaporkan bahwa rs2280091, rs2787094, rs511898, rs2280089 rs2280090 asosiasi kuat dengan pada populasi Asia. Penelitian pendahuluan ini mengumpulkan 10 partisipan sehat untuk mengidentifikasi alel genotipe...
Stunting is the result of chronic malnutrition due to lack micronutrient-based methyl donors required for epigenetic programming during first 1000 days life. Methylation studies using bisulfite conversion from blood DNA are invasive and may not be practical large-scale epidemiological investigation or nutrition intervention programs. Buccal epithelial methylation reflect early germline methylation. Therefore, buccal cells can serve as convenient sample sources collect biomarkers associated...
BackgroundDetermination of the acetylator type NAT2 generally can be predicted based on genotype data from database.However, in some reported studies, it does not show 100 per cent concordance with phenotype urinary assay.The assay only differentiates rapid and slow but consider intermediate one. AimsWe conducted this study to define both genotyping determine rate between methods groups.
Banana is classified as a climateric fruit, whose ripening regulated by ethylene. Ethylene synthesized from ACC (1-aminocyclopropane-1-carboxylic acid) oxidase enzyme which encoded ACO gene. Controling an important gene expression in ethylene biosynthesis pathway has became target to delay the process. Therefore previous study we have designed MaACO-RNAi construct control MaACO expression. In this research, effectiveness of different transient transformation methods deliver construct. Direct...
Human leucocyte antigen (HLA) adalah protein penyaji yang lokus genetiknya berada di kromosom 6p21 dengan ukuran sebesar 3,8 Mb dan berasosiasi lebih dari 100 penyakit berbeda kebanyakan merupakan autoimun. Proses HLA-typing menggunakan sekuensing Sanger masih memberikan ambiguitas terhadap determinasi alel, low-throughput, membutuhkan biaya besar untuk sampel dalam jumlah besar. Next generation sequencing (NGS) menjadi metode dapat mengatasi kelemahan Sanger. MiSeq Illumina salah satu NGS...
Beta-thalassemia merupakan gangguan hematologis autosomal yang secara genetis mengakibatkan berkurangnya sintesis beta-globin di hemoglobin. Beta-talasemia sebagian besar disebabkan oleh mutasi titik, insersi atau delesi dalam gen terletak pada lengan pendek kromosom 11. Organisasi Kesehatan Dunia (WHO) memperkirakan terdapat sekitar 1,5% dari populasi global (80-90 juta orang) adalah pembawa ?-thalassemia. Tidak ada studi komprehensif untuk mendeteksi beta-thalassemia Indonesia, terutama...
Past studies have delved into the genetic factors underlying anti-tuberculosis drug-induced liver injury (AT-DILI), primarily concentrating on polymorphisms in genes responsible for drug-metabolizing enzymes. However, immune system's potential impact drug adverse effects, specifically through such as HLA, has received limited attention. Previous research notably revealed an association between HLA-DQB1*05 and AT-DILI, prevalence of HLA-DQB*05:02 allele AT-DILI patients. In light this, our...
The N-acetyltransferase 2 (NAT2) polymorphism in coding region has been studies intensively. However, there are limited for promoter of NAT2 gene. Several reported study showed that the gene is genotyped by PCR-sequencing approach. In this paper, we describe TaqMan based assays genotyping with following SNP: rs4646243 [T>C], rs4646244 [T>A], rs4646267 [A>G], rs4345600 and rs4646246 [A>G]. Our result a good separation cluster, trailing cluster some mix cluster. assay shown...
Tuberculosis (TB) remains Indonesia's leading infectious disease. Hepatotoxicity is the most common side effect of TB first-line medication therapy in patients. GSTM1 and GSTT1 are glutathione S-transferase (GST) genes involved detoxification various toxic compounds such as drugs. The development fast simple methods for null genotyping GSTM1/GSTT1 could facilitate large pharmacogenetic studies clinical application personalized drug dose adjustment according to patient's genetic profile. aim...
The rs1800629 polymorphism plays a crucial role in the pathogenesis of infectious and autoimmune diseases. Meanwhile, tuberculosis (TB) remains health primary disease Indonesia. purpose this study is to evaluate HRM method detecting genotype, TNF-α gene’s promoter region, within TB patients. benefit accelerate detection with simple, rapid, cost-effective for genotyping mutation screening that does not include use fluorescent probe. In experimental study, genotyped total 25 patients using...
Asthma is a respiratory disease caused by narrowing of the bronchial tubes, causingshortness breath. multi-factorial disorder resulting from combination ofgenetic and environmental factors. impact on productivity because most patientsexperiencing symptoms that affect their daily lives. Dermatoglyphs, patterns skin ridges,are derived hypodermal neural system formed embryologically between the10th 17th weeks. Student can be measured Grade Point Average(GPA). In this study we determine...